BBI protein A/G, EM-grade
The gold conjugates are made to the highest standards and specifications, yielding excellent results when correctly used. All gold conjugates are supplied in one of the two buffers listed below. Buffer constituents dictate the shelf life and storage conditions for the individual conjugates.
Gold conjugates for electron microscopy (EM) are supplied in the following buffer: 20mM Tris (tris-hydroxymethyl-aminomethane); 20mM sodium azide; 154mM NaCl; 1% BSA; 20% glycerol; pH 8.2.
Recipe to make 100ml: 0.242g (20mM) Tris + 0.9g (154mM) NaCl + ultrapure water to make 100ml. Adjust pH from 7.2 to 8.2 with 1N HCl or 1N NaOH.
Storage: Stable for 1 year at 4 deg. C; stability for 2+ years at –20 deg. C. The conjugates demonstrate remarkable stability at ambient temperatures for up to 7 days. Repeat freezing and thawing is not recommended.
Gold conjugates for lateral flow applications are supplied in the following buffer: 2mM sodium tetraborate at pH 8.2 containing 0.095% sodium azide.
Storage: Stable for 1-2 years at 4-8 deg. C. DO NOT FREEZE. The conjugates demonstrate remarkable stability at ambient temperatures for up to 7 days.
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