Latex flow cytometry particles 0.1%

SKU: SL-001S   |   Availability ⓘ

Latex flow cytometry particles 0.1%

SKU: SL-001S   |   Availability ⓘ
Title
Size
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AU $82.00 Regular price

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Polystyrene latex spheres have been used to calibrate electron microscopes since 1949 when a batch of uniform-sized particles was discovered by Backus and Williams at Dow Chemical. Since then, many graded sizes have been produced and these find a wide variety of applications in laboratories. Adding a tiny amount of the working solution to the specimen surface makes accurate sizing and measurements possible. This is quite useful for all types of microscopy and especially so in SEM when the specimen is not flat and large size errors are possible because of perspective and varying working distances. It is also possible to make up a mixed size solution and this may be employed for comparative measurements of larger and smaller structures.

ProSciTech has increased the number of calibrated latex spheres available to twelve sizes; they range between 0.1 and 15um. They are useful not only for TEM but also for SEM and light microscopy applications. Cost of latex spheres is now quite low, so there is no reason for any lab not to keep suitable calibration spheres at hand.

Latex particles are especially suited for calibrating SEM and TEM magnifications. If several particles are measured at a magnification where the particles are large enough to make an accurate measurement, it can be assumed that the average particle is within 1% of the nominal magnification. Latex particles are fairly inert and do not distort under vacuum or other normal operating conditions.
Latex particles are also used as an internal magnification standard and for estimating particle concentrations by combining equal size drops of latex particles and of a specimen solution.

It is possible to calculate the approximate number of latex particles in the supplied solution using below data or the link to the Spherotech information, which is applicable to any such latex spheres. However, greater precision in determining the particle numbers present is achievable if an electronic particle counter is used.

ADJUSTING PARTICLE NUMBERS TO REQUIRED CONCENTRATIONS
Usually the supplied concentration is too great and requires dilution with high purity water to make a working solution. In microscopy for a given application the number of particles used, regardless of size, needs to be fairly uniform. The number of particles in a given weight/volume solution varies dramatically with particle size. These numbers are given in column two (in the table below) and relate to a 0.1% solution. For a 1% solution its simply x10 and for a 5% solution x50. Column three gives the dilution required to achieve 3.6x10e-6 particles /mL for various latex particle sizes.

Diameter (um) Number of particles/ml 3.6x10e-6/ml
0.1um 1818 x 10e9 ~3.6 x 10e-6 %
0.5um 14.5 x 10e9 ~4.6 x 10e-4 %
1.0um 1.8 x 10e9 ~3.7 x 10e-3 %
0.3um 67 x 10e9 ~1 x 10e-4 %
3.0um 67 x 10e6 ~0.1 %
5.0um 14,5 x 10e6 ~0.46%
10um 1.8 x 10e6 ~3.6%
15um 0.5 x 10e6 ~12.4%

Particle concentration of latex calibration spheres for microscopy are supplied as 0.1% weight by volume within an aqueous solution. Specific gravity of spheres is 1.05. The solution contains an antibacterial agent.
Nine different particle sizes are available. Each tube of the "small" and "medium" sizes contains 5mL. Larger spheres are more expensive and different volumes are used for these. Lids are colour coded contamination between different sizes. Sizes are nominal and the actual size is indicated on the vial.

For a large range of SPHEROTECH special application latex particles see page Q1

LATEX SPHERES FOR MICROSCOPY
Available in the these sizes also.

DOCUMENTS:  

SDS 

Technical Sheet 

Updated: 18-07-2024
Code Title Size Pack Size Availability* Price (ex GST)
SL-001S Default Title 0.1um 5ml In Stock AU $82.00
SL-005S Default Title 0.5um 5ml In Stock AU $82.00
SL-008S Default Title 0.8um 5ml In Stock AU $79.00
SL-020M Default Title 2.0um 5ml 2 weeks AU $80.00
SL-030M Default Title 3.0um 5ml In Stock AU $80.00
SL-050M Default Title 5.0um 5ml 3 in stock AU $80.00
SL-080L Default Title 8.0um 4ml In Stock AU $92.00
SL-100L Default Title 10.0um 3ml In Stock AU $88.00
SL-150L Default Title 15.0um 2.5ml 3 in stock AU $83.00

* Availability Explanation

X weeks – the estimated lead time, based on the average time taken previously to fill orders from this particular supplier. Lead times are from when we receive your order until when we fill your order. For delivery times, please see shipping information below.

In Stock – Plenty of stock on the shelf, ready to ship.

No ETA – our supplier has advised they cannot provide an accurate ETA. Generally there is some issue with supply, and it may be more than 6 months.

REFUNDS AND RETURNS

Please ask prior to returning any goods.

Defective products should be returned to us for replacement or credit. It is best to contact support with as much detail as possible, including pictures, so we can quickly resolve the issue. ProSciTech Pty Ltd complies with the ACCC guidelines, available here: https://www.accc.gov.au/consumers/consumer-rights-guarantees/repair-replace-refund 

If you decide the item purchased does not suit your requirements, you may contact us to see if a return is possible. Some items may not be refunded, so please ask before purchase if you think a return may be necessary. The greater of $15 or 15% re-stocking charge  will be levied on materials returned due to an error on the part of the customer. We try to keep this charge minimal, however returns do have a cost to us.

SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200. 

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

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Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $20
Express shipping $30

Delivery times:

Check your email for tracking details provided at time of shipping for updates of your parcel. We cannot guarantee delivery times, as this is outside of our control. The following are the delivery estimates as given by our couriers. 

Regular shipping
Queensland   1-3 business days 
Sydney   2-5 business days 

Melbourne, Adelaide, Hobart or Canberra 

 3-10 business days 

WA, NT and non-metro areas

 4-10 business days 
Express shipping
Australia wide   1-2 business days

Urgent Orders:

Please request "Express" if you would like us to upgrade your shipment to express delivery. Providing your order fits in a satchel and/or is under 1kg in weight, we will ship your order either DHL, TNT or Express Post. Larger or heavy orders need a quotation for additional freight costs.

Dangerous goods:

See the table on the page for DG shipment costs by road. Please specify when ordering if you require air service (this can be prohibitively expensive). Please request a quotation. We will charge at cost.
 

Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping. 

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

GUARANTEE

All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods. 

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted) 

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products. 

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