INCYTO C-Chip counting chamber

The C-Chip is a disposable plastic haemocytometer used for manual cell counting. It consists of 2 enclosed, surface-patterned chambers with 2 ports for sample injection.The C-Chip counting chambers are particularly suited for phase contrast microscopy, but bright-field viewing is very satisfactory.In a conventional glass haemocytometer, improper fitting of the chamber and coverslip changes the volume of the sample introduced into the chamber. C-Chip’s chamber with integrated coverslip solves the problem.  It is a good standard practice to use a disposable haemocytometer for clinical labs and laboratories dealing with infectious materials. It is safer.Features:Quartz grade optical plasticPrecise design leads to highly reproducible resultsTwo counting chambers fitted with clearest grid pattern rich in contrastExposure to infectious material is reduced due to its closed systemContinuous sample...

The C-Chip is a disposable plastic haemocytometer used for manual cell counting. It consists of 2 enclosed, surface-patterned chambers with 2 ports for sample injection.

The C-Chip counting chambers are particularly suited for phase contrast microscopy, but bright-field viewing is very satisfactory.

In a conventional glass haemocytometer, improper fitting of the chamber and coverslip changes the volume of the sample introduced into the chamber. C-Chip’s chamber with integrated coverslip solves the problem.  It is a good standard practice to use a disposable haemocytometer for clinical labs and laboratories dealing with infectious materials. It is safer.

Features:

  • Quartz grade optical plastic
  • Precise design leads to highly reproducible results
  • Two counting chambers fitted with clearest grid pattern rich in contrast
  • Exposure to infectious material is reduced due to its closed system
  • Continuous sample containment
  • Light and unbreakable compared to glass
  • No need for cover slips
  • Time and cost saving
  • For single use

Neubauer Improved Semen Test Mk II Burker Turk Fuchs-Rosenthal
Outer Size


Width 25mm x Length 75mm x Thickness 1.6mm

Chamber Depth

0.1 mm

 0.02 mm  0.1 mm  0.02 mm
Chamber Volume


10 µl


3 µl  10 µl  20 µl  


 


 


Neubauer Improved


SV520-N and SV521-N, C-Chip disposable haemocytometer, have two precision engineered individual counting chambers (100 mm depth) with integrated cover slips. The chamber has ports for sample loading. Neubauer Improved (NI) grid pattern is embedded in each chamber.  Each grid pattern has nine large squares each measuring 1 x 1 mm and a total counting area of 3 x 3 mm.

 


Applications:


  • Mammalian cell
  • Blood analysis (Hematology): Blood cell counting
  • Cell culture: Cell concentration measurement, Cell viability


 


Calculation:


The full grid on a haemocytometer contains 9 squares, each of which is 1 mm square.  Large cells are counted using the four large corner squares (and the middle one).  If you use a dense suspension of small cells, the four 1/25 sq. mm corners plus the middle square in the central square are used.


The grid consists of 9 large squares, each measuring 1 x 1 mm, and a chamber depth of 0.1 mm.  Each square has a total volume of 0.1 mm3 or 10-4cm3.  The central square is divided into 25 small squares with triple lines and four corner squares are divided into 16 small squares.


Each large square has a surface area of 1.0mm2, and the depth of the chamber is 0.1mm.  As there are 1000 mm3 per ml, each large square represents a volume of 0.0001ml, so that it is equal to 1/ 0.0001ml = 10,000 = (104)


Cells per ml = Average count per square x dilution factor x 10,000.

 


 

Semen Test Mk II

Using a disposable C-Chip hemacytometer for semen analysis allows for clear visualization of the sperm that simply wasn't possible before. C-Chip DHC-S02 is a phase type hemacytometer (using phase contrast microscopy). Light microscopy could also be used for sperm counting, magnification x 200~400.

 

Applications:  

  • Sperm cell counting (bacterial, yeast, sperm)
  • Blood analysis(Hematology): Blood cell counting
  • Cell culture: Cell concentration measurement, Cell viability test
  • Microbiology : Fungal spores counting

 

Calculation:

The DHC-S consists of 100 small squares in the large center square.  Each small square measures 0.1 x 0.1 mm, with a chamber depth of 0.02 mm.  Each small square has a total volume of 2.0 x 10-4mm3 or 2.0 x 10-7cm3.

 Count all the cells in the 100 small squares within the large center square.  The large center square has a surface area of 1.0 mm2 and a chamber depth of 0.02 mm.   The large center square has 0.02 mm3.   As there are 1000mm3 per ml, the center square represents a volume of 0.00002ml, so that equates to 1/ 0.0002ml = 50,000 (50,000 = 1/ (100 squares x 2.0 x 10-7cm3).   The volume factor is then 50,000.

Cells per ml = Number of cells in 100 small squares x dilution factor x 50,000

 


 

Burker Turk

Applications: 

  • Cell counts in cerebrospinal fluid (CSF), rare cells
  • Blood analysis(Hematology): Blood cell counting
  • Cell culture: Cell concentration measurement, Cell viability test
  • Microbiology : Fungal spores counting

 

Calculation:

The full grid on a hemocytometer contains 9 squares, each of which is 1 micro meter (mm) square.  Large cells are counted using the four large corner squares (and the middle one).

For a dense suspension of small calls, the four 1/25 sq. mm corners plus the middle square and the central square are used.

It consists of 9 large squares, each measuring 1 x 1 mm, with the depth of chamber of 0.1 mm.  Each square has a total volume of 0.1 mm3 or 10-4cm3.  The central square is divided into 25 small squares with triple lines and four corner squares divided into 16 small squares.

Each large square has a surface area of 1.0mm2, and a chamber depth of 0.1mm.  There are 1000 mm3 per ml, each large square represents a volume of 0.0001ml, that equates to 1/ 0.0001ml = 10,000 = (104).  The volume factor is 10,000.

 



Fuchs-Rosenthal

Manual microscopic cell counting in a Fuchs-Rosenthal (FR) chamber has been the gold standard for quantification of leukocytes in cerebrospinal fluid (CSF). An excellent correlation between cell counts obtained with the C-Chip chamber and FR chamber was observed. (“Determination of leukocyte counts in cerebrospinal fluid with a disposable plastic hemocytometer”)

C-Chip  eliminates the risk of exposure to potentially infectious materials and the risk of improper fitting of the chamber and the cover slip, which improves the precision and repeatability.

 

Applications: 

  • Cell counts in cerebrospinal fluid (CSF), rare cells

 

Calculation: 

The full grid on a hemocytometer contains 16 squares, each of which is 1 mm square.  A dense suspension of small cells is 1/4 sq. mm.

Each large square has a surface area of 1.0mm2, and the depth of the chamber is 0.2mm.  As there are 1000 mm3 per ml, each large square represents a volume of 0.0002ml, so that it is equal to 1/ 0.0002ml = 5,000. The volume factor is 5,000.

Cells per ml = Average count per square x dilution factor x 5,000

Code Title Style Pack Size Availability Price
SV526-B INCYTO C-Chip counting chamber Burker Turk pack/50 2 weeks AU $118.00
SV527-B INCYTO C-Chip counting chamber Burker Turk pack/20 2 weeks AU $63.00
SV524-F INCYTO C-Chip counting chamber Fuchs-Rosenthal pack/50 2 weeks AU $118.00
SV525-F INCYTO C-Chip counting chamber Fuchs-Rosenthal pack/20 Only 1 AU $63.00
SV520-N INCYTO C-Chip counting chamber Neubauer improved pack/50 In Stock AU $155.00
SV521-N INCYTO C-Chip counting chamber Neubauer improved pack/20 2 weeks AU $80.00
SV522-S INCYTO C-Chip counting chamber Semen Test MkII pack/50 2 weeks AU $198.00
SV523-S INCYTO C-Chip counting chamber Semen Test MkII pack/20 Only 3 AU $96.00
Code Title Style Pack Size Availability Price
SV526-B INCYTO C-Chip counting chamber Burker Turk pack/50 2 weeks AU $118.00
SV527-B INCYTO C-Chip counting chamber Burker Turk pack/20 2 weeks AU $63.00
SV524-F INCYTO C-Chip counting chamber Fuchs-Rosenthal pack/50 2 weeks AU $118.00
SV525-F INCYTO C-Chip counting chamber Fuchs-Rosenthal pack/20 Only 1 AU $63.00
SV520-N INCYTO C-Chip counting chamber Neubauer improved pack/50 In Stock AU $155.00
SV521-N INCYTO C-Chip counting chamber Neubauer improved pack/20 2 weeks AU $80.00
SV522-S INCYTO C-Chip counting chamber Semen Test MkII pack/50 2 weeks AU $198.00
SV523-S INCYTO C-Chip counting chamber Semen Test MkII pack/20 Only 3 AU $96.00