Cargille was first to introduce PCB-Free immersion oils in 1972. Though all immersion oils are now PCB free, no other immersion oil meets FDA criteria; and Cargille's exceeds ISO, DIN and all instrument manufacturer's standards.
Minimum shelf life of these immersion oils is 5 years and can be twice that.
Blending Oils from the Miscible Group: The Miscible Group of immersion oils is A, B, NVH and OVH. Users can easily blend any two immersion oils from the Miscible Group to obtain an immersion oil with an intermediate viscosity while maintaining the optical properties common to both. Type 37 can be blended with type B.
All Cargille immersion oils are classed as low fluorescence, however, for work with weak fluorescence, types FF and LDF - see below - are required.
50mL is supplied in a high density polyethylene bottle with a pointed, twist closure suited to place single drops onto slides. For refills and large users these oils are available in larger glass bottles and in the original 1 US gallon (3.785 litre) steel cans with resealable pouring spout. We stock the gallon container for type B only, but will procure other types on indent within three weeks.
For Normal Light Microscopy use Types A and B.
Elevated Temperatures (>23°C to 37°C): Use Type 37.
Inverted, Inclined, Projection, and Long Focus Instruments: Use type NVH or OVH. the greater the gap between the cover glass and objective, or between the slide and condenser, the more desirable high viscosity becomes. Their very high viscosities give excellent results for these applications.
Fluorescence Microscopy: Extremely low fluorescence is achieved by Type LDF and Type HF.
Types A & B Immersion Oils
These two types practically vary only in viscosity and they are interchangeable and miscible with each other for intermediate viscosities. Produced in larger quantities than other types, Types A and B are the most economical. The deciding factor in choosing between them is the optimum viscosity for your particular application.
Type A, at 150 centistokes, reduces any tendency to trap air, especially helpful to beginning students. Air bubbles cause image degradation. Also Type A is less sticky and easier to clean up.
Type B, at 1250 cSt, is thick enough for viewing multiple slides with one application. This saves time during batch processing. Type B is the most used of these immersion oils. For a greater gap between cover glass and objective, (or condenser and slide) type B is more desirable.
Oil Type A & Oil Type B
For horizontal, inverted and inclined instruments and projection equipment, Type NVH with high viscosity of 21,000 cST or Type OVH with a viscosity of 46,000 cST, (about 3 times that of honey) should be used. These types combine high or very high viscosity with "normal" refractive index properties. The greater the gap between the cover glass and objective, or between the slide and condenser, the more desirable high viscosity becomes. Their very high viscosities give excellent results for these applications.
Elevated temperatures can be due to substage illuminators, "hot stage", or other causes - ideal situations for Cargille Immersion Oil Type 37.
Immersion Oil Type 37 is useful for microscopic studies at body temperatures or when high room and stage temperatures may approach 37°C. Developed specifically for working at human body temperatures, Type 37 has a refractive index of 1.515 and a viscosity of 1250 cSt at 37°C solving the problem of image degradation above the standard calibration temperature of 23°C. Since the refractive index/ temperature relationship is inverse, increasing temperature significantly reduces the refractive index and lowers the viscosity of immersion oils. Cargille resolved this problem with Type 37 immersion oil. For any Elevated Temperatures (>23°C to 37°C) use Type 37. Users can blend for their own working temperature; blending Type B, with a viscosity of 1250 cSt at 23°C with Type 37, 1250 cSt at 37°C maintains a constant 1250 cSt viscosity and optical values and places the temperature of calibration proportionally between 23°C to 37°C.
IC116LDF This is in most regards our best immersion oil for fluorescence microscopy. It has extremely low background fluorescence (Cargille # 1-2). It has excellent optical values of refractive index (1.515 at sodium yellow 5893 angstroms and 1.518 at mercury green 5461 angstroms) and dispersion (41.3 for Abbe υD) for a sharp image. It has a medium viscosity of 500 cSt. It has an odour, (1-bromonaphthalene), that some people dislike.
IC117FF This oil is halogen free, has no odour, and is also nearly free of background fluorescence (Cargille # 1 max.). For optical properties its refractive index is 1.479 at 5893 angstroms which is lower than the ideal 1.515 so image sharpness is less, but probably not noticeably. It has a low viscosity of 170 cSt.
Automated Hematology Systems: Use Type 300; Automated Hematology Systems depend on accurate, precisely controlled physical and optical properties of immersion oil for successful imaging and mechanical processing. Type 300 is designed and manufactured to meet the stringent requirements of this equipment, which include specialised viscosity and exacting controls for its consistency.
|Immersion Oil Type||A||B||NVH||OVH||37||FF||LDF|
|Refractive Index :||@23°C||@23°C||@23°C||@23°C||@23°C||@23°C||@23°C|
|F Line (4861 A)||1.5239||1.5236||1.5230||1.5230||1.5238*||1.4850||1.5239|
|E Line (5461 A)||1.5180||1.5180||1.5178||1.5178||1.5180*||1.4811||1.5181|
|D Line (5893 A)||1.5150||1.5150||1.5150||1.5150||1.5150*||1.4790||1.5150|
|C Line (6563 A)||1.5114||1.5116||1.5118||1.5118||1.5115*||1.4766||1.5115|
|Temperature Coefficient (15-35°C) _ dnD/dt||0.00033||0.00031||0.00034||0.00034||0.00035*||0.00037||0.00036|
|Stability (change in nD @25°C after 24 hrs at stated temperature) *|
|Fluorescence*: Short UV:||low||low||low||low||low||none||very low|
|Fluorescence*: Long UV:||low||low||low||low||low||none||very low|
|Viscosity: cSt ± 10% @ 23°C (centistokes)||150
|Density: @ 23°C gm/cc:||.923||.923||.919||.918||.917||0.877||0.984|
|Density: (US) lb/gal||7.70||7.70||7.67||7.66||7.73||7.32||8.21|
|Neutralisation: (mg KOH/gm)||0.01 max||0.01 max||0.01 max||0.04 max||0.01 max||0.01 max||0.01 max|
a Relative to Cedarwood Oil
* These values are for type 37 are standardised at 37°C
b Type 37 is 3800 cSt at 23°C
|Code||Title||Type||Pack Size||Availability||Price||Updated: 07-12-2021|
|IC113||Immersion oil||Type A||50mL||In Stock||AU $28.00|
|IC1132||Immersion oil||Type A||200mL||Only 2||AU $115.00|
|IC1133||Immersion oil||Type A||3.785L||1 in stock||AU $1,312.00|
|IC114||Immersion oil||Type B||50mL||In Stock||AU $28.00|
|IC1142||Immersion oil||Type B||200mL||Only 2||AU $110.00|
|IC1145||Immersion oil||Type B||480mL||4 weeks||AU $230.00|
|IC1143||Immersion oil||Type B||3.785L||In Stock||AU $1,237.00|
|IC137||Immersion oil||Type 37||50mL||In Stock||AU $49.00|
|IC1372||Immersion oil||Type 37||200mL||Only 2||AU $172.00|
|IC1375||Immersion oil||Type 37||450mL||Only 1||AU $362.00|
|IC118||Immersion oil||Type OVH||50mL||In Stock||AU $51.00|
|IC1182||Immersion oil||Type OVH||200mL||Only 2||AU $177.00|
|IC1185||Immersion oil||Type OVH||450mL||1 in stock||AU $369.00|
|IC115||Immersion oil||Type NVH||50mL||In Stock||AU $46.00|
|IC1152||Immersion oil||Type NVH||200mL||Only 2||AU $163.00|
|IC1151||Immersion oil||Type NVH||450mL||Only 1||AU $280.00|
|IC16252||Immersion oil||Type 300||7.4mL||In Stock||AU $51.00|