Microscopy Stain Index
We supply a complete line of prepared, ready-to-use, high quality staining solutions for all standard staining procedures for light microscopy and electron microscopy.
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All solutions and concentrations are available in prepared and convenient, precise quantities.
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Premixed stains are ready-to-use, saving you time and effort
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Consistent uniform results can be obtained with technical information on use and results available in the table below
Certified Stains:
Stain Name | Certification Number | Application Details | Extra Information |
Acid Fuchsin, Certified | C.N. #DCR-11 | Tissue cytoplasm and nuclei | No |
Acridine Orange, Certified | Certified | Human bone marrow and lymph node cells | |
Alcian Blue 8GX, Certified | C.N. #DCAN-25 | Cell surface and cartilage | No |
Alizarin Red, Certified | C.N. #DCAR-9 | Nervous tissue (small invertebrates), bone (mammalian embryos) | No |
Aniline Blue, Certified | C.N. #DCK-13 | Connective tissue, collagen, reticulum, muscle, plasma, and nuclei | No |
Auramine O, Certified | C.N. #DCAU-4 | Infected tissue | No |
Azocarmine G, Certified | Certified | Pancreas alpha, beta, and all D-cells | No |
Azure A, Certified | Certified | Polychromatic blood stain | No |
Azure B, Certified | Certified | Blood protozoa | No |
Azure II, Certified | Certified | Marrow cells, nuclei, and bacteria | No |
Azure II Eosin, Certified | Certified | Blood protozoa and plant tissue | No |
Basic Fuchsin, Certified | C.N. #DCFB-39 | Pituitary bacteria | No |
Malachite Green 4, Certified | C.N. #DCMG-11 | Botanical bacteria | No |
Biebrich Scarlet, Certified | Certified | Collagen, reticulum, muscle, and plasma, erythrocytes & eosinophil granules and chromatin | No |
Bismarck Brown Y, Certified | Certified | Mucin & calciform cells of intestine, cartilage & embryo | No |
Brilliant Cresyl Blue, Certified | C.N. #DCV-5 | Platelets and reticulum of red cells | No |
Carmine, Certified | C.N. #DCCA-20 | Chromosomes, nuclei, and mucins | No |
Cresyl Fast Violet, Certified | C.N. #DCW-9 | Nerve cells | No |
Crystal Violet, Certified | C.N. #DCC-68 | Bacteria, filaments, glial fibres, amyloid in human tissue, chromatin and nucleoli in plant tissue | No |
Eosin Y, Certified | C.N. #DCE-58 | Cell nuclei in embryos and kidneys, blood corpuscles | |
Erythrosin B, Certified | C.N. #DCER-5 | Nerve cells and plant tissue | No |
Fast Green FCF Certified | C.N. #DCGF-16 | Mammalian collagen, muscle, cystoplasma, cells | No |
Fluorescein Isothiocyanate, Isomer I, Certified | Certified | Antibodies and specific antigens | No |
Giemsa Stain Powder, Certified | C.N. #DCGE-23 | Leucocytes, bacteria, inclusion bodies, bone marrow and connective tissue | No |
Giemsa Stain Solution, Certified | C.N. #DCGE-23 | Leucocytes, bacteria, inclusion bodies, bone marrow and connective tissue | |
Haematoxylin, Certified | C.N. #DCH-82 | Histology, cytology and plastic embedded tissues, nuclei & nuclear chromatin | No |
Indigo Carmine, Certified | C.N. #DCI-7 | Negri bodies, animal embryos, vaginal tissue, plant tissue | No |
Light Green SF Yellowish, Certified | C.N. #DCL-38 | Fungi, bone and calcium, glycogen and connective tissue | No |
Methyl Green, Certified | C.N. #DCG-20 | Myeloperoxidase, intestine calciformcells, trachea cartilage, embryonic tissue and bacteria | No |
Methylene Blue, Certified | C.N. #DCA-37 | Bacteria, acid-fast baccilli, rickettsia, mitochrondria and Negri bodies | No |
Methyl Violet 2B, Certified | C.N. #DCMV-10 | Amyloid, nuclei, metachromatic granules of diphtheria organisms | No |
Nigrosin, Certified | Certified | Nerve cells and bacteria | No |
Nile Blue A, Certified | C.N. #DCNB-10 | Fat, lipids, melamines, lipofuchsins and phospholipids | No |
Oil Red O, Certified | C.N. #ACRO-19 | Fat, lipids in fresh tissue | No |
Orange II, Certified | Certified | Tissues | No |
Orange G, Certified | C.N. #DCO-20 | Fibrin, keratin, collagen, and erythrocytes, alpha, beta, and gamma cells, nissl substances and PAS-positive material | No |
Phloxine B, Certified | C.N. #ECPH-1 | Inclusion bodies, nuclei, malarial parasites, haemaglobin and haemosiderin, keratin, prekeratin and mucin, beta cells | No |
Pyronin Y, Certified | C.N. #DCPY-2 | Gonorrheal pus, liver cells and protein | No |
Safranin O, Certified | C.N. #DCS-40 | Haemosiderin, nuclei and granules, chromatin and nuclear elements | No |
Sudan Black B, Certified | C.N. #DCZB-6 | Animal fat tissue, chromosomes, golgi, and leucocyte granules | No |
Toluidine Blue O, Certified | C.N. #DCU-10 | Bone and cartilage, mammalian embryos, methachromatic tissue, DNA RNA and malignant cells | No |
Wright's Stain, Certified | C.N. #DCWR-46 | Blood corpuscles, platelets and reticulum of red cells, blood and bone marrow films | No |
STAIN METHOD INDEX TABLE
Stain Name | Components | Application Details | Extra Information |
3-Amino-9-Ethylcarbazole Method for Leukocyte Eroxidase | Formalin-Acetone Fixative 3-Amino-9-Ethylcarbazole Staining Solution Mayer's Haematoxylin |
Leukocytes | |
AB/PAS/CG Stain | Alcian Blue 8Gx Solution 1%, pH 2.6-3.0 Sodium Carbonate 0.3% Aqueous Solution Periodic Acid 1% Aqueous Solution Schiff’s Reagent Phophomolybdic Acid-Orange G Solution |
Acidophils, basophils, and chromophobes | |
Alcian Blue Method (pH 2.5) | Alcian Blue 1% Nuclear Fast Red Solution Acetic Acid 3% Aqueous Solution |
Mucosubstances, hyaluronic acid and, sialomucins | |
Alcoholic Congo Red Solution | Congo Red 1% Aqueous Solution | Amyloids, nuceli and elastic tissue | |
Aldehyde-Fuchsin Method, pH 1.0 | Aldehyde-Fuchsin Solution pH 1.0 Metanil Yellow Solution 0.25% Alcohol Solution 80% pH 1.0 |
Mucosubstances | |
Alizarin Red for Calcium Deposits | Alizarin S 2% Solution pH 4.2 Reagent Alcohol 50% Acetone Acetone-Xylene |
Calcium deposits | |
Alizarin Red S and Toluidine Blue O | Alizarin Red S 0.02% Aqueous Solution Toluidine Blue O 0.25% Ammonium Alcohol Potassium Hydroxide Sulphuric Acid Alcohol Potassium Hydroxide 4% Aqueous Solution |
Bacteria | |
Bismuth Stain Kit 1 | Solution A (Sodium tartrate and Sodium hydroxide and Bismuth Subnitrate) Solution B (0.2M Triethanolamine-HCl buffer |
Specific En Bloc Staining | |
Bismuth Stain Kit 2 | Solution A (Sodium Tartrate in NaOH) Bismuth Subnitrate |
Enhancing Contrast in Uranyl Acetate Treated Tissue | |
Bismuth Stain Kit 3 | Solution A (Periodic acidEthanol and Sodium acetate in distilled water) Solution B (Sodium tartrateNaOH and Bismuth subnitrate) |
Mucosubstances and Polysaccharides | |
Bodian's Protargol Method | Protargol Solution 1% Aqueous Solution Cooper Shot Gold Chloride 1% Aqueous Solution Aniline Blue Solution Lissamine Fast Red Solution Oxalic Acid2% Aqueous Sodium Thiosulphate 5% Aqueous Solution Phosphomolybdic Acid 1% Aqueous Tartrazine Solution 1.5% Hydraquinone Reducing Solution |
Nerve fibre and ending | |
Bromophenol Blue | - | Proteins and nucleic acids | |
Brown and Brenn Method for Gram Positive and Gram Negative Bacteria | Crystal Violet Stain Solution 1% Aqueous Solution Sodium Biocarbonate 5% Aqueous Solution Basic Fuchsin Stock Solution 0.25% Basic Fuchsin Working Solution Gram's Iodine Solution Acetone-Alcohol 1:1 Picric Acid Acetone Solution 0.1% Acetone Acetone:Xylene |
Bacteria | |
Brown-Hopps Method for Gram Positive and Gram Negative Bacteria | Crystal Violet Stain 1% Aqueous Solution Gram's Iodine Solution Basic Fuchsin Staining Solution 0.5% Aqueous Solution Gallego’s Differentiating Solution Cellosolve Tartrazine 1.5% Aqueous Solution |
Bacteria | |
Canaliculi and Lacunae Stain for Hard Tissues | Cupric Nitrate 1% Aqueous Solution Protargol Solution 1% Aqueous Solution Copper Shot Hydroquinone Reducing Solution Gold Chloride 1% Aqueous Solution Oxalic Acid 2% Aqueous Solution Sodium Thiosulphate 5% Aqueous Solution |
Bones and teeth | |
Carstairs Method for Fibrin and Platelets | Ferric Ammonium Sulphate 5% Mayer's Haematoxylin Picric Acid-Orange G Solution Ponceau Acid Fuchsin Solution Phosphotungstic Acid 1% Aqueous Solution Aniline Blue Solution |
Fibrin and platelets | |
Cresyl Fast Violet, (FIRM) | - | Fluorescent dye infiltrates resin for tissue structures in negative relief | |
Crystal Violet Stain for Amyloid | Crystal Violet Solution Acetic Acid 1% Aqueous Solution |
Amyloids and tissue | |
Dane's Method for Prekeratin, Keratin, and Mucin | Phloxine Solution 1% Alcian Blue 0.5% Orange G Solution Mayer's Haematoxylin |
Keratin, prekeratin, acid mucopolysaccharides and nuclei | |
Differential Quik III Staining Kit (Modified Giemsa) | - | Rapid blood smears, Helicobactor Pylori and microorganisms | |
Ehrlich's Haematoxylin | Ehrlich's Haematoxylin Solution Acid Alcohol 1% Sodium Acetate 1% Aqueous Solution Ammonium Water 0.3% Lithium Carbonate, Saturated |
Nuclei | |
Fat Stains in Supersaturated Alcohol | Sudan IV-Isopropanol Solution Oil Red O-Isopropanol Solution Sudan III-Isopropanol Solution Mayer's Haematoxylin Erhlich’s Haematoxylin, Diluted Sodium Phosphate 1% Aqueous Solution |
Fat and lipids | |
Ferritin Cationised | - | Negative charges | |
Genta, Robason and Graham Stain for Helicobactor Pylori and Gastric Morphology | Uranium Nitrate Silver Nitrate 1% Aqueous Solution Gum Mastic 2.5% Hydroquinone 2% Silver Nitrate 0.04% Alcian BluepH 2.5 Harris Haematoxylin Acid Alcohol 1% Ammonia Water 0.25% Eosin Y 1% |
Helicobactor Pylori and gastric morphology | |
Giemsa for Helicobactor Pylori and Mast Cells | Methanol Giemsa Stock Solution Acetic Acid 1% |
Bacterial and fungal mast cells | |
Giemsa Thick Film Stain | Giemsa Thick Film Solution Giemsa Stock solution Phosphate Buffer |
Malarial parasites | |
Giemsa Thin Film Stain | Giemsa Thin Film Solution Giemsa Stock Solution Phosphate Buffer |
Eucocytes, rickettsia, bacteria and inclusion bodies | |
Gold Chloride Hydrate (Chloroauric Acid; Hydrogen Tetrachloroaurate) |
Sodium Citrate |
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Goldner's Trichrome Method | Bouin’s Fluid Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B Ponceau Acid Fuchsin Acetic Acid 1% Phosphomolybdic Acid Orange G Solution Light Green Stock 0.2% |
Elastic tissue | |
Gomori's Methenamine Silver for Urate Crystals Urate crystals | Silver Nitrate 5% Methenamine 30% Sodium Borate 5% Gold Chloride 0.1% Sodium Thiosulphate 3% Light Green Working Solution |
Urate Crystals | |
Gridley's Method for Endamoeba Histolytica | Harris Haematoxylin Weigert's Iron Haematoxylin Solution A Weigert's Iron Haematoxylin Solution B Analine Eosin Solution Naphthol Green B Stain 1% Acid Alcohol 1% In 75% Alcohol Ammonia Water 0.3% |
Bacteria amoebae, nuclei and ingested erythrocytes | |
Gridley's Method for Fungi | Chromic Acid 4% Coleman's Feulgen Aldehyde Fuchsin Metanil Yellow 0.25% |
Mycelia, conidia, yeast forms and hyphae | |
Haematoxylin And Eosin / Methenamine Silver Stain for Fungi In Tissue Sections | Chromic Acid 10% Sodium Bisulphite 1% Silver Nitrate 5% Aqueous Solution Methenamine 3% Aqueous Solution Sodium Borate 5% Aqueous Solution Gold Chloride 0.2% Sodium Thiosulphate 0.2% Aqueous Solution Harris’ Haematoxylin Acetic Acid 3% Ammonia Water 0.25% Eosin Y1% Alcoholic |
Fungi and tissue | |
Hall's Method for Bilirubin | Fouchets Reagent Van Gieson's Solution |
Bilirubin | |
Harris' Haematoxylin | - | Nuclei, fat, and lipids. | |
Harris' Haematoxylin (1900) | Harris' Haematoxylin Solution Harris' Haematoxylin Without Mercury Acid Alcohol 1% Sodium Acetate 1% Aqueous Solution Ammonia Water 0.3% Lithium Carbonate Saturated Solution |
Nuclei | |
Herxheimer's Technique | Sudan IV Staining Solution Harris' Haematoxylin Acetic Acid 1% Aqueous Solution |
Fat and cholesterol | |
Hirano-Zimmerman Method for Nerve Cells and Fibres (1962) | Silver Nitrate 10% Aqueous Solution Ammonia Water 0.1% Formalin Solution 50% Gold Chloride 0.05% Aqueous Solution Sodium Thiosulphate 5% Aqueous Solution |
Nerve cells, fibres, nerofibrils, dendrites, axis cylinders, senile plaques | |
Jenner's Stain Solution | - | Blood and bone marrow | |
Johnson's Method for Iron (Microincineration) | Potassium Ferrocyanide Solution 5% Hydrochloric Acid 5% |
Iron | |
Jones Method for Kidney | Periodic Acid 0.5% Aqueous Solution Methenamine Solution 3% Aqueous Silver Nitrate 5% Aqueous Solution Borate Buffer Working Solution Gold Chloride 0.2% Aqueous Solution Harris Haematoxylin Alcoholic Eosin Y Stain Solution Sodium Thiosulphate 3% Aqueous Solution Potassium Ferricyanide 0.5% Sodium Metabisulphite 3% Aqueous Solution Acid Alcohol 1% Ammonia Water 0.3% |
Basement membranes, reticulum fibres, collagen, and nuclei | |
Kinyoun's Method for Acid Fast Bacteria, Microwave | Kinyoun's Solution Acid Alcohol 1% Methylene Blue Working Solution |
Acid-fast bacteria | |
Kluver-Barrera Method for Myelin and Nerve Calls | Luxol Fast Blue MBS Solution 0.1% Cresyl Violet Acetate Solution 0.1% Acetic Acid 10% Aqueous Solution Lithium Carbonate Solution 0.05% |
Myelin and nerve cells | |
Laqueur's Method for Alcoholic Hyalin | Mayer's Haematoxylin Acid-Fuchsin Aniline Solution Light Green 1% Aqueous Solution Phosphomolybdic Acid 1% Alcoholic Picric Acid |
Mallory bodies, erythrocytes, bile pigment and proteinaceous, material in liver | |
Leukocyte Alkaline Phosphatase | Buffered Acetone Fixative Naphthol AS-BI Phosphate Dimethyl Formamide Fast Red Violet Salt LB Propanediol Buffer Haematoxylin Counter Stain |
Leukocytes | |
Lillie Modification of Masson's Trichrome | Phosphomolybdic/Phosphotungstic Acid Solution Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B Biebrich Scarlet Solution 1% Fast Green FCF Solution 2.5% Acetic Acid 1% Aqueous Solution Lugol's Iodine Sodium Thiosulphate 5% Aqueous Solution Gram's Iodine Solution |
Cells, cytoplasm muscle and collagen of mammalian tissue | |
Lillie's Method for Ferric and Ferrous Iron | Potassium Ferrocyanide Potassium Ferricyanide Hydrochloric Acid 0.5% Basic Fuchsin 0.5% Acetic Acid 1% Aqueous Solution |
Ferric and ferrous iron | |
Lillie's Method for Nucleic Acids (1965) | Schiff's Reagent Hydrochloric Acid 1N Sodium Bisulphite 0.05M Aqueous Solution Fast Green FCF Solution 0.01% |
Nuclear chromatin, and chromatin of plasmodia | |
Maldonado's Method for Pancreatic Islet Cells | Phloxine 1% Aqueous Solution Phosphotungstic Acid 3% Solution Azure Ii Solution 0.05% Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B |
Alpha, beta, D-cells, exocrine cells of islets of langerhans | |
Mallory-Heidenhain Azan-Gomori's Modification, Islet Cells | Azocarmine Stain Solution Aniline-Alcohol 1% Ferric Ammonium sulphate 5% Aniline Blue-Orange Solution Orange G 0.2% In Alcohol |
Alpha, beta and D-cells of islets of langerhans | |
Masson's Trichrome for Connective Tissue | Bouin's Fixative Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B Biebrich Scarlet Solution 1% Phosphomolybdic/Phosphotungstic Acid Solution Aniline Blue Solution Acetic Acid 1% Aqueous Solution |
Connective tissue | |
Maximow's Method for Bone Marrow | Mayer's Haematoxylin Eosin Y 0.1% Aqueous Solution Azure II 0.1% Aqueous Solution Phosphate Buffer 0.1M, pH 6.8-7.0 |
Marrow cells | |
May-Grunwald Giemsa for Helicobactor Pylori | Jenner’s Stain Stock Solution Giemsa Stock Solution Acetic Acid 0.1% Solution |
Bacteria and fungi | |
May-Grunwald/Geimsa Combined Stain for Bone Marrow | Lugol’s or Gram’s Iodine solution Sodium Thiosulphate 5% Methyl Alcohol May-Grunwald Solution or Jenner's Solution Giemsa Stock Solution Acetic acid 1% |
Marrow cells | |
Mayer Mucicarmine Method | Mucicarmine Stock Solution Metanil Yellow 0.25% Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B |
Mucosubstances | |
McManus' Method (PAS) for Glycogen | Coleman's Feulgen Light Green Stock Solution 0.2% Periodic Acid 0.5% Aqueous Solution Acid-Alcohol 1% Ammonia Water 0.3% Schiff's Reagent Light Green Working Solution Harris Haematoxylin |
Glycogen | |
Methenamine Silver Method for Argentaffin Cells | Weigert's Iodine Solution Sodium Thiosulphate 5% Aqueous Solution Silver Nitrate 5% Aqueous Solution Methenamine 3% Aqueous Solution Borate Buffer pH 7.8 Gold Chloride 0.1% Aqueous Solution Safranin O Solution 0.1% |
Argentaffin granules of enterochromaffin cells | |
Microwave PAS-Alcian Blue Method For Mucosubstance | Alcian Blue Solution Periodic Acid 1% Aqueous Solution Schiff's Reagent Gill's Haematoxylin Ammonia Water 1% |
Mucosubstances | |
Modification of Mayer's Mucihematein | - | Epithelial cells of glandular tissue | |
Modified Alizarin Red S for Foetal Specimens | Alizirin S 0.01% Aqueous Solution Potassium Hydroxide 1% Aqueous Solution Potassium Hydroxide 10% Aqueous Solution Glycerol Potassium Hydroxide |
Minute bones and foetal ossification in mammalian embryos | |
Modified Movat's Stain | Alcian Blue 1% Alkaline Alcohol Orcein 0.2% Haematoxylin Alcoholic 5% Ferric Chloride 10% Lugol’s Iodine Woodstain Scarlet-Acid Fuchsin Working Solution Acetic Acid 0.5% Phosphotungstic Acid 5% Alcoholic Saffron |
Connective and elastic tissue | |
Modified Steiner Using Chapman's Modification | Zinc Formalin Silver Nitrate1% Aqueous Solution Gum Mastic 2.5% Hydroquinone 2% Silver Nitrate 0.04% Aqueous Solution |
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Modified Verhoeff Elastic-Van Gieson Stain | Alcoholic Haematoxylin Ferric Chloride 2% Aqueous Solution Lugol’s Iodine Ferric Chloride 0.4% Aqueous Solution Van Geison's Solution |
Connective tissue | |
Neat Stain Gram Stain Kit | - | Bacteria | |
Neat Stain Haematology Stain Kit | - | Morphological cells and blood smears | |
New Methylene Blue Solution | - | Reticulum of immature erythrocytes | |
Nile Blue A Stain | Nile Blue A Staining Solution Sulphuric Acid 1% Aqueous Solution |
Fat and lipid | |
Oil Red in Propylene Glycol Method | Oil Red O Solution Mayer's Haematoxylin Harris' Haematoxylin Propylene Glycol 85% Aqueous Solution Hydrochloric Acid 5% Ammonia Water 0.3% |
Lipids, cholesteryl esters, and lipoproteins | |
Oil Red O Method for Lipofuscin | Oil Red O Solution 0.5% Propylene Glycol 85% Aqueous Solution Mayer’s Hematoxylin |
Lipofuscin | |
Papanicolaou Stain for Membrane Filters | Gill’S Hematoxylin No 1 Hydrochloric Acid 0.05% Scott’s Water Solution Modified Orange G Solution Modified EA Staining Solution |
Membrane Filters | |
PAS-Alcian Blue Method For Mucosubstances | Alcian Blue Solution Periodic Acid 1% Schiff's Reagent Sodium Metabisulphite 0.5% Aqueous Solution |
Mucosubstances | |
Pearse's Method for Phospholipids | Luxol Fast Blue MBS Solution 0.1% Lithium Carbonate 0.05% Aqueous Solution Neutral Red Solution 1% Aqueous Solution |
Phospholipids and early lipofuchsin | |
Periodic Acid Leucofuchsin Method (PAS) | Schiff’s Reagent Periodic Acid 1% Aqueous Solution Sodium Bisulphite 0.05M Aqueous Solution Mayer’s Acid Hemalum Solution Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B Picric Acid Saturated Aqueous Solution Orange G Solution 1% |
General tissue | |
Picrosirius Red Staining of Cardiac Muscle | Phosphomolybdic Acid 0.2% Aqueous Solution Sirius Red 0.1% In Saturated Picric Acid Hydrochloric Acid 0.01N |
Cardiac muscle and connective tissue | |
Pinkerton's Method for Rickettsia | Methylene Blue Solution 1% Basic Fuchsin 0.25% Aqueous Solution Citric Acid 0.5% Aqueous Solution Gram's Iodine Solution Lugol's Iodine Sodium Thiosulphate 5% Aqueous Solution |
Rickettsia | |
Pizzolato's Method for Calcium Oxalate | Silver Nitrate 5% Aqueous Solution Nuclear Fast Red (Kennechtrot) Solution |
Calcium oxalate | |
Platinum Blue, EM Stain | - | Thin sections, en-block staining, and negative staining (alternate to uranyl acetate) | |
Poly-L-Lysine Solution | - | Binds to DNA, red cell membranes and any negatively, charged proteins | |
Price’s Giemsa Stain | Giemsa Stock Solution Phosphate Buffer pH 7.0 Acetic Acid 0.2% Aqueous Solution Alcoholic Iodine 2% |
Haematologic elements | |
Prussian Blue Method for Haemosiderin | Potassium Ferrocyanide 2% Aqueous Solution Hydrochloric Acid 2% Safranin O 0.2% Acetic Acid 1% Aqueous Solution |
Haemosiderin | |
Prussian Blue Stain for Non-Haemoglobin Iron | Potassium Ferrocyanide 2% Aqueous Solution Hydrochloric Acid 1% Wright's Giemsa Stain Phosphate Buffer pH 6.5 |
Particulate iron and non-granular cytoplasm | |
PTAH Method for Central Nervous Tissue | Potassium Permanganate 1% Phosphotungstic Acid-Haematoxylin Oxalic Acid 5% Aqueous Solution Lugol's Iodine Solution Russels Modified Zenkers Fluid |
Microglia, fibroglia, fibrin and coarse fibrils | |
Puchtler-Sweat Method for Basement Membranes | Resorcin-Fuchsin Solution Nuclear Fast Red Solution Periodic Acid 0.5% Aqueous Solution Sodium Bisulphite Solution |
Basement membranes | |
Relative Acidophilia Stain | Phloxine B Staining Solution Tartrazine Saturated Solution In Cellosolve N-Butyl Alcohol |
Acidophilia | |
Rhodanine Method for Cu | Rhodamine Sturated Solution Mayer’s Haematoxylin Sodium Borate 5% Aqueous Solution |
Copper | |
Rhodinile Blue Stain | - | Haematologic elements | |
Sweat-Puchtler Method for Amyloids | Sirius Red F3Ba Solution 1% Mayer's Haematoxylin Borate Buffer 0.1M, pH 9.0 Alkaline Alcohol |
Amyloids, nuceli and elastic tissue | |
Taft's Method for Nucleic Acid (1951) | Methyl Green Pyronin Solution Differentiating Solution |
DNA and RNA | |
Taylor's Method for Bacteria | Harris Haematoxylin Hucker’s Crystal Violet Basic Fuchsin Stock Solution 0.1% Gram’s Iodine Solution Lithium Carbonate Solution Acid Alcohol 1% Acetone-Alcohol 1:1 Acetone Picric Acid-Acetone Solution 0.1% Acetone-Xylene I Acetone-Xylene II |
Bacteria | |
Thomas' Method for Malarial Parasites | Phloxine B Solution 0.5% Methylene Blue Azure B Solution Acetic Acid 0.2% Aqueous Solution |
Malarial parasites | |
Tissue marking dyes | - | Frozen or fixed tissues | |
Toren's Method for Mast Cells | Giemsa Stain Solution Acetic Acid 0.5% Aqueous Solution Pollacks’s Trichrome Solution |
Mast cells | |
Truant's Fluorescent Method for Acid Fast Organism | Weigert’s Iron Haematoxylin Solution A Weigert’s Iron Haematoxylin Solution B Auramine-Rhodamine Staining Solution Acid Alcohol 1% |
Bacteria | |
UAR-EMS Uranyl Acetate Replacement Stain | - | Negative and positive stain, alternative to uranyl acetate | |
Uranyl Acetate, Reagent, A.C.S. | - | Thin sections, en-block staining, and negative staining | |
UranyLess EM Stain | - | TEM negative staining applications (alternate to uranyl acetate) | |
Van Gieson's Method for Collagen Fibres | Van Gieson's Solution Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B |
Collagen fibres | |
Verhoeff's Elastic Tissue Stain | Iron Solution Potassium Iodide 3% Aqueous Solution Alcoholic Haematoxylin 10% Van Gieson's Solution |
Connective tissue | |
Verhoff's Van Geison Stain | Alcoholic Haematoxylin 5% Ferric Chloride 10% Weigert's Iodine Ferric Chloride 2% Sodium Thiosulphate 5% Aqueous Solution Van Gieson's Solution |
Elastic fibres, nuclei, collagen & other tissue elements | |
Vogt's Method for Nerve Cell Products | Cresyl Violet Acetate 2% Aqueous Solution Acetate Buffer Cresyl Violet Acetate Working Solution |
Nerve cells | |
Weigert's Iron Haematoxylin with Methachromic Dyes | Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B Fast Green FCF Solution 0.02% Bismark Brown Y Solution 0.1% Safranin O 0.1% Acetic Acid 1% Aqueous Solution |
Nuclei | |
Weigert's Resorcin Fuchsin | Resorcin-Fuchsin Solution Van Gieson's Solution Weigert's Iron Haematoxylin A Weigert's Iron Haematoxylin B Acid Alcohol 1% |
Elastic fibres in blood vessel walls, nuclei & collagen | |
Wilder Modification of Bielschowsky's Method | Silver Nitrate 10.2% Aqueous Solution Ammonium Hydroxideconcentrated Sodium Hydroxide 3.1% Formalin 40% Harris' Haematoxylin Nuclear Fast Red Solution Phosphomolybdic Acid 10% Uranium Nitrate 1% Gold Chloride Sodium Thiosulphate 5% Aqueous Solution Van Gieson's Solution |
Collagen and reticulum | |
Woelcker's Method for Myelin Sheath | Ferric Ammonium Sulphate 2.5% Aqueous Solution Alcoholic Haematoxylin Stock 10% Lithium Carbonate Saturated Solution |
Myelin sheath, glial cells and nucleoli of neurons | |
Wolbach's Giemsa Method | Giemsa Stock Solution Giemsa Working Solution Rosin Alcoholic Stock Solution Rosin Alcoholic Working Solution Lugol's Iodine Gram's Iodine Solution Sodium Thiosulphate 5% Aqueous Solution |
Nuclei, collagen, riskettsia and bacteria | |
Wright Staining Solution | Wright Stain Solution Phosphate buffer pH 6.5 |
Blood | |
Wright-Giemsa Stain | Wright Giemsa Stain Phosphate Buffer pH 6.5 |
Blood corpuscles |