AURION BSA-c Reagents

In the blocking step, hydrophobic moieties causing “stickiness” in the specimen surface are rendered hydrophilic to minimise background. Nevertheless, the more dynamic charge-based interaction between the specimen surface and immunoreagents also needs to be controlled in order to eliminate background.

Aurion has developed BSA-c™ , a unique incubation buffer additive with an unparalleled ability to effectively prevent charge based background. BSA-c™ is prepared by acetylation of bovine serum albumin (BSA). Polycationic sites in the specimen interact readily with negatively charged acetylated BSA molecules. This significantly reduces the risk that such sites might bind negatively-charged immunoreagents and immunogold conjugates and thus reduces the risk of background.

Procedures to eliminate background comprise three main steps:

1. To suppress residual aldehyde activity
2. To saturate multipoint hydrophobic moieties and positive charges with high molecular weight compounds such as those present in the AURION Blocking Solutions
3. To reduce non-specific binding of immunoreagents caused by hydrophilic interaction with competing molecules in the incubation and washing solution.

Storage:

AURION BSA-c™ concentrate has a guaranteed shelf life of 18 months from the date of quality control analysis. The products should be stored at 4-8°C. Freezing is not recommended.