{"title":"CHEM Immunohistochemistry (IHC)","description":"","products":[{"product_id":"citrate-buffer-0-2m","title":"Citrate Buffer 0.2M","description":"\u003cp\u003eCitric acid and Sodium citrate 0.2M\u003c\/p\u003e\u003cp\u003eKeep cold - DO NOT FREEZE\u003c\/p\u003e\u003cp\u003epH 4.0 \u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003epH6.0 - \u003ca class=\"btn\" href=\"https:\/\/laboratoryresource.com\/?navaction=sds\u0026amp;id=359\" rel=\"noopener noreferrer\" style=\"font-size: 16px; display: inline-block; width: 250px; text-align: center; padding: 10px; color: rgb(255, 255, 255); background-color: rgb(0, 128, 0); text-decoration-line: none; border-radius: 5px; box-shadow: rgba(0, 0, 0, 0.2) 0px 4px 6px; background-image: linear-gradient(rgb(0, 128, 85), rgb(11, 128, 0)); font-family: helvetica neue; font-weight: bold; transition: transform 0.1s;\" target=\"_blank\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"4.0 \/ 1L","offer_id":42664336228542,"sku":"EMS11492-04","price":167.0,"currency_code":"AUD","in_stock":true},{"title":"6.0 \/ 1L","offer_id":42664336261310,"sku":"EMS11492-06","price":171.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_c21cef0a-bb67-4f04-bbba-8c3ea39d00a4.jpg?v=1763504271"},{"product_id":"universal-antibody-dilution-buffer","title":"Universal antibody dilution buffer","description":"\u003cp\u003eThis ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eReagent Supplied\u003c\/strong\u003e: Ready to use buffer\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"100ml","offer_id":42664383578302,"sku":"EMS25885-01","price":162.0,"currency_code":"AUD","in_stock":true},{"title":"500ml","offer_id":42664383611070,"sku":"EMS25885-05","price":414.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_0a8b7335-0468-4800-9d6f-be70f42418e3.jpg?v=1763504639"},{"product_id":"antibody-dilution-buffer-free-bsa","title":"Antibody diluent, free BSA","description":"\u003cp\u003eThis ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food colour.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eReagent Supplied\u003c\/strong\u003e: Ready to use buffer\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/25886.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"100ml","offer_id":42665295675582,"sku":"EMS25886-01","price":216.0,"currency_code":"AUD","in_stock":true},{"title":"500ml","offer_id":42665295708350,"sku":"EMS25886-05","price":444.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_d0a221e2-a241-45e2-a3b8-dfaa0239fddd.jpg?v=1769570226"},{"product_id":"antibody-dilution-buffer","title":"Antibody diluent","description":"\u003cp\u003eUse a buffer to dilute your primary and secondary antibodies, particularly if they have been stored for an extended period, even at -20°C in glycerol or in a refrigerator.. Nonspecific binding of the antibodies, negative effects of disturbing substances, and low or medium affinity cross-reactivities of the antibodies will be minimised, making your result more reliable. Ideal for IHC on frozen and formalin-fixed sections, flow cytometry on fixed cells, Western Blot, and other immune assays.\u003c\/p\u003e\u003cp\u003eWhen used in pathology, it also greatly reduces the non-specific reactivity of human serum components and immunoglobulins in tissue, vessels, and cells with mouse antibodies used on section.\u003c\/p\u003e\u003cp\u003eFor especially \"trouble\"-giving antibodies, as well as for in situ PCR applications, this diluent may also be used as a washing buffer, preventing secondary binding of your analysts during washing.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cem\u003eStore refrigerated 2-8 deg C\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003ca class=\"btn\" href=\"https:\/\/proscitech.freshdesk.com\/en\/support\/solutions\/articles\/44002566212-antibody-diluent\" rel=\"noopener noreferrer\" style=\" font-size: 16px; display: inline-block; width: 160px; text-align: center; padding: 10px; color: #000000; background-color: #fff; text-decoration: none; border-radius: 5px; box-shadow: 0px 4px 6px rgba(0, 0, 0, 0.2); background-image: linear-gradient(to bottom, #007bff; font-family: helvetica neue; transition: transform 0.1s ease;\" target=\"_blank\"\u003eTechnical Data\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/62713.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aptum Biologics","offers":[{"title":"50ml","offer_id":42665295773886,"sku":"EMS62713","price":371.0,"currency_code":"AUD","in_stock":true},{"title":"125ml","offer_id":42665295806654,"sku":"EMS62714","price":660.0,"currency_code":"AUD","in_stock":true},{"title":"500ml","offer_id":42665295839422,"sku":"EMS62715","price":2131.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/Antibody_20dilution.jpg?v=1763524843"},{"product_id":"phem-buffer","title":"PHEM buffer","description":"\u003cp\u003ePHEM buffer has mostly been utilised to treat tissues and cell cultures for immunocytochemical research.  Most antigens, particularly intracellular antigens, stain better in PHEM than in PBS; it also has a more restricted influence on metabolic processes and enzymes. The preservation of cellular ultrastructure, particularly microtubules, is another advantage of using PHEM.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eConveniently packaged.\u003c\/li\u003e\n\u003cli\u003ePrepared fresh for each order.\u003c\/li\u003e\n\u003cli\u003eEconomical and time-saving.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eStore refrigerated 2-8°C\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e0.1M - \u003ca class=\"btn\" href=\"https:\/\/laboratoryresource.com\/?navaction=sds\u0026amp;id=680\" rel=\"noopener noreferrer\" style=\"font-size: 16px; display: inline-block; width: 250px; text-align: center; padding: 10px; color: rgb(255, 255, 255); background-color: rgb(0, 128, 0); text-decoration-line: none; border-radius: 5px; box-shadow: rgba(0, 0, 0, 0.2) 0px 4px 6px; background-image: linear-gradient(rgb(0, 128, 85), rgb(11, 128, 0)); font-family: helvetica neue; font-weight: bold; transition: transform 0.1s;\" target=\"_blank\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e0.2M - \u003ca class=\"btn\" href=\"https:\/\/laboratoryresource.com\/?navaction=sds\u0026amp;id=681\" rel=\"noopener noreferrer\" style=\"font-size: 16px; display: inline-block; width: 250px; text-align: center; padding: 10px; color: rgb(255, 255, 255); background-color: rgb(0, 128, 0); text-decoration-line: none; border-radius: 5px; box-shadow: rgba(0, 0, 0, 0.2) 0px 4px 6px; background-image: linear-gradient(rgb(0, 128, 85), rgb(11, 128, 0)); font-family: helvetica neue; font-weight: bold; transition: transform 0.1s;\" target=\"_blank\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/11162.pdf\" rel=\"noreferrer noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"0.1M \/ 225ml","offer_id":43210330636478,"sku":"EMS11162","price":139.0,"currency_code":"AUD","in_stock":true},{"title":"0.1M \/ 500ml","offer_id":43210330669246,"sku":"EMS11163","price":222.0,"currency_code":"AUD","in_stock":true},{"title":"0.2M \/ 225ml","offer_id":43210330702014,"sku":"EMS11164","price":139.0,"currency_code":"AUD","in_stock":true},{"title":"0.2M \/ 500ml","offer_id":43210330734782,"sku":"EMS11165","price":220.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/EMS11162.jpg?v=1748487780"},{"product_id":"citrate-buffer-0-1m","title":"Citrate buffer 0.1M","description":"\u003cp\u003eCitric acid and Sodium citrate 0.1M\u003cbr\u003epH range 4.6-6.2\u003c\/p\u003e\u003cp\u003eKeep cold - DO NOT FREEZE\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"4.6-6.2 \/ 225ml","offer_id":43319914889406,"sku":"EMS11490","price":66.0,"currency_code":"AUD","in_stock":true},{"title":"4.6-6.2 \/ 500ml","offer_id":43319914922174,"sku":"EMS11492","price":88.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/SmallBottle_b2e7f471-1c88-4fa0-8e9f-fb8e891a68e0.jpg?v=1748487865"},{"product_id":"tris-buffer-0-2m","title":"Tris buffer 0.2M","description":"\u003cp\u003eAll of our ready-to-use buffers are:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eConveniently packaged.\u003c\/li\u003e\n\u003cli\u003ePrepared fresh for each order.\u003c\/li\u003e\n\u003cli\u003eEconomical and time-saving.\u003c\/li\u003e\n\u003cli\u003eDesired pH should be specified when ordering.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eThe buffer is a vehicle for the fixative and requires certain properties:\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eTo maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.\u003c\/li\u003e\n\u003cli\u003eThe ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"8 \/ 250ml","offer_id":43319945953470,"sku":"EMS11730-05","price":75.0,"currency_code":"AUD","in_stock":true},{"title":"8 \/ 500ml","offer_id":43319945986238,"sku":"EMS11730-06","price":105.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_195d83c2-2b4c-4d0b-b27e-299f91bc18b5.jpg?v=1748487882"},{"product_id":"tris-buffered-saline-ph-7-4","title":"Tris buffered saline, pH 7.4","description":"\u003cp\u003ePrepared buffer\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"7.4 \/ 250ml","offer_id":43319946019006,"sku":"EMS11730-08","price":75.0,"currency_code":"AUD","in_stock":true},{"title":"7.4 \/ 500ml","offer_id":43319946051774,"sku":"EMS11730-09","price":105.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_d5c1bf0c-07f2-4a60-81cc-3a7207b70bfc.jpg?v=1748487884"},{"product_id":"citrate-buffer","title":"Citrate buffer","description":"\u003cp\u003eThis buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e 2-8°C. \u003cstrong\u003eDO NOT FREEZE \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eIntended Use: \u003c\/strong\u003e1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.\u003cbr\u003e\u003cstrong\u003eReagent:\u003c\/strong\u003e 10x, pH 6 antigen retriever solution. \u003cstrong\u003e\u003cbr\u003ePreparation of Working Solution: \u003c\/strong\u003eDilute this 10x buffer as needed (e.g. 90ml of deionised or distilled water + 10ml of this buffer), mix well, 1x buffer can be stored at 2-8°C. 2\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"6 \/ 100ml","offer_id":43331115221182,"sku":"EMS64142-07","price":165.0,"currency_code":"AUD","in_stock":true},{"title":"6 \/ 1L","offer_id":43331115253950,"sku":"EMS64142-08","price":305.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_01983f67-0915-4a80-9b54-9a5dcc05513c.jpg?v=1748487919"},{"product_id":"edta-buffer-10x","title":"EDTA buffer (10X)","description":"\u003cp\u003eThis buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e 2-8°C. \u003cstrong\u003eDO NOT FREEZE \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eIntended Use: \u003c\/strong\u003e1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.\u003cbr\u003e\u003cstrong\u003eReagent:\u003c\/strong\u003e 10x, pH 8.5 antigen retriever solution.\u003cbr\u003e\u003cstrong\u003ePreparation of Working Solution: \u003c\/strong\u003eDilute this 10x buffer as needed (e.g. 90ml of deionised or distilled water + 10ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"8.5 \/ 100ml","offer_id":43331115286718,"sku":"EMS64142-09","price":165.0,"currency_code":"AUD","in_stock":true},{"title":"8.5 \/ 1L","offer_id":43331115319486,"sku":"EMS64142-10","price":303.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_0e467567-8f3f-4218-9f0c-0f83d1f03594.jpg?v=1748487921"},{"product_id":"r-universal-epitope-recovery-buffer","title":"R-Universal epitope recovery buffer","description":"\u003cp\u003eR-Universal Buffer utilises a novel (patent-pending) system for the recovery of epitopes, largely based on altering the fixing effects of formaldehyde, which also has generated links primarily between ε-amino lysine groups and several other amino groups.\u003c\/p\u003e\u003cp\u003eR-Universal Buffer could also be used in any heat-induced epitope recovery system, but has been specifically adapted for the treatment of tissue sections in the EMS Antigen Retriever (62700 Series). Using this combination guarantees the highest success rate in the recovery of epitope for any antibody, particularly one which has never been previously used in formalin-fixed sections.\u003c\/p\u003e\u003cp\u003eThe buffer has been widely studied in United Kingdom's pathology departments and has already shown fantastic results when used with various antibodies, including those typically requiring treatment only in low, or even high pH buffers for efficient staining, or requiring protolithic treatment.\u003c\/p\u003e\u003cp\u003eEpitope recovery on formalin-fixed, paraffin-embodied tissue sections requires heat-induced buffer treatment or, occasionally, proteolitic treatment of the deparaffinised tissue section.\u003c\/p\u003e\u003cp\u003eWhich buffer to use depends largely on the specific antibody and the properties of the recognised epitope. Considerable research and practise exists on the use of many buffers, including Citrate pH 3.4, Citrate 6.0, EDTA 8.0, Tris 9.0-10.0, Tris-EDTA, etc.\u003c\/p\u003e\u003cp\u003eFor individual antigens, the time of recovery in the individual buffer should be defined, as this may be different, and the treatment often destroys the epitope.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProperties \u003c\/strong\u003eClear, non-toxic solution.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePresentation: \u003c\/strong\u003eR-Universal Buffer is supplied as 10x concentrate. For epitope recovery dilute 1 part of stock with 9 parts of deionised water.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication: E\u003c\/strong\u003epitope recovery dilute 1 part of stock with 9 parts of deionised water.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStability and Storage: \u003c\/strong\u003ePreparation is stable for 1 year when stored unopened at +40°C. Every lot is issued with a certificate indicating the expiry date.\u003c\/p\u003e\u003cp\u003eAfter opening, store at +40°C in the refrigerator and use within 6 months.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCertification: \u003c\/strong\u003eEach lot is certified for compliance to specifications. The product is produced under DIN EN ISO 9001 :2008 Quality Management system for the products in Immunoassay Development and Measurement, Products for Bioanalytics and lmmunoassays.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aptum Biologics","offers":[{"title":"125ml","offer_id":43579377582270,"sku":"EMS62719-10","price":433.0,"currency_code":"AUD","in_stock":true},{"title":"500ml","offer_id":43579377615038,"sku":"EMS62719-20","price":660.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/EMS62719-10.jpg?v=1748751649"},{"product_id":"antibody-diluent-for-frozen-sections","title":"Antibody diluent for frozen sections","description":"\u003cp\u003eBuffer for diluting your primary and secondary antibodies, especially if they were stored for a while, even at -20°C in glycerol, or in refrigerator. Nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies will be minimised, making your result more reliable. Excellent for IHC (frozen and formalin sections), flow cytometry on fixed cells, Western Blot and other immune assays.\u003c\/p\u003e\u003cp\u003eWhen used in pathology, in also greatly reduces non-specific reactivity of human serum components and immunoglobulins in tissue, vessels and cells with mouse antibodies used on section.\u003c\/p\u003e\u003cp\u003eFor especially \"trouble\"-giving antibodies, as well as for in situ PCR applications, this diluent may also be used as a washing buffer, preventing secondary binding of your analystes during washing.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cem\u003eStore refrigerated 2-8 deg C\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/62713.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aptum Biologics","offers":[{"title":"50ml","offer_id":44863076434110,"sku":"EMS62713-01","price":343.0,"currency_code":"AUD","in_stock":true},{"title":"125ml","offer_id":44863076466878,"sku":"EMS62714-01","price":456.0,"currency_code":"AUD","in_stock":true},{"title":"500ml","offer_id":44863076499646,"sku":"EMS62715-01","price":2134.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/EMS62713-B.jpg?v=1748752135"},{"product_id":"hrp-conjugate-diluent","title":"HRP conjugate diluent","description":"\u003cp\u003eSpecifically designed for preparing solution of your HRP-conjugate used as the detection reagent.\u003c\/p\u003e\u003cp\u003eThis is the antibody diluent buffer, which contains an additional component to help stabilize your HRP conjugate.  Enables further standardization of the assay by preparing ready-to-use conjugate solutions in advance, which can be stored in the refrigerator without loss of activity.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cem\u003eKeep cold - DO NOT FREEZE\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003ca class=\"btn\" href=\"https:\/\/proscitech.freshdesk.com\/en\/support\/solutions\/articles\/44002566213-antibody-diluent-frozen-sections\" rel=\"noopener noreferrer\" style=\" font-size: 16px; display: inline-block; width: 160px; text-align: center; padding: 10px; color: #000000; background-color: #fff; text-decoration: none; border-radius: 5px; box-shadow: 0px 4px 6px rgba(0, 0, 0, 0.2); background-image: linear-gradient(to bottom, #007bff; font-family: helvetica neue; transition: transform 0.1s ease;\" target=\"_blank\"\u003eTechnical Data\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/microscopy\/technical\/msds\/62716.pdf\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"50ml","offer_id":44863076565182,"sku":"EMS62716","price":358.0,"currency_code":"AUD","in_stock":true},{"title":"125ml","offer_id":44863076597950,"sku":"EMS62717","price":756.0,"currency_code":"AUD","in_stock":true},{"title":"500ml","offer_id":44863076630718,"sku":"EMS62718","price":2199.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/EMS62716.jpg?v=1748752136"},{"product_id":"section-block-solutions","title":"Section block solutions","description":"\u003cp\u003eA new class of blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates you use to charged surface of the slide and tissue section. Greatly reduces non-specific binding while preserving the specific reaction, by saturating potential non-specific protein-protein interactions. Moreover, in contrast to BSA-based, IgGm casein or serum-based blocking solutions there is no interaction of specific antibody and blocking protein itself. Is not comparable to other commercially available or home-made blocking solutions. \u003c\/p\u003e\u003cp\u003eRecommended for research and diagnostic pathology, especially for retrieved sections and polyclonal antibodies.\u003c\/p\u003e","brand":"Aptum Biologics","offers":[{"title":"50ml","offer_id":45343556960446,"sku":"EMS62710","price":288.0,"currency_code":"AUD","in_stock":true},{"title":"125ml","offer_id":45343556993214,"sku":"EMS62711","price":579.0,"currency_code":"AUD","in_stock":true},{"title":"500ml","offer_id":45343557025982,"sku":"EMS62712","price":1972.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/EMS62710.png?v=1756347967"},{"product_id":"tris-buffer-10x","title":"Tris buffer, 10x","description":"\u003cp\u003eThis buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage: 2-8°C. DO NOT FREEZE \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eIntended Use: \u003c\/strong\u003e1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.\u003cbr\u003e\u003cstrong\u003eReagent:\u003c\/strong\u003e 10x, pH 10 antigen retriever solution. \u003cstrong\u003e\u003cbr\u003ePreparation of Working Solution: \u003c\/strong\u003eDilute this 10x buffer as needed (e.g. 90ml of deionised or distilled water + 10ml of this buffer), mix well.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/71302.pdf\" rel=\"noopener noreferrer\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"10 \/ 100ml","offer_id":45344004079806,"sku":"EMS64142-11","price":167.0,"currency_code":"AUD","in_stock":true},{"title":"10 \/ 1L","offer_id":45344004112574,"sku":"EMS64142-12","price":301.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_9406bdb7-8207-4362-a815-71a86f97118c.jpg?v=1756360596"},{"product_id":"antigen-preserve-solution-vii","title":"Antigen preserve solution VII","description":"\u003cp\u003eThis ready-to-use antibody dilution buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin-free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food coloring.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eReagent Supplied\u003c\/strong\u003e: Ready-to-use buffer\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e: Dilute antibodies, primary or secondary, as recommended in the protocol by the manufacturer of these products.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e: Store refrigerated at 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of the antibodies.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/1227SK.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"1000ml","offer_id":45354730717374,"sku":"EMS1227-SK","price":373.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/ChemBottle_d3d00332-6187-4b77-8cc3-0223db61af4b.jpg?v=1763965371"},{"product_id":"3-3-diaminobenzidine-dab-em-grade-purified-free-base-dg","title":"3,3'-Diaminobenzidine (DAB), EM grade, purified free-base (DG)","description":"\u003cp\u003eUsed as an ultrastructural protein tracer for immuno-cytochemistry. A possible carcinogen.\u003c\/p\u003e\u003cp\u003e(3,3',4,4'-Biphenyltetramin)\u003cbr\u003eC\u003csub\u003e12\u003c\/sub\u003eH\u003csub\u003e14\u003c\/sub\u003eN\u003csub\u003e4\u003c\/sub\u003e   \u003c\/p\u003e\u003cp\u003eF.W. 214.27   \u003c\/p\u003e\u003cp\u003eCAS #91-95-2\u003c\/p\u003e\u003cp\u003eMelting point: 173°C\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/13080.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Aurion","offers":[{"title":"1g","offer_id":45354743365822,"sku":"EMS13060","price":47.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/SmallPowderBottle_9a662063-f706-4150-8a47-4a8906fefc65.jpg?v=1756701030"},{"product_id":"3-3-5-5-tetramethyl-benzidine-tmb-dg","title":"3,3,5,5-tetramethyl benzidine (TMB) (DG)","description":"\u003cp\u003eA non-carcinogenic substitute for DAB and DAB-HCl. Used as sensitive and specific reagent for detection of blood.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/13080.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"1g","offer_id":45354743398590,"sku":"EMS13070","price":100.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/SmallPowderBottle_8fc7727e-3ef7-4934-825a-6429226d5e65.jpg?v=1762233773"},{"product_id":"dab-hcl-dg","title":"DAB-HCL (DG)","description":"\u003cp\u003eUsed for histochemical localisation of peroxidase with DAB and in immunocytochemistry. Sternberger, et.al., J. Histochem, Cytochem., 18,215 (1970)\u003c\/p\u003e\u003cp\u003eC\u003csub\u003e12\u003c\/sub\u003eH\u003csub\u003e14\u003c\/sub\u003eN\u003csub\u003e4\u003c\/sub\u003e·4HCl   \u003c\/p\u003e\u003cp\u003eF.W. 360.12   \u003c\/p\u003e\u003cp\u003eCAS #7411-49-6\u003c\/p\u003e\u003cp\u003eMelting point: 300°C\u003c\/p\u003e\u003cp\u003eWe are now offering DAB 4HCl pre-weighed and packed in tablet form, to facilitate your work. Each tablet contains 10mg. Just break the tablet or tablets in a suitable bottle and add your buffer. All weighing and contact with the solid (DAB-HCl) is avoided.\u003c\/p\u003e\u003cp\u003eAvailable in 1g or 10g comes in (pack of 10 or 20)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStore refrigerated 4-8°C\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003ca class=\"btn\" href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/datasheet\/13080\" style=\" font-size: 16px; display: inline-block; width: 160px; text-align: center; padding: 10px; color: #000000; background-color: #fff; text-decoration: none; border-radius: 5px; box-shadow: 0px 4px 6px rgba(0, 0, 0, 0.2); background-image: linear-gradient(to bottom, #007bff; font-family: helvetica neue; transition: transform 0.1s ease;\" target=\"_blank\"\u003eTechnical Data\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.emsdiasum.com\/docs\/technical\/msds\/13080.pdf\" target=\"_blank\" title=\"Open document in new tab\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Electron Microscopy Sciences (EMS)","offers":[{"title":"1g","offer_id":45354743562430,"sku":"EMS13080","price":100.0,"currency_code":"AUD","in_stock":true},{"title":"Pack\/10","offer_id":45354743595198,"sku":"EMS13082","price":263.0,"currency_code":"AUD","in_stock":true},{"title":"Pack\/20","offer_id":45354743627966,"sku":"EMS13083","price":489.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/files\/SmallPowderBottle_ecb83283-e245-4a13-b7d3-dab02e5e6fdc.jpg?v=1756701035"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1784\/2811\/collections\/download_79363187-89c9-4a36-8d51-3401aa129bb6.jpg?v=1773723907","url":"https:\/\/proscitech.com.au\/collections\/chem-immunohistochemistry-ihc.oembed","provider":"ProSciTech","version":"1.0","type":"link"}