Quantifoil R grids, holey carbon film, 2.0um/2.0um

QUANTIFOIL® is a perforated support foil with pre-defined hole size, shape, and pattern. When compared to ordinary holey films, it has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy.QUANTIFOIL® is available with circular and square holes that are orthogonally placed. The foil's standard substance is carbon. There are films with various hole sizes and bar widths available.  The superior quality of holey carbon film, which facilitates the use of automation in TEM. (The Imaging Technology Group of Dr. Bridget Carrageen, University of Illinois at Urbana-Champaign, has developed a system called Legion, for automatically acquiring images from a transmission electron microscope)QUANTIFOIL® with circular holes are used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The hole size chosen depends on the magnification used...

QUANTIFOIL® is a perforated support foil with pre-defined hole size, shape, and pattern. When compared to ordinary holey films, it has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy.

QUANTIFOIL® is available with circular and square holes that are orthogonally placed. The foil's standard substance is carbon. There are films with various hole sizes and bar widths available.  The superior quality of holey carbon film, which facilitates the use of automation in TEM. (The Imaging Technology Group of Dr. Bridget Carrageen, University of Illinois at Urbana-Champaign, has developed a system called Legion, for automatically acquiring images from a transmission electron microscope)

QUANTIFOIL® with circular holes are used in cryo-electron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The hole size chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.

The surface properties of QUANTIFOIL® holey carbon support film, especially the wetting properties, may have to be adapted according to one’s particular requirements. Untreated aging QUANTIFOIL® tends to be hydrophobic. The foil can be made hydrophilic by glow discharging in residual air or by metal coating.

QUANTIFOIL® with a regular pattern is required in order to be able to distinguish an object, which is spanned over a hole. An object cannot be discriminated from the support in the case of conventional holey support film. (H.W. Fink & C. Schonenberger, University of Basel, used QUANTIFOIL® for the measurement of electrical current through DNA molecules.)

The standard foil is ~12nm thick and mounted on copper or gold grids with either square or round holes of different sizes. 

QUANTIFOIL is generally delivered as a carbon foil; it can be reinforced with plastic film. Other thicknesses between 10 and 25nm can be made upon request. 

** Please choose carefully. Returns of these items are subject to approval.

If you require gold grids, please ask for a quote.

  • QUANTIFOIL® R 1.2/1.3. A foil with ~1.2µm circular holes and a spacing of ~2.5µm between the holes. This type is used at magnifications around 50.000x.
  • QUANTIFOIL® R 2/2. Holey films with 2µm circular holes are used at magnifications between 30.000x and 40.000x. R2/2 has 2µm holes and 4µm spaces.
  • QUANTIFOIL® R 2/4 may be preferred over R 2/2, when an increased tolerance with respect to the position of beam, and a larger beam diameter are desired, such as in the case of automated image acquisition. R2/4 has 2µm holes and 6µm spaces.
  • QUANTIFOIL® R 2/1 has more open area than R 2/2. It is used when focusing is carried out on the edge of a hole burnt in the ice in a neighbouring hole instead of on the foil adjacent to the hole. R2/1 has 2µm holes and 3µm spaces.
  • QUANTIFOIL® with square holes and relatively narrow bars can be used in EM to support a thin carbon film, which by itself is too fragile to span a grid square. Alternatively, this holey film can directly support an object that is larger than the holes.
  • QUANTIFOIL® S 7/2 constitutes an optimum between a maximum of open area on the one hand, and mechanical stability on the other hand. S7/2 has 7x7µm holes and 9µm spaces.
  • QUANTIFOIL® Multi A is a holey film, which consists of various pattern hole sizes, shapes and arrangements. In addition to round holes, the pattern includes oval-shaped ones, which appear round at high tilt angles (~70°). The diameters of the round holes are about 1, 1.4 and 2µm, and the bar widths range from 0.5 to 4µm. The oval holes in the foil have a dimension of 8 x 2µm and 4 x 1µm.

LONG LEAD TIME

Code Title Mesh size Grid material Pack size Availability Price Updated: 02-07-2022
EMSQ210CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Copper Pack/10 No ETA AU $196.00
SQR22-200CU Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Copper Pack/25 No ETA AU $381.00
EMSQ250-CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Copper Pack/50 No ETA AU $739.00
EMSQ2100CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Copper Pack/100 No ETA AU $1,259.00
EMSQ210NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Nickel Pack/10 No ETA AU $219.00
EMSQ225-NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Nickel Pack/25 No ETA AU $419.00
EMSQ250NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Nickel Pack/50 No ETA AU $747.00
EMSQ2100NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Nickel Pack/100 No ETA AU $1,359.00
EMSQ210AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Gold Pack/10 No ETA AU $222.00
EMSQ225AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Gold Pack/25 No ETA AU $489.00
EMSQ250-AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Gold Pack/50 No ETA AU $1,005.00
EMSQ2100AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 200 mesh Gold Pack/100 No ETA AU $1,696.00
EMSQ310CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Copper Pack/10 No ETA AU $196.00
EMSQ325CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Copper Pack/25 No ETA AU $381.00
EMSQ350CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Copper Pack/50 No ETA AU $739.00
EMSQ3100CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Copper Pack/100 No ETA AU $1,259.00
EMSQ310NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Nickel Pack/10 No ETA AU $219.00
EMSQ325NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Nickel Pack/25 No ETA AU $419.00
EMSQ350NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Nickel Pack/50 No ETA AU $747.00
EMSQ3100NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Nickel Pack/100 No ETA AU $1,359.00
EMSQ310AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Gold Pack/10 No ETA AU $222.00
EMSQ325AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Gold Pack/25 No ETA AU $489.00
EMSQ350AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Gold Pack/50 No ETA AU $1,022.00
EMSQ3100AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 300 mesh Gold Pack/100 No ETA AU $1,750.00
EMSQ410CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Copper Pack/10 No ETA AU $196.00
EMSQ425CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Copper Pack/25 No ETA AU $381.00
EMSQ450CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Copper Pack/50 No ETA AU $739.00
EMSQ4100CR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Copper Pack/100 No ETA AU $1,259.00
EMSQ410NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Nickel Pack/10 No ETA AU $219.00
EMSQ425NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Nickel Pack/25 No ETA AU $419.00
EMSQ450NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Nickel Pack/50 No ETA AU $747.00
EMSQ4100NR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Nickel Pack/100 No ETA AU $1,359.00
EMSQ410AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Gold Pack/10 No ETA AU $222.00
EMSQ425AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Gold Pack/25 No ETA AU $489.00
EMSQ450AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Gold Pack/50 No ETA AU $978.00
EMSQ4100AR2 Quantifoil R grids, holey carbon film, 2.0um/2.0um 400 mesh Gold Pack/100 No ETA AU $1,750.00