The DiATOME DiFFERENCE, the highest quality, the most precise sectioning, incomparable durability. 

Diatome knives are compatible with all ultramicrotomes.

Diamond blades and knives are essential microtomy tools for sectioning, sample preparation, and other application processes in microscopy. Each diamond knife is designed with unique properties that enable precision sectioning with minimal artefacts in application-specific environments. 

Explore DiATOME's extensive line of specially designed knives:

Ultramicrotomy: Cutting ultra thin sections (typically less than 100 nanometers thick) from a sample block. A diverse selection of diamond blades and other microtomy tools engineered to facilitate consistent, precise specimen preparations.

  • Highest quality diamonds and optimal crystal orientation guarantee perfect ultra thin sections and a durable cutting edge
  • Section pick-up is facilitated as the boat is horizontal allowing the water to completely fill the boat all the way round
  • A hydrophilic surface makes it easy to wet the cutting edge, even with low water level

Ultra AFM: The first diamond knives for AFM at room and low temperatures. The exceptional sharpness and durability of these diamond knives ensure that sections are of high quality and facilitate the preservation of surface properties during the sample preparation.

  • Highest quality diamonds and optimal crystal orientation guarantee perfect ultra thin sections and a durable cutting edge
  • Section pick-up is facilitated as the boat is horizontal allowing the water to completely fill the boat all the way round
  • A hydrophilic surface makes it easy to wet the cutting edge, even with low water level

 

Histology Sectioning: Crucial for medical diagnostics and research, and requires the use of highly precise diamond blades for proper sectioning. Tailored for preparing semi-thin sections suitable for light microscopy, which requires thicker sections in comparison to TEM. 

  • High quality diamonds guarantee perfect sections and a durable cutting edge
  • Easy wetting cutting edge
  • Optimised serial sectioning
  • Large boat for easy pick-up

Cryo Sectioning: Thinner, perfect cryosections of frozen sections of biological specimens, including tissues, cells, or even entire organisms from ultra to semi-thin with the same knife.

  • Thinner cryo sections
  • Perfect cryosections from ultrathin to semi with the same knife
  • Minimal compression and best structure preservation
  • Highest quality diamonds and optimal crystal orientation guarantee perfect ultra thin sections and a durable cutting edge

Ultra Sonic: Patented ultra sonic knife allows the cutting of ultra thin sections free of compression for better structural preservation.

Tested with the following samples:

  • Biological samples in Epon, Araldite, EM Bed, etc.
  • Biological samples in acrylic resins (Lowicryl, LR White).
  • Rigid polymers such as PS, PMMA, ABS, HIPS, modified PP, blends of various kind.

Trim: One trimming knife for all of your trimming needs including at cryo temperatures. The trim series supercedes the cryotrim and ultratrim lines of trimming tools. Resharpening available.

  • Quick, easy and accurate trimming at both room and cryo-temperatures
  • Very shiny sample faces and precise sides are the result
  • Less mechanical damage is applied to the sample during trimming

Static Line II: Neutralise the electrostatic charging in the cryochamber. Sections no longer stick to the knife edge, or bunch up one on top of the other, but float in a nice ribbon over the knife surface. 

Resharpening: Meticulously restoring the knife's cutting edge to its optimal sharpness. DiATOME diamond knives can be resharpened an unlimited amount of times without affecting the cutting-edge length.

  

See the full DiATOME range or select below:

TOOL Diatome Diamond Knives

Diatome Diamond Knives for Ultramicrotomy and Electron Microscopy
Discover precision cutting tools with Diatome Diamond Knives, designed for ultrathin sectioning in electron microscopy (TEM, SEM) and other high-resolution imaging techniques. Available in various models like Ultra 35°, Histo 45°, and Cryo, these knives offer unmatched sharpness, durability, and reliable performance for biological and material sciences. With applications in ambient and cryo-sectioning, Diatome Diamond Knives ensure clean, consistent cuts, making them indispensable for research labs and clinical environments.

Code Title Option 1 Option 2 Option 3 Availability* Price
UCD25-30 Diamond knife, Cryo 25, dry 3.0mm New Each 4 weeks Quote only Quote
UCD25-30R Diamond knife, Cryo 25, dry 3.0mm Resharpened Each 4 weeks Quote only Quote
UCD35-15 Diamond knife, Cryo 35, dry 1.5mm New Each 4 weeks Quote only Quote
UCD35-15E Diamond knife, Cryo 35, dry 1.5mm Exchanged Each 4 weeks Quote only Quote
UCD35-15R Diamond knife, Cryo 35, dry 1.5mm Resharpened Each 4 weeks Quote only Quote
UCD35-18 Diamond knife, Cryo 35, dry 1.8mm New Each 4 weeks Quote only Quote
UCD35-18E Diamond knife, Cryo 35, dry 1.8mm Exchanged Each 4 weeks Quote only Quote
UCD35-18R Diamond knife, Cryo 35, dry 1.8mm Resharpened Each 4 weeks Quote only Quote
UCD35-25 Diamond knife, Cryo 35, dry 2.5mm New Each 4 weeks Quote only Quote
UCD35-25E Diamond knife, Cryo 35, dry 2.5mm Exchanged Each 4 weeks Quote only Quote
UCD35-25R Diamond knife, Cryo 35, dry 2.5mm Resharpened Each 4 weeks Quote only Quote
UCD35-30 Diamond knife, Cryo 35, dry 3.0mm New Each 4 weeks Quote only Quote
UCD35-30E Diamond knife, Cryo 35, dry 3.0mm Exchanged Each 4 weeks Quote only Quote
UCD35-30R Diamond knife, Cryo 35, dry 3.0mm Resharpened Each 4 weeks Quote only Quote
UCD35-35 Diamond knife, Cryo 35, dry 3.5mm New Each 4 weeks Quote only Quote
UCD35-35E Diamond knife, Cryo 35, dry 3.5mm Exchanged Each 4 weeks Quote only Quote
UCD35-35R Diamond knife, Cryo 35, dry 3.5mm Resharpened Each 4 weeks Quote only Quote
UCD35-40 Diamond knife, Cryo 35, dry 4.0mm New Each 4 weeks Quote only Quote
UCD35-40E Diamond knife, Cryo 35, dry 4.0mm Exchanged Each 4 weeks Quote only Quote
UCD35-40R Diamond knife, Cryo 35, dry 4.0mm Resharpened Each 4 weeks Quote only Quote
UCW35-40R Diamond knife, Cryo 35, wet 4.0mm Resharpened Each 4 weeks Quote only Quote
UCW35-15 Diamond knife, Cryo 35, wet 1.5mm New Each 4 weeks Quote only Quote
UCW35-15E Diamond knife, Cryo 35, wet 1.5mm Exchanged Each 4 weeks Quote only Quote
UCW35-15R Diamond knife, Cryo 35, wet 1.5mm Resharpened Each 4 weeks Quote only Quote
UCW35-18 Diamond knife, Cryo 35, wet 1.8mm New Each 4 weeks Quote only Quote
UCW35-18E Diamond knife, Cryo 35, wet 1.8mm Exchanged Each 4 weeks Quote only Quote
UCW35-18R Diamond knife, Cryo 35, wet 1.8mm Resharpened Each 4 weeks Quote only Quote
UCW35-25 Diamond knife, Cryo 35, wet 2.5mm New Each 4 weeks Quote only Quote
UCW35-25E Diamond knife, Cryo 35, wet 2.5mm Exchanged Each 4 weeks Quote only Quote
UCW35-25R Diamond knife, Cryo 35, wet 2.5mm Resharpened Each 4 weeks Quote only Quote
UCW35-30 Diamond knife, Cryo 35, wet 3.0mm New Each 4 weeks Quote only Quote
UCW35-30E Diamond knife, Cryo 35, wet 3.0mm Exchanged Each 4 weeks Quote only Quote
UCW35-30R Diamond knife, Cryo 35, wet 3.0mm Resharpened Each 4 weeks Quote only Quote
UCW35-35 Diamond knife, Cryo 35, wet 3.5mm New Each 4 weeks Quote only Quote
UCW35-35E Diamond knife, Cryo 35, wet 3.5mm Exchanged Each 4 weeks Quote only Quote
UCW35-35R Diamond knife, Cryo 35, wet 3.5mm Resharpened Each 4 weeks Quote only Quote
UCW35-40 Diamond knife, Cryo 35, wet 4.0mm New Each 4 weeks Quote only Quote
UCW35-40E Diamond knife, Cryo 35, wet 4.0mm Exchanged Each 4 weeks Quote only Quote
UCD45-15 Diamond knife, Cryo 45, dry 1.5mm New Each 4 weeks Quote only Quote
UCD45-15E Diamond knife, Cryo 45, dry 1.5mm Exchanged Each 4 weeks Quote only Quote
UCD45-15R Diamond knife, Cryo 45, dry 1.5mm Resharpened Each 4 weeks Quote only Quote
UCD45-25 Diamond knife, Cryo 45, dry 2.5mm New Each 4 weeks Quote only Quote
UCD45-25E Diamond knife, Cryo 45, dry 2.5mm Exchanged Each 4 weeks Quote only Quote
UCD45-25R Diamond knife, Cryo 45, dry 2.5mm Resharpened Each 4 weeks Quote only Quote
UCD45-30 Diamond knife, Cryo 45, dry 3.0mm New Each 4 weeks Quote only Quote
UCD45-30E Diamond knife, Cryo 45, dry 3.0mm Exchanged Each 4 weeks Quote only Quote
UCD45-30R Diamond knife, Cryo 45, dry 3.0mm Resharpened Each 4 weeks Quote only Quote
UCD45-35 Diamond knife, Cryo 45, dry 3.5mm New Each 4 weeks Quote only Quote
UCD45-35E Diamond knife, Cryo 45, dry 3.5mm Exchanged Each 4 weeks Quote only Quote
UCD45-35R Diamond knife, Cryo 45, dry 3.5mm Resharpened Each 4 weeks Quote only Quote
UCD45-40 Diamond knife, Cryo 45, dry 4.0mm New Each 4 weeks Quote only Quote
UCD45-40E Diamond knife, Cryo 45, dry 4.0mm Exchanged Each 4 weeks Quote only Quote
UCD45-40R Diamond knife, Cryo 45, dry 4.0mm Resharpened Each 4 weeks Quote only Quote
UCW45-15 Diamond knife, Cryo 45, wet 1.5mm New Each 4 weeks Quote only Quote
UCW45-15E Diamond knife, Cryo 45, wet 1.5mm Exchanged Each 4 weeks Quote only Quote
UCW45-15R Diamond knife, Cryo 45, wet 1.5mm Resharpened Each 4 weeks Quote only Quote
UCW45-25 Diamond knife, Cryo 45, wet 2.5mm New Each 4 weeks Quote only Quote
UCW45-25E Diamond knife, Cryo 45, wet 2.5mm Exchanged Each 4 weeks Quote only Quote
UCW45-25R Diamond knife, Cryo 45, wet 2.5mm Resharpened Each 4 weeks Quote only Quote
UCW45-30 Diamond knife, Cryo 45, wet 3.0mm New Each 4 weeks Quote only Quote
UCW45-30E Diamond knife, Cryo 45, wet 3.0mm Exchanged Each 4 weeks Quote only Quote
UCW45-30R Diamond knife, Cryo 45, wet 3.0mm Resharpened Each 4 weeks Quote only Quote
UCW45-35 Diamond knife, Cryo 45, wet 3.5mm New Each 4 weeks Quote only Quote
UCW45-35E Diamond knife, Cryo 45, wet 3.5mm Exchanged Each 4 weeks Quote only Quote
UCW45-35R Diamond knife, Cryo 45, wet 3.5mm Resharpened Each 4 weeks Quote only Quote
UCW45-40 Diamond knife, Cryo 45, wet 4.0mm New Each 4 weeks Quote only Quote
UCW45-40E Diamond knife, Cryo 45, wet 4.0mm Exchanged Each 4 weeks Quote only Quote
UCW45-40R Diamond knife, Cryo 45, wet 4.0mm Resharpened Each 4 weeks Quote only Quote
UCA35-20 Diamond knife, Cryo AFM 35 2.0mm New Each 4 weeks Quote only Quote
UCA35-20R Diamond knife, Cryo AFM 35 2.0mm resharpened Each 4 weeks Quote only Quote
UCA35-30 Diamond knife, Cryo AFM 35 3.0mm New Each 4 weeks Quote only Quote
UCA35-30R Diamond knife, Cryo AFM 35 3.0mm resharpened Each 4 weeks Quote only Quote
UCI35-20 Diamond knife, Cryo Immuno 35 2.0mm New Each 4 weeks Quote only Quote
UCI35-20R Diamond knife, Cryo Immuno 35 2.0mm Resharpened Each 4 weeks Quote only Quote
UCI35-30 Diamond knife, Cryo Immuno 35 3.0mm New Each 4 weeks Quote only Quote
UCI35-30R Diamond knife, Cryo Immuno 35 3.0mm Resharpened Each 4 weeks Quote only Quote
UH45-40 Diamond knife, Histo 45 4.0mm New Each 4 weeks Quote only Quote
UH45-40E Diamond knife, Histo 45 4.0mm Exchanged Each 4 weeks Quote only Quote
UH45-40R Diamond knife, Histo 45 4.0mm Resharpened Each 4 weeks Quote only Quote
UH45-60 Diamond knife, Histo 45 6.0mm New Each 4 weeks Quote only Quote
UH45-60E Diamond knife, Histo 45 6.0mm Exchanged Each 4 weeks Quote only Quote
UH45-60R Diamond knife, Histo 45 6.0mm Resharpened Each 4 weeks Quote only Quote
UH45-80 Diamond knife, Histo 45 8.0mm New Each 4 weeks Quote only Quote
UH45-80E Diamond knife, Histo 45 8.0mm Exchanged Each 4 weeks Quote only Quote
UH45-80R Diamond knife, Histo 45 8.0mm Resharpened Each 4 weeks Quote only Quote
UHCD45-40 Diamond knife, Histo Cryo 45, dry 4.0mm New Each 4 weeks Quote only Quote
UHCD45-40R Diamond knife, Histo Cryo 45, dry 4.0mm Resharpened Each 4 weeks AU $1,956.66
UHCD45-60 Diamond knife, Histo Cryo 45, dry 6.0mm New Each 4 weeks Quote only Quote
UHCD45-60R Diamond knife, Histo Cryo 45, dry 6.0mm Resharpened Each 4 weeks Quote only Quote
UHCW45-40 Diamond knife, Histo Cryo 45, wet 4.0mm New Each 4 weeks Quote only Quote
UHCW45-40R Diamond knife, Histo Cryo 45, wet 4.0mm Resharpened Each 4 weeks AU $1,956.66
UHCW45-60 Diamond knife, Histo Cryo 45, wet 6.0mm New Each 4 weeks Quote only Quote
UHCW45-60R Diamond knife, Histo Cryo 45, wet 6.0mm Resharpened Each 4 weeks Quote only Quote
UHJ45-60 Diamond knife, Histo Jumbo 45 6.0mm New Each 4 weeks Quote only Quote
UHJ45-60E Diamond knife, Histo Jumbo 45 6.0mm Exchanged Each 4 weeks Quote only Quote
UHJ45-60R Diamond knife, Histo Jumbo 45 6.0mm Resharpened Each 4 weeks Quote only Quote
UHJ45-80 Diamond knife, Histo Jumbo 45 8.0mm New Each 4 weeks Quote only Quote
UHJ45-80E Diamond knife, Histo Jumbo 45 8.0mm Exchanged Each 4 weeks Quote only Quote
UHJ45-80R Diamond knife, Histo Jumbo 45 8.0mm Resharpened Each 4 weeks Quote only Quote
UU35-15 Diamond knife, Ultra 35 1.5mm New Each 4 weeks Quote only Quote
UU35-15E Diamond knife, Ultra 35 1.5mm Exchanged Each 4 weeks Quote only Quote
UU35-15R Diamond knife, Ultra 35 1.5mm Resharpened Each 4 weeks Quote only Quote
UU35-25 Diamond knife, Ultra 35 2.5mm New Each 4 weeks Quote only Quote
UU35-25E Diamond knife, Ultra 35 2.5mm Exchanged Each 4 weeks Quote only Quote
UU35-25R Diamond knife, Ultra 35 2.5mm Resharpened Each 4 weeks Quote only Quote
UU35-30 Diamond knife, Ultra 35 3.0mm New Each 4 weeks Quote only Quote
UU35-30E Diamond knife, Ultra 35 3.0mm Exchanged Each 4 weeks Quote only Quote
UU35-30R Diamond knife, Ultra 35 3.0mm Resharpened Each 4 weeks Quote only Quote
UU35-35 Diamond knife, Ultra 35 3.5mm New Each 4 weeks Quote only Quote
UU35-35E Diamond knife, Ultra 35 3.5mm Exchanged Each 4 weeks Quote only Quote
UU35-35R Diamond knife, Ultra 35 3.5mm Resharpened Each 4 weeks Quote only Quote
UU35-40 Diamond knife, Ultra 35 4.0mm New Each 4 weeks Quote only Quote
UU35-40E Diamond knife, Ultra 35 4.0mm Exchanged Each 4 weeks Quote only Quote
UU35-40R Diamond knife, Ultra 35 4.0mm Resharpened Each 4 weeks Quote only Quote
UU45-15 Diamond knife, Ultra 45 1.5mm New Each 4 weeks Quote only Quote
UU45-15E Diamond knife, Ultra 45 1.5mm Exchanged Each 4 weeks Quote only Quote
UU45-15R Diamond knife, Ultra 45 1.5mm Resharpened Each 4 weeks Quote only Quote
UU45-25 Diamond knife, Ultra 45 2.5mm New Each 4 weeks Quote only Quote
UU45-25E Diamond knife, Ultra 45 2.5mm Exchanged Each 4 weeks Quote only Quote
UU45-25R Diamond knife, Ultra 45 2.5mm Resharpened Each 4 weeks Quote only Quote
UU45-30 Diamond knife, Ultra 45 3.0mm New Each 4 weeks Quote only Quote
UU45-30E Diamond knife, Ultra 45 3.0mm Exchanged Each 4 weeks Quote only Quote
UU45-30R Diamond knife, Ultra 45 3.0mm Resharpened Each 4 weeks Quote only Quote
UU45-40 Diamond knife, Ultra 45 4.0mm New Each 4 weeks Quote only Quote
UU45-40E Diamond knife, Ultra 45 4.0mm Exchanged Each 4 weeks Quote only Quote
UU45-35 Diamond knife, Ultra 45 3.5mm New Each 4 weeks Quote only Quote
UU45-35E Diamond knife, Ultra 45 3.5mm Exchanged Each 4 weeks Quote only Quote
UU45-35R Diamond knife, Ultra 45 3.5mm Resharpened Each 4 weeks Quote only Quote
UU45-40R Diamond knife, Ultra 45 4.0mm Resharpened Each 4 weeks Quote only Quote
UU45-20 Diamond knife, Ultra 45 2.0mm New Each 4 weeks Quote only Quote
UU45-20E Diamond knife, Ultra 45 2.0mm Exchanged Each 4 weeks Quote only Quote
UU45-20R Diamond knife, Ultra 45 2.0mm Resharpened Each 4 weeks Quote only Quote
UUA35-20 Diamond knife, Ultra AFM 35 2.0mm New Each 4 weeks Quote only Quote
UUA35-20R Diamond knife, Ultra AFM 35 2.0mm Resharpened Each 4 weeks Quote only Quote
UUA35-30 Diamond knife, Ultra AFM 35 3.0mm New Each 4 weeks Quote only Quote
UUA35-30R Diamond knife, Ultra AFM 35 3.0mm Resharpened Each 4 weeks Quote only Quote
DIA30-UL-ATS Diamond knife, Ultra ATS 3.0mm New Each 6 weeks Quote only Quote
UUJ35-20 Diamond knife, Ultra Jumbo 35 2.0mm New Each 4 weeks Quote only Quote
UUJ35-20R Diamond knife, Ultra Jumbo 35 2.0mm Resharpened Each 4 weeks Quote only Quote
UUJ35-30 Diamond knife, Ultra Jumbo 35 3.0mm New Each 4 weeks Quote only Quote
UUJ35-30R Diamond knife, Ultra Jumbo 35 3.0mm Resharpened Each 4 weeks Quote only Quote
UUJ45-20 Diamond knife, Ultra Jumbo 45 2.0mm New Each 4 weeks Quote only Quote
UUJ45-20R Diamond knife, Ultra Jumbo 45 2.0mm Resharpened Each 4 weeks Quote only Quote
UUJ45-30 Diamond knife, Ultra Jumbo 45 3.0mm New Each 4 weeks Quote only Quote
UUJ45-30R Diamond knife, Ultra Jumbo 45 3.0mm Resharpened Each 4 weeks Quote only Quote
UUS35-30 Diamond knife, Ultra Sonic 35 Knife only New Each 4 weeks Quote only Quote
UUS35-30CU Diamond knife, Ultra Sonic 35 Knife & control unit New Each 4 weeks Quote only Quote
UUS35-30R Diamond knife, Ultra Sonic 35 Knife only Resharpened Each 4 weeks Quote only Quote
UUS30-ULTSONM Diamond knife, Ultra Sonic Maxi 35 3mm knife, control unit New Each 0 in stock (?) Quote only Quote
UUS30-ULTSONMK Diamond knife, Ultra Sonic Maxi 35 3mm knife New Each 0 in stock (?) Quote only Quote
UTT20 DiATOME Trimtool 20 deg. New Each 4 weeks Quote only Quote
UTT20-R DiATOME Trimtool 20 deg. Resharpened Each 4 weeks Quote only Quote
UTT45 DiATOME Trimtool 45 deg. New Each 4 weeks Quote only Quote
UTT45-R DiATOME Trimtool 45 deg. Resharpened Each 4 weeks Quote only Quote
UTT90 DiATOME Trimtool 90 deg. New Each 4 weeks Quote only Quote
UTT90-R DiATOME Trimtool 90 deg. Resharpened Each 4 weeks Quote only Quote