{"title":"CHEM BSA, Gelatin \u0026 Sera Reagents","description":"","products":[{"product_id":"aurion-cold-water-fish-skin-gelatin-40","title":"Aurion CFWS gelatin","description":"\u003cp\u003eAurion’s Bovine Serum Albumin is obtained from healthy livestock. BSA should be dissolved in an appropriate buffer, such as phosphate buffered saline, taking care not to denature the protein by foaming. The addition of BSA may cause a drop in pH of the final solution and correction may be required. As a preservative the use of NaN\u003csub\u003e3\u003c\/sub\u003e or Kathon CG is recommended.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eSpecially selected compounds\u003c\/li\u003e\n\u003cli\u003eFor immunohisto and -cytochemistry\u003c\/li\u003e\n\u003cli\u003eFor immunogold detection systems\u003c\/li\u003e\n\u003cli\u003eEnzyme based detection systems\u003c\/li\u003e\n\u003cli\u003eFluorescent detection systems\u003c\/li\u003e\n\u003cli\u003eFor EM and LM specimens\u003c\/li\u003e\n\u003cli\u003eFor bio assays\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThe use of Cold Water Fish Skin Gelatin to prevent background reactions has been recommended by e.g. Behnke et al. (J. Cell Biol. 41, [1986], 386). The product is supplied as a liquid concentrate (40%).\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/proscitech.com.au\/products\/tween-20?variant=42664102101182\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eTween-20\u003c\/a\u003e is a non-ionic detergent with a molecular weight of about 600 and a critical micelle concentration (CMC) of 0.06-0.07% in water at room temperature. Its working mechanism may in part be based on its action as a detergent, binding to the hydrophobic moieties of water insoluble compounds, rendering them hydrophilic. In addition, immuno-compounds may become incorporated into micelles when the Tween-20 concentration is higher than the CMC, for instance at 0.1 % in PBS at pH 7.4.\u003c\/p\u003e\u003cp\u003eNormal sera are used to counteract the non-specific interaction between the sample and immunoglobulins. They can be added to the blocking solution and the incubation media.\u003cbr\u003eAs a rule the normal serum species should be the same as the secondary antibody species (e.g. use normal goat serum with goat-anti-rabbit conjugates).\u003c\/p\u003e\u003cp\u003eNote: normal sera should not be used in combination with Protein A and Protein G gold reagents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStore refrigerated 4-8°C\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25560.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"10ml","offer_id":42664102035646,"sku":"JA900-033","price":124.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/JA900-033.jpg?v=1769570149"},{"product_id":"tween-20","title":"Tween-20 detergent","description":"\u003cp\u003eTween-20 is a non-ionic detergent with a molecular weight of about 600 and a critical micelle concentration (CMC) of 0.06-0.07% in water at room temperature. Its working mechanism may in part be based on its action as a detergent, binding to the hydrophobic moieties of water insoluble compounds, rendering them hydrophilic. In addition, immuno-compounds may become incorporated into micelles when the Tween-20 concentration is higher than the CMC, for instance at 0.1 % in PBS at pH 7.4.\u003c\/p\u003e\u003cp\u003eMany compounds have been added empirically to immunolabeling solutions for the purpose of minimising background staining. Based on years of experience and controlled testing, Aurion has selected a group of compounds that are proven to be the most effective in their background reduction action. Researchers can obtain these items separately from Aurion and prepare blocking and incubation media to suit the needs of their own protocols.\u003c\/p\u003e\u003cp\u003eThe ready-to-use Blocking Solutions and the incubation media additive BSA-c are tuned for optimum background prevention and signal-to-noise ratio. In-depth information can be found in the respective product data sheets.\u003c\/p\u003e\u003cp\u003eAURION also offers a number of components that allow researchers to formulate blocking, incubation and wash solutions according to the needs of their own experiments.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eThe products should be stored at 4-8°C.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eFreezing is not recommended.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/aurion.nl\/wp-content\/uploads\/2016\/12\/MSDS-Tween%EF%A3%A8-20-03.pdf\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"10ml","offer_id":42664102101182,"sku":"JA900-044","price":62.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/EMS25564-T20.png?v=1763964871"},{"product_id":"aurion-normal-serum","title":"Aurion normal serum","description":"\u003cp\u003eAurion’s Bovine Serum Albumin is obtained from healthy livestock. BSA should be dissolved in an appropriate buffer, such as phosphate buffered saline, taking care not to denature the protein by foaming. The addition of BSA may cause a drop in pH of the final solution and correction may be required. As a preservative the use of NaN\u003csub\u003e3\u003c\/sub\u003e or Kathon CG is recommended.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eSpecially selected compounds\u003c\/li\u003e\n\u003cli\u003eFor immunohisto and -cytochemistry\u003c\/li\u003e\n\u003cli\u003eFor immunogold detection systems\u003c\/li\u003e\n\u003cli\u003eEnzyme based detection systems\u003c\/li\u003e\n\u003cli\u003eFluorescent detection systems\u003c\/li\u003e\n\u003cli\u003eFor EM and LM specimens\u003c\/li\u003e\n\u003cli\u003eFor bio assays\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThe use of Cold Water Fish Skin Gelatin to prevent background reactions has been recommended by e.g. Behnke et al. (J. Cell Biol. 41, [1986], 386). The product is supplied as a liquid concentrate (40%).\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/proscitech.com.au\/products\/tween-20?variant=42664102101182\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eTween-20\u003c\/a\u003e is a non-ionic detergent with a molecular weight of about 600 and a critical micelle concentration (CMC) of 0.06-0.07% in water at room temperature. Its working mechanism may in part be based on its action as a detergent, binding to the hydrophobic moieties of water insoluble compounds, rendering them hydrophilic. In addition, immuno-compounds may become incorporated into micelles when the Tween-20 concentration is higher than the CMC, for instance at 0.1 % in PBS at pH 7.4.\u003c\/p\u003e\u003cp\u003eNormal sera are used to counteract the non-specific interaction between the sample and immunoglobulins. They can be added to the blocking solution and the incubation media.\u003cbr\u003eAs a rule the normal serum species should be the same as the secondary antibody species (e.g. use normal goat serum with goat-anti-rabbit conjugates).\u003c\/p\u003e\u003cp\u003eNote: normal sera should not be used in combination with Protein A and Protein G gold reagents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStore refrigerated 4-8°C\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25568.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"Rabbit \/ 5ml","offer_id":42664382496958,"sku":"EMS25568","price":291.0,"currency_code":"AUD","in_stock":true},{"title":"Goat \/ 5ml","offer_id":42664382529726,"sku":"EMS25570","price":210.0,"currency_code":"AUD","in_stock":true},{"title":"Donkey \/ 5ml","offer_id":42664382562494,"sku":"EMS25572","price":277.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/EMS25568-NS.jpg?v=1763504630"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/collections\/Blocking-reagents-overview.jpg?v=1772662524","url":"https:\/\/proscitech.com.au\/collections\/chem-bsa-gelatin-sera-reagents.oembed","provider":"ProSciTech","version":"1.0","type":"link"}