Eyepiece Reticles

An eyepiece reticle is a glass disc with a pattern on it that fits at the optical plane inside a microscope eyepiece. It is used to provide alignment, measurement, size or shape comparison, or area counting of specimens by having the reticle pattern superimposed over the specimen image. 

The term reticle, graticule and reticule are all used to describe these items.

Graticules Optics produce Pyser eyepiece reticles which are made using 1.5mm thick optical glass. The image is created using a vacuum evaporated chrome process and is correct reading through the glass. They are available in a variety of standard diameters to suit most microscopes in the marketplace. Custom sizes are available to special order.

 Pyser produce a wide range of reticle patterns which includes:

  • Lines and cross-lines for alignment
  • Scales and gauges for measurement
  • Grids for counting and referencing
  • Particle sizing to determine shape, size an quantity of materials of vapours
  • Protractors for measuring angles
  • Stereology for extracting quantitative information from 3D images
  • Many specialist pattern designed for scientists for specific applications
     

    The two things you should consider when choosing your eyepiece reticle:

    1. What pattern is suitable for your application?

    2. What is the diameter of your microscope eyepiece?

     

    Pyser Eyepiece Reticle Patterns  

      LINES AND CROSSES

    Single lines: NE50

    For measurement of large objects in conjunction with graduated mechanic stage and the alignment.

    Crosslines: NE8, NE81, NE82

    Used as for NE50 but for measurements in two directions.

    Broken Crosslines: NE56

    Used as cross lines. Broken lines enable fine detail to be seen at the breaks. A thin boundary would be lost behind a continuous line.

    Crossed Gauge Lines: NE53, NE54

    Used as crossed lines but for measuring distances between lines. Greater accuracy cam be obtained by locating the specimen detail between the reticle gauge air.

    EYEPIECE SCALES

    Horizontal and Vertical Scales: NE1, NE2, NE5, NE20, NE28, NE31, NE41, NE120

    Used for measuring lengths of specimen or distances between points on a variety of different shaped objects.

    Crossed Scales: NE17, NE18, NE70, NE72

    Used as horizontal and vertical scales and especially useful when interested in measurements in different axis.

    Scales and Crosslines: NE7, NE7N, NE77, NE777

    The inclusion of a crossline assists in ensuring alignment of the reticle with edges etc. in the specimen.

    SQUARES & GRIDS* 

    Squared Grids: NE10, NE11, NE34

    Simple grids are convenient for making sketches of the observed specimen. They are also useful for particle counting.

    Index Grids: NE10A, NE11A, NE34A, NE71, NE35

    Useful for particle counting particularly where reference is needed between workers. Also useful for area of specimen determinations.

    Chessboard Squares: NE15

    The dark squares are translucent. Used as an alternative to simple grids for area of specimen determination and particle counting. Alternate light and dark squares help to reduce eyestrain. Semi coating gives approximately 50% light transmission.

    Squares & Grids: NE38

    Combines three areas in one for convenience, giving area ratios A:B or 1:3 and B:C of 1:2.

    Miller Squares: NE57, NE57C

    The ratio of large to small square is 9:1 or 10:1. Originally designed for haematology, they can be utilised for rapid counting of any evenly spread field of particles.

    Whipple Grid: NE29, NE29B

    Originally designed for water particle analysis, but may be used for other aspects of particle counting. Grid shown: Ratio of full square to smallest is 50:1. Area is 2500:1 (Reference: Microscopy of water)

    CIRCLE GUAGES & PROTRACTORS

    Concentric Circles: NE42, NE43, NE44, NE47, NE48, NE22

    Can be used for two-way measurement when calibrated as a micrometer.

    Gauge Pairs: NE19

    Gauge pairs occupying a field of view of 10mm. Each gauge is proportional to its adjacent number. Approximate size of smallest pair = 0.1mm.

    Half Protractors: NE25

    These are used for measuring angles when placed in the eyepiece, much like ordinary protractors.

    Full Protractors: NE45

    These are used for measuring angles when placed in the eyepiece, much like ordinary protractors.

    PARTICLE SIZING & DISTRIBUTION

    Patterson Globes & Circles: NG1

    Porton: NG2

    New Porton: NG12

    British Standard Reticle: NG10

    Fairs: NG5

    Walton & Beckett Asbestos Fibre Analysis Reticle: G22

    Walton & Beckett Asbestos Fibre Analysis Reticle: G24

    Walton & Beckett Asbestos Fibre Analysis Reticle: G25

    STEREOLOGY

    Mertz Reticle, 36 pts: NGM1

    Weibel 1: NGW1

    Weibel 2: NGW2

    Weibel 3: GW3

    METALLURGY

    New Grain Sizing Patterns (EN10247/ISO4976): NG60, NG61

    ASTM Austenite 1:1 Grain Sizing Disc: G41

    ASTM E112 Plate 1 Grain Sizing Disc: G42

    ASTM Carbide Grain Sizing Chart: G43

    ASTM E45: G44

    ASTM E19-46 Grain Sizing Disc: G45

    ASTM E19-46 Grain Sizing Disc Root 2: G46

    Circular Grid ASTM, 24pts: G54

    Square Grid ASTM, 25pts: G55

     SPECIALIST DESIGNS

    Spray Droplet Sizing Reticle (Matthews): NG30

    Thompson: G23

    Chalkley Point Array: NG52

    Pharmaceutical PSA Pattern: NG57

    Counting Pattern: NG14

    Lennox Grain Analysis: NG21

    Kotter: G48

    Zeiss Integrating Eyepiece 1 (Henning Reseau Pattern 25pts): G49

    Zeiss Integrating Eyepiece 100: G47

    Integrating Eyepiece: G50

    * Note these may need to be calibrated according to the intended use.

    See here for further information on Pyser Certification Standards

     

     

    LM Pyser Reticles

    Code Title Option 1 Option 2 Option 3 Availability* Price
    GRA01A245062 G22 eyepiece reticles, Walton & Beckett 3:1 G22 24.5mm Each leadtime:2 months AU $388.00
    S01A24062 G22 eyepiece reticles, Walton & Beckett 3:1 G22 24mm Each leadtime:2 months AU $341.00
    GRA01A20.4062 G22 eyepiece reticles, Walton & Beckett 3:1 G22 20.4mm Each leadtime:2 months AU $0.00
    GRA01A23062 G22 eyepiece reticles, Walton & Beckett 3:1 G22 23mm Each leadtime:2 months AU $0.00
    GRA01A25062 G22 eyepiece reticles, Walton & Beckett 3:1 G22 25mm Each leadtime:2 months AU $0.00
    S8028 G22 eyepiece reticles, Walton & Beckett 3:1 G22 16mm Each Only 3 AU $388.00
    S8029 G22 eyepiece reticles, Walton & Beckett 3:1 G22 19mm Each leadtime:2 months AU $388.00
    S8030 G22 eyepiece reticles, Walton & Beckett 3:1 G22 21mm Each Only 1 AU $388.00
    S01A26062 G22 eyepiece reticles, Walton & Beckett 3:1 G22 26mm Each leadtime:2 months AU $388.00
    S01A27062 G22 eyepiece reticles, Walton & Beckett 3:1 G22 27mm Each leadtime:2 months AU $388.00
    GRA01B21302 NE71 eyepiece reticles, indexed grid NE71 21mm Each leadtime:2 months AU $0.00
    GRA01B23302 NE71 eyepiece reticles, indexed grid NE71 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5302 NE71 eyepiece reticles, indexed grid NE71 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24302 NE71 eyepiece reticles, indexed grid NE71 24mm Each leadtime:2 months AU $0.00
    GRA01B25302 NE71 eyepiece reticles, indexed grid NE71 25mm Each leadtime:2 months AU $0.00
    GRA01B26302 NE71 eyepiece reticles, indexed grid NE71 26mm Each leadtime:2 months AU $0.00
    GRA01B27302 NE71 eyepiece reticles, indexed grid NE71 27mm Each leadtime:2 months AU $0.00
    GRA01B16242 NE48 eyepiece reticles, concentric circles + crossline NE48 16mm Each leadtime:2 months AU $0.00
    GRA01B19242 NE48 eyepiece reticles, concentric circles + crossline NE48 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4242 NE48 eyepiece reticles, concentric circles + crossline NE48 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21242 NE48 eyepiece reticles, concentric circles + crossline NE48 21mm Each leadtime:2 months AU $0.00
    GRA01B23242 NE48 eyepiece reticles, concentric circles + crossline NE48 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5242 NE48 eyepiece reticles, concentric circles + crossline NE48 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24242 NE48 eyepiece reticles, concentric circles + crossline NE48 24mm Each leadtime:2 months AU $0.00
    GRA01B25242 NE48 eyepiece reticles, concentric circles + crossline NE48 25mm Each leadtime:2 months AU $0.00
    GRA01B26242 NE48 eyepiece reticles, concentric circles + crossline NE48 26mm Each leadtime:2 months AU $0.00
    GRA01B27242 NE48 eyepiece reticles, concentric circles + crossline NE48 27mm Each leadtime:2 months AU $0.00
    GRA01B21228 NE47 eyepiece reticles, concentric circles NE47 21mm Each leadtime:2 months AU $0.00
    GRA01B23228 NE47 eyepiece reticles, concentric circles NE47 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5228 NE47 eyepiece reticles, concentric circles NE47 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24228 NE47 eyepiece reticles, concentric circles NE47 24mm Each leadtime:2 months AU $0.00
    GRA01B25228 NE47 eyepiece reticles, concentric circles NE47 25mm Each leadtime:2 months AU $0.00
    GRA01B26228 NE47 eyepiece reticles, concentric circles NE47 26mm Each leadtime:2 months AU $0.00
    GRA01B27228 NE47 eyepiece reticles, concentric circles NE47 27mm Each leadtime:2 months AU $0.00
    GRA01B16226 NE44 eyepiece reticles, concentric circles NE44 16mm Each leadtime:2 months AU $0.00
    GRA01B19226 NE44 eyepiece reticles, concentric circles NE44 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4226 NE44 eyepiece reticles, concentric circles NE44 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21226 NE44 eyepiece reticles, concentric circles NE44 21mm Each leadtime:2 months AU $0.00
    GRA01B23226 NE44 eyepiece reticles, concentric circles NE44 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5226 NE44 eyepiece reticles, concentric circles NE44 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24226 NE44 eyepiece reticles, concentric circles NE44 24mm Each leadtime:2 months AU $0.00
    GRA01B25226 NE44 eyepiece reticles, concentric circles NE44 25mm Each leadtime:2 months AU $0.00
    GRA01B26226 NE44 eyepiece reticles, concentric circles NE44 26mm Each leadtime:2 months AU $0.00
    GRA01B27226 NE44 eyepiece reticles, concentric circles NE44 27mm Each leadtime:2 months AU $0.00
    GRA01B16225 NE43 eyepiece reticles, concentric circles NE43 16mm Each leadtime:2 months AU $0.00
    GRA01B19225 NE43 eyepiece reticles, concentric circles NE43 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4225 NE43 eyepiece reticles, concentric circles NE43 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21225 NE43 eyepiece reticles, concentric circles NE43 21mm Each leadtime:2 months AU $0.00
    GRA01B23225 NE43 eyepiece reticles, concentric circles NE43 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5225 NE43 eyepiece reticles, concentric circles NE43 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24225 NE43 eyepiece reticles, concentric circles NE43 24mm Each leadtime:2 months AU $0.00
    GRA01B25225 NE43 eyepiece reticles, concentric circles NE43 25mm Each leadtime:2 months AU $0.00
    GRA01B26225 NE43 eyepiece reticles, concentric circles NE43 26mm Each leadtime:2 months AU $0.00
    GRA01B27225 NE43 eyepiece reticles, concentric circles NE43 27mm Each leadtime:2 months AU $0.00
    GRA01B16222 NE38 eyepiece reticles, ratio squares NE38 16mm Each leadtime:2 months AU $0.00
    GRA01B19222 NE38 eyepiece reticles, ratio squares NE38 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4222 NE38 eyepiece reticles, ratio squares NE38 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21222 NE38 eyepiece reticles, ratio squares NE38 21mm Each leadtime:2 months AU $0.00
    GRA01B23222 NE38 eyepiece reticles, ratio squares NE38 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5222 NE38 eyepiece reticles, ratio squares NE38 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24222 NE38 eyepiece reticles, ratio squares NE38 24mm Each leadtime:2 months AU $0.00
    GRA01B25222 NE38 eyepiece reticles, ratio squares NE38 25mm Each leadtime:2 months AU $0.00
    GRA01B26222 NE38 eyepiece reticles, ratio squares NE38 26mm Each leadtime:2 months AU $0.00
    GRA01B27222 NE38 eyepiece reticles, ratio squares NE38 27mm Each leadtime:2 months AU $0.00
    GRA01B16221 NE35 eyepiece reticles, numbered grid NE35 16mm Each leadtime:2 months AU $0.00
    GRA01B19221 NE35 eyepiece reticles, numbered grid NE35 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4221 NE35 eyepiece reticles, numbered grid NE35 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21221 NE35 eyepiece reticles, numbered grid NE35 21mm Each leadtime:2 months AU $0.00
    GRA01B23221 NE35 eyepiece reticles, numbered grid NE35 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5221 NE35 eyepiece reticles, numbered grid NE35 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24221 NE35 eyepiece reticles, numbered grid NE35 24mm Each leadtime:2 months AU $0.00
    GRA01B25221 NE35 eyepiece reticles, numbered grid NE35 25mm Each leadtime:2 months AU $0.00
    GRA01B26221 NE35 eyepiece reticles, numbered grid NE35 26mm Each leadtime:2 months AU $0.00
    GRA01B27221 NE35 eyepiece reticles, numbered grid NE35 27mm Each leadtime:2 months AU $0.00
    GRA01B16220 NE34A eyepiece reticles, indexed grid NE34A 16mm Each leadtime:2 months AU $0.00
    GRA01B19220 NE34A eyepiece reticles, indexed grid NE34A 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4220 NE34A eyepiece reticles, indexed grid NE34A 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21220 NE34A eyepiece reticles, indexed grid NE34A 21mm Each leadtime:2 months AU $0.00
    GRA01B23220 NE34A eyepiece reticles, indexed grid NE34A 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5220 NE34A eyepiece reticles, indexed grid NE34A 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24220 NE34A eyepiece reticles, indexed grid NE34A 24mm Each leadtime:2 months AU $0.00
    GRA01B25220 NE34A eyepiece reticles, indexed grid NE34A 25mm Each leadtime:2 months AU $0.00
    GRA01B26220 NE34A eyepiece reticles, indexed grid NE34A 26mm Each leadtime:2 months AU $0.00
    GRA01B27220 NE34A eyepiece reticles, indexed grid NE34A 27mm Each leadtime:2 months AU $0.00
    GRA01B16300 NE34 eyepiece reticles, pitch squares NE34 16mm Each leadtime:2 months AU $0.00
    GRA01B19300 NE34 eyepiece reticles, pitch squares NE34 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4300 NE34 eyepiece reticles, pitch squares NE34 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21300 NE34 eyepiece reticles, pitch squares NE34 21mm Each leadtime:2 months AU $0.00
    GRA01B23300 NE34 eyepiece reticles, pitch squares NE34 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5300 NE34 eyepiece reticles, pitch squares NE34 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24300 NE34 eyepiece reticles, pitch squares NE34 24mm Each leadtime:2 months AU $0.00
    GRA01B25300 NE34 eyepiece reticles, pitch squares NE34 25mm Each leadtime:2 months AU $0.00
    GRA01B26300 NE34 eyepiece reticles, pitch squares NE34 26mm Each leadtime:2 months AU $0.00
    GRA01B27300 NE34 eyepiece reticles, pitch squares NE34 27mm Each leadtime:2 months AU $0.00
    GRA01B16219 NE31 eyepiece reticles, horizontal scale NE31 16mm Each leadtime:2 months AU $0.00
    GRA01B19219 NE31 eyepiece reticles, horizontal scale NE31 19mm Each leadtime:2 months AU $0.00
    GRA01B20.4219 NE31 eyepiece reticles, horizontal scale NE31 20.4mm Each leadtime:2 months AU $0.00
    GRA01B21219 NE31 eyepiece reticles, horizontal scale NE31 21mm Each leadtime:2 months AU $0.00
    GRA01B23219 NE31 eyepiece reticles, horizontal scale NE31 23mm Each leadtime:2 months AU $0.00
    GRA01B24.5219 NE31 eyepiece reticles, horizontal scale NE31 24.5mm Each leadtime:2 months AU $0.00
    GRA01B24219 NE31 eyepiece reticles, horizontal scale NE31 24mm Each leadtime:2 months AU $0.00
    GRA01B25219 NE31 eyepiece reticles, horizontal scale NE31 25mm Each leadtime:2 months AU $0.00
    GRA01B26219 NE31 eyepiece reticles, horizontal scale NE31 26mm Each leadtime:2 months AU $0.00
    GRA01B27219 NE31 eyepiece reticles, horizontal scale NE31 27mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-19 NE29B eyepiece reticles, whipple grid NE29B 19mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-20.4 NE29B eyepiece reticles, whipple grid NE29B 20.4mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-21 NE29B eyepiece reticles, whipple grid NE29B 21mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-23 NE29B eyepiece reticles, whipple grid NE29B 23mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-24 NE29B eyepiece reticles, whipple grid NE29B 24mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-24.5 NE29B eyepiece reticles, whipple grid NE29B 24.5mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-25 NE29B eyepiece reticles, whipple grid NE29B 25mm Each leadtime:2 months AU $0.00
    GRAPC-0464-KW-26 NE29B eyepiece reticles, whipple grid NE29B 26mm Each leadtime:2 months AU $0.00