NIGHTSEA Stereo Microscope Fluorescence Adapter
NIGHTSEA Stereo Microscope Fluorescence Adapter
Mouse with actin labeled with GFP. Photo © NIGHTSEA™/Charles Mazel
Mouse with actin labeled with GFP. Photo © NIGHTSEA™/Charles Mazel
Mouse with actin labeled with DsRed. Photo © NIGHTSEA™/Charles Mazel
Mouse with actin labeled with DsRed. Photo © NIGHTSEA™/Charles Mazel
GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel
GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel
NIGHTSEA adapter
NIGHTSEA adapter
NIGHTSEA microscope adapter
NIGHTSEA microscope adapter
NIGHTSEA™ FLUORESCENCE VIEWING SYSTEMS

NIGHTSEA™ Stereo Microscope Fluorescence Adapter

Adapt your existing lab stereo microscopes for fluorescence

The Nightsea™ stereo microscope fluorescence adapter adapts just about any stereo microscope (dissecting microscope) for fluorescence with no modification to the microscope itself. The modular design lets you easily switch between several different excitation/emission combinations to work with a variety of fluorescent proteins and other fluorophores. There are now six different excitation/emission combinations available, plus white light.


Applications

This simple system is excellent for:

  • Quick screening of your fluorescent genotypes -Drosophila, zebrafish, C. elegans,...
  • Genotype sorting
  • Fluorescence-aided dissection, injection, or micromanipulation
  • Freeing up your research-grade fluorescence microscopes for more demanding work
  • New faculty start-up budgets
  • Bringing fluorescence into the teaching laboratory

Mouse with actin labeled with GFP. Photo © NIGHTSEA™/Charles Mazel
Mouse with actin labeled with DsRed. Photo © NIGHTSEA™/Charles Mazel
GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel


Fluorescence isn't just for research microscopes anymore

  • Now sort on your laboratory-level stereos
  • Use to facilitate micromanipulation and dissection
  • Expand from your research lab to your classroom

Modular

  • Installs in seconds - just clicks into place
  • Interchangeable excitation/emission combinations
  • Move from microscope to microscope
  • No modification to your micoscope needed

Economical - more glow for the dough

  • Stretch your lab budget
  • Inexpensive enough for classroom use

Grows as your lab grows

  • Buy just what you need now (up to 5 different wavelength sets)
  • Add more as your needs expand

The stereo microscope fluorescence adapter system consists of

  • Flexible gooseneck lamp base with power supply
  • Light head
  • Ring adapter for microscope
  • Barrier filter
  • Filter shield

The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light filter combinations.

The microscope mounting adapter fits up to 67mm to work with the majority of stereo microscopes.

Once you are set up for one excitation/ emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.


Specifications

Filter SetExcitationEmissionFluorophores
RB - Royal Blue440-460nm500nm LPGFP, eGFP, fluorescein...
RB-GO440-460nm500-560nm BPGFP, eGFP, fluorescein...
CY - Cyan490-515nm550nm LPYFP, Venus, Lucifer Yellow...
GR - Green510-540nm600nm LPDsRed, dTomato...
VI - Violet400-415nm460nm LPCFP, ...
UV - Ultra Violet360-380nm415nm LPDAPI, ...

Microscope Mounting Adapter - fits up to 67mm standard.


Green-only barrier filter

The green-only barrier filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are long-pass filters this new filter is a bandpass, transmitting from approximately 500 to 560nm. The long-pass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.

OSFA-CY
Nightsea fluorescence viewing system with cyan
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OSFA-GR
Nightsea fluorescence viewing system with green
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OSFA-RB
Nightsea fluorescence viewing system with royal blue
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OSFA-RB-GO
Nightsea fluorescence viewing system with royal blue light head, green-only
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OSFA-UV
Nightsea fluorescence viewing system with ultra violet
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OSFA-VI
Nightsea fluorescence viewing system with violet
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NIGHTSEA ACCESSORIES
Eclipse MicroTent
Eclipse MicroTent
ECLIPSE MICROTENT

The Eclipse MicroTent™ is a unique product for fluorescence microscopy that provides local darkness around conventional laboratory stereo microscopes. Fluorescence microscopes are kept in dark rooms for good reason - fluorescence can be weak and in many cases it can be difficult to see well if there is any ambient light. Microscopes may be on lab benches in shared spaces, near windows, or in other difficult-to-darken locations such as in the field. Turning off overhead lights can help but inconveniences others.

The Eclipse Microtent creates local darkness around a microscope while still providing easy access to the sample stage and the focus and zoom controls. It is designed for stereo microscopes but could potentially be used with many varieties of compound microscopes.


Features

  • Opening for the microscope oculars with elastic sleeve to minimize light entry
  • Large front flap provides easy access to the sample stage and can fasten open
  • Arm slots on sides to provide access to focus and zoom controls
  • User-customizable feedthrough patches to provide additional penetrations for camera port, power cords, CO? lines, or other features as you need
  • Tru-Block™ Eye Shields included with every Eclipse MicroTent
  • Folds flat for storage
  • Dimensions: 460 x 300 x 500mm
OSFA-TENT
Nightsea Eclipse MicroTent™
AUD615.00
ADD TO CART 
Eye Shields
Eye Shields
EYE SHIELDS

Light entering your eyes from the side can interfere with what you want to see in microscopy in general, and fluorescence microscopy in particular. Eye cups are available, but the standard ones don't extend far from the microscope and don't do a good job as ambient light increases. Our soft, molded rubber high-sided microscope eye shields are the answer. The tall wings extend up far enough to truly shield your eyes from any level of ambient light and eliminate distractions so that you can see your subject better. Two pairs (one Standard, one Compact) will be included with every Eclipse MicroTent™, and you can also purchase them separately.


The Tru-Block Eye Shields are available in two sizes:

  • Standard - fits 36 - 45mm
  • Compact - fits 28 - 37mm
OSFA-EYE-C
Nightsea tru-block eye shields, compact
AUD45.00
set
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OSFA-EYE-S
Nightsea tru-block eye shields, standard
AUD45.00
set
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Add-on light and filter set
Add-on light and filter set
Add-on light and filter set
ADD-ON LIGHT AND FILTER SETS

Each add‐on excitation/emission set consists of:

  • Light Head*
  • Barrier Filter
  • Viewing Shield
  • Padded Storage Box

*Please note: Light Head connectors come in two types. They are not interchangeable. The new style connector is called a BNC, and the original connector is a V1. There are two differences ‐ the connector on the end of the gooseneck and the mating connector on the back of the light head. It is easy to tell them apart:

  • The BNC connector on the gooseneck is silver, while the V1 connector is black.
  • The BNC light heads have the letters BNC printed on the side.
BNC vs V1 connectors
BNC vs V1 connectors
OSFA-BLFS-GO
Nightsea BNC add-on light and filter set, green-only
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OSFA-LFS-CY
Nightsea add-on light and filter set, cyan
AUD924.00
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OSFA-LFS-GO
Nightsea add-on light and filter set, green-only
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OSFA-LFS-GR
Nightsea add-on light and filter set, green
AUD924.00
ADD TO CART 
OSFA-LFS-RB
Nightsea add-on light and filter set, royal blue
AUD924.00
ADD TO CART 
OSFA-LFS-UV
Nightsea add-on light and filter set, ultra violet
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OSFA-LFS-VI
Nightsea add-on light and filter set, violet
AUD834.00
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Modular White Light Head
Modular White Light Head
MODULAR WHITE LIGHT HEAD

A white light head is also available as an illumination option for the Stereo Microscope Fluorescence Adapter. Now if you are using the NIGHTSEA system for fluorescence you do not need a separate light source for your white-light work - just exchange the fluorescence excitation light head module for the white-light module in a matter of seconds. Extremely convenient for general illumination and as a focusing aid for fluorescence imaging.

OSFA-LH-CY
Nightsea light head, cyan
AUD472.00
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OSFA-LH-GR
Nightsea light head, green
AUD472.00
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OSFA-LH-RB
Nightsea light head, royal blue
AUD472.00
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OSFA-LH-UV
Nightsea light head, ultra violet
AUD725.00
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OSFA-LH-VI
Nightsea light head, violet
AUD472.00
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NIGHTSEA adapter
NIGHTSEA adapter
NIGHTSEA™ A LA CARTE

Need an extra, not a set? Order from here:

OSFA light head, barrier filter, and shield
OSFA light head, barrier filter, and shield
OSFA excitation/emission sets, plus white light head
OSFA excitation/emission sets, plus white light head
OSFA-AD
Nightsea adapter
AUD343.00
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OSFA-BASE
Nightsea base
AUD736.00
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OSFA-BF-CY
Nightsea barrier filter, cyan
AUD399.00
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OSFA-BF-GO
Nightsea barrier filter, green only
AUD399.00
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OSFA-BF-GR
Nightsea barrier filter, green
AUD399.00
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OSFA-BF-RB
Nightsea barrier filter, royal blue
AUD399.00
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OSFA-SH-CY
Nightsea shield, cyan
AUD216.00
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OSFA-SH-GO
Nightsea shield, green only
AUD216.00
BACKORDER 
OSFA-SH-GR
Nightsea shield, green
AUD216.00
BACKORDER 
OSFA-SH-RB
Nightsea shield, royal blue
AUD216.00
BACKORDER 
NIGHTSEA™ FLUORESCENCE EXCITATION FLASHLIGHTS

Rapid screening of your fluorescent transgenic experiments

NIGHTSEA DFP-1 Dual Fluorescent Protein Flashlight
NIGHTSEA DFP-1 Dual Fluorescent Protein Flashlight
Dual Fluorescent Protein Flashlight
You can easily screen for two different colored fluorophores with one flashlight. Green (GFP) and Red (DsRed) Fluorescent Mice Green (GFP) and Red (DsRed) Fluorescent Mice
You can easily screen for two different colored fluorophores with one flashlight. Green (GFP) and Red (DsRed) Fluorescent Mice Green (GFP) and Red (DsRed) Fluorescent Mice
NIGHTSEA DFP™ DUAL FLUORESCENT PROTEIN FLASHLIGHT

The Model DFP Dual Fluorescent Protein Flashlights include two different, switch-selectable high intensity LEDs for excitation of fluorescent proteins. For years the most popular fluorescent colors have been green (GFP, eGFP, fluorescein, etc.) and red (DsRed, TdTomato, etc.), and we offered only one version of the DFP, with a combination of blue and green LEDs for exciting these fluorophores. This is still our 'standard' DFP product, but we are now offering build-to-order combinations using any two of the excitation colors that are available with our popular Model SFA Stereo Microscope Fluorescence Adapter.

With every DFP light you will receive two pairs of barrier filter glasses and a convenient storage case. The glasses are well matched to the excitation so that they block the reflected excitation light while transmitting the fluorescence with high efficiency, providing excellent viewing contrast.


Specifications

Filter SetColourExcitationEmissionFluorophores
RB - Royal Blue440-460nm500nm LPGFP, fluorescein, lucifer yellow, ...
CY - Cyan490-515nm550nm LPYFP, Venus, ...
GR - Green510-540nm600nm LPDsRed, TdTomato, RFP, ...
VI - Violet400-415nm460nm LPBFP, CFP, ...
UV - Ultra Violet360-380nm415nm LPDAPI, ...
Dimensions5 x 3 x 2
Battery Type4 C-cell
Bulb TypeTwo high intensity 3W LED
Burn Time4 hours at full power
Lamp Life10,000 hours

Dual Fluorescent Protein Flashlight side
Dual Fluorescent Protein Flashlight set
ODFP-1
DFP Flashlight with RB and GR LEDs, matching filter glasses, carrying case
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ODFP-CG
DFP Flashlight with CY and GR LEDs, matching filter glasses, carrying case
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ODFP-RC
DFP Flashlight with RB and CY LEDs, matching filter glasses, carrying case
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ODFP-UC
DFP Flashlight with UV and CY LEDs, matching filter glasses, carrying case
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ODFP-UG
DFP Flashlight with UV and GR LEDs, matching filter glasses, carrying case
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ODFP-UR
DFP Flashlight with UV and RB LEDs, matching filter glasses, carrying case
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ODFP-UV
DFP Flashlight with UV and VI LEDs, matching filter glasses, carrying case
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ODFP-VC
DFP Flashlight with VI and CY LEDs, matching filter glasses, carrying case
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ODFP-VG
DFP Flashlight with VI and GR LEDs, matching filter glasses, carrying case
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ODFP-VR
DFP Flashlight with VI and RB LEDs, matching filter glasses, carrying case
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NIGHTSEA BlueStar
NIGHTSEA BlueStar
NIGHTSEA BLUESTAR™ FLASHLIGHT

The BlueStar flashlight combined with the matched barrier filter glasses is a convenient, powerful tool for exciting fluorescence in many subjects.

The BlueStar uses the latest in high intensity LED technology combined with specialized optics to produce an ultra-tight, ultra-bright 10-degree beam pattern for maximum excitation of fluorescence.

Comes with adjustable, cushioned wrist lanyard, rubber glare guard. Made in USA.


Specifications

Bulb TypeHigh intensity 1W LED Royal Blue - 440 - 460nm
Burn Time6 hours at full power
Lamp Life>10,000 hours
Battery3 C-cell
NIGHTSEA Barrier Filter Glasses
NIGHTSEA Barrier Filter Glasses
NIGHTSEA™ BARRIER FILTER GLASSES

Styles 1 and 2 fit over eyeglasses, Style 3 does not.

Glasses meet ANSI Z87.1 impact standards for safety glasses.

OBLS-1
BlueStar light plus style VG1 filter glasses
AUD461.00
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OBLS-2
BlueStar light plus style VG2 filter glasses
AUD404.00
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OBLS-3
BlueStar light plus style VG3 filter glasses
AUD411.00
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ORG-2
Red filter glasses, style 2
AUD128.00
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OVG-1
Yellow filter glasses, style 1
AUD98.00
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OVG-2
Yellow filter glasses, style 2
AUD121.00
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OVG-3
Yellow filter glasses, style 3
AUD128.00
BACKORDER 
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