EMS 111 platform shaker, specify 110 or 220V

Freeze Substitution is a method to dehydrate and quickly fix frozen cells at low temperatures that normally takes days to finish. With K.L's incredible work, McDonald and R.I. Webb1 has now developed a new technique of freezing with a basic kit that we are proud to offer. With this special kit scientists can now accomplish outstanding freeze substitution results for small volume cells like bacteria and tissue culture cells in as little as 90 minutes.Kit FeaturesEMS 111 Platform ShakerEMS 002 Ice ChestEMS 003 Heater BlockEMS 004 Temperature ProbeEMS 005 Cryo TubesEMS 006 Data LoggerProcess of Freeze SubstitutionCool the metal block by completely submerging in the ice bucket's liquid nitrogen. Leave for 5 minutes or until the "boiling" stops.Transfer samples to cryovials with frozen fixative in a separate box, maintaining it all at temperature levels of liquid nitro...

Freeze Substitution is a method to dehydrate and quickly fix frozen cells at low temperatures that normally takes days to finish. With K.L's incredible work, McDonald and R.I. Webb1 has now developed a new technique of freezing with a basic kit that we are proud to offer. 

With this special kit scientists can now accomplish outstanding freeze substitution results for small volume cells like bacteria and tissue culture cells in as little as 90 minutes.

Kit Features

  • EMS 111 Platform Shaker
  • EMS 002 Ice Chest
  • EMS 003 Heater Block
  • EMS 004 Temperature Probe
  • EMS 005 Cryo Tubes
  • EMS 006 Data Logger

Process of Freeze Substitution

  1. Cool the metal block by completely submerging in the ice bucket's liquid nitrogen. Leave for 5 minutes or until the "boiling" stops.
  2. Transfer samples to cryovials with frozen fixative in a separate box, maintaining it all at temperature levels of liquid nitrogen. Seal firmly and also be sure that now the vial does not include any liquid nitrogen. Trapped liquid can cause the vials to explode upon warming. When sealing the tube it is best to use a room temperature lid.
  3. Put the vials with samples into holes in the cooled metal block.
  4. Go to a PC (Macs won't work) that has the Lascar datalogger software installed and name and start the program.
  5. Pour off the liquid nitrogen from the block and box, making sure not to let the cryovials come out of the holes in the block.
  6. Arrange the block so the cryovials are horizontal and put the tops of the vials against one side of the foam box. Use a piece of foam or wadded up paper behind the block so it keeps the vials from falling out of the block during shaking.
  7. Turn on the shaker at 100-125 rpm and allow it to gradually warm until the temperature is at least 0°C before removing the vials for rinsing and resin infiltration. This operation should take place in a fume hood in case there is any leakage of osmium-acetone from poorly sealed vials. Freeze substitution will take about 2 hours with the lid off, and about 3 hours with the lid on (though this may vary from lab to lab).
  8. Remove the vials from the metal block and place them onto a rocker at room temperature and wait until they come to about 20°C, then stop the datalogger and save the files.
  9. Rinse out the fixative with 3-4 rinses in acetone and proceed to infiltration and embedding. Take care opening the vials as pressure built up inside can cause a spray of the freeze substitution media.

**NOTE: No dry ice is required for this procedure

Code Title Pack Size Availability Price Updated: 20-09-2021
EMS34502 EMS 111 platform shaker, specify 110 or 220V Each 2 weeks AU $1,504.00
Code Title Pack Size Availability Price
EMS34502 EMS 111 platform shaker, specify 110 or 220V Each 2 weeks AU $1,504.00