Conventional Immuno Gold Reagents
Conventional Immunogold Reagents are available in four size classes. The monodisperse size population makes the conjugates suited for multiple labeling with no overlap. The Conventional Immunogold Reagents are the classical conjugates in immuno electron microscopy; they are a good choice when the antigen is abundant and the accessibility of the antigen is relatively good.
Introduction
The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary immunogold reagents based on particles that can be observed without enhancement. These conjugates are suited for single and multiple labeling in electron microscopy, when the number of antigens available for binding is such that a relevant signal can be obtained.
The AURION Conventional Immunogold Reagents are built around colloidal gold particles with sizes of 6, 10, 15 or 25 nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6 and 25 nm particle size conjugates is less than 12%, whereas the 10 and 15 nm size conjugates show less then 10% variation.
The table below lists a few physical characteristics of gold conjugates:
| Particle diameter |
#Au atoms |
MWt. (daltons) |
#particles /ml |
#Ab /(part.) |
|---|---|---|---|---|
| 6nm | 6500 | 1.3·10^6 | 2.4·10^13 | 1-2 |
| 10nm | 30·10^3 | 6·10^6 | 5·10^12 | 7-12 |
| 15nm | 100·10^3 | 20·10^6 | 1.5·10^12 | 25-40 |
| 25nm | 470·10^3 | 92·10^6 | 3.3·10^11 | 115-180 |
Specificity:
Aurion offers the widest range of Conventional Immunogold Reagents. Please refer to the list of available reagents under ‘Ordering Information’.
AURION Conventional Immunogold Reagents are prepared using the highest quality antibodies or binding agents available. All antibodies are immuno affinity purified and immuno cross-adsorbed to reduce non-specific reactions.
Storage:
AURION Conventional Immunogold Reagents have a guaranteed shelf life of 18 months from the date of quality control analysis. The products should be stored at 4-8°C. Freezing is not recommended.
CHEM Conventional Immuno Gold Reagents
| Code | Title | Option 1 | Option 2 | Option 3 | Availability* | Price | |
|---|---|---|---|---|---|---|---|
| JA806-344 | Aurion donkey-anti-sheep IgG (H&L) | 6nm | 1ml | No ETA | AU $552.00 | ||
| JA106-344 | Aurion donkey-anti-sheep IgG (H&L) | 6nm | 2.5ml | No ETA | AU $843.00 | ||
| JA810-344 | Aurion donkey-anti-sheep IgG (H&L) | 10nm | 1ml | No ETA | AU $552.00 | ||
| JA110-344 | Aurion donkey-anti-sheep IgG (H&L) | 10nm | 2.5ml | No ETA | AU $843.00 | ||
| JA815-344 | Aurion donkey-anti-sheep IgG (H&L) | 15nm | 1.4ml | No ETA | AU $552.00 | ||
| JA115-344 | Aurion donkey-anti-sheep IgG (H&L) | 15nm | 3.5ml | No ETA | AU $843.00 | ||
| JA825-344 | Aurion donkey-anti-sheep IgG (H&L) | 25nm | 1.4ml | No ETA | AU $552.00 | ||
| JA125-344 | Aurion donkey-anti-sheep IgG (H&L) | 25nm | 3.5ml | No ETA | AU $843.00 | ||
| JA806-033 | Aurion goat-anti-mouse IgM (µ-chain) | 6nm | 1ml | No ETA | AU $518.00 | ||
| JA106-033 | Aurion goat-anti-mouse IgM (µ-chain) | 6nm | 2.5ml | No ETA | AU $814.00 | ||
| JA810-033 | Aurion goat-anti-mouse IgM (µ-chain) | 10nm | 1ml | No ETA | AU $518.00 | ||
| JA110-033 | Aurion goat-anti-mouse IgM (µ-chain) | 10nm | 2.5ml | No ETA | AU $809.00 | ||
| JA815-033 | Aurion goat-anti-mouse IgM (µ-chain) | 15nm | 1.4ml | No ETA | AU $518.00 | ||
| JA115-033 | Aurion goat-anti-mouse IgM (µ-chain) | 15nm | 3.5ml | No ETA | AU $814.00 | ||
| JA825-033 | Aurion goat-anti-mouse IgM (µ-chain) | 25nm | 1.4ml | No ETA | AU $518.00 | ||
| JA125-033 | Aurion goat-anti-mouse IgM (µ-chain) | 25nm | 3.5ml | No ETA | AU $814.00 |