Ultra Small ImmunoGold Reagents

Aurion Ultra Small Immunogold Reagents are prepared with subnanometer gold particles. These particles have far less influence on the adsorbed antibodies or detecting molecules, and consequently the conjugates behave as though they are uncoupled. In conjunction with the highly efficient and easy-to-use R-Gent SE-LM and SE-EM silver enhancement reagents, the Ultra Small Immunogold Reagents are the best choice for any application.

Introduction

Reduction of the gold particle size provides Ultra Small Immunogold Reagents with fundamentally different characteristics when compared with conjugates built around larger particles. While the Conventional Immunogold Reagents can be thought of as particles coated with proteins, Ultra Small Immunogold Reagents are proteins coated with one or more gold particles. With this structure, both the overall size of the conjugates, as well as steric hindrance are decreased. The table below lists a few physical characteristics of gold conjugates.

Aurion offers Ultra Small Immunogold Reagents with an average gold particle diameter of 0.8 nm or less. These ultra small gold particles can be visualized directly in high angle annular dark-field-scanning TEM. However, the gold signal is normally visualized after increasing the particle diameter with silver enhancement. The reagents can be used in electron and light microscopy as well as in blotting experiments. The universal applicability makes it easy to compare results obtained with different procedures.

Product Description

AURION Ultra Small Immunogold Reagents contain 60-80 μg of specific protein/ml for IgG conjugates. F(ab')2, Fab and biotinylated albumin conjugates contain equimolar amounts of conjugated protein. The average gold cluster diameter is less than 0.8 nm. AURION Ultra Small Immunogold Reagents are used in conjunction with AURION R-Gent SE-EM or SE-LM silver enhancement reagents, developed for electron microscopy and light microscopy/immunoblotting respectively. The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353. The products are available in two package sizes: Regular package: for 1200 grids or 600 slides, Small package: for 480 grids or 240 slides.

Specificity

Aurion offers the widest range of Ultra Small Immunogold Reagents. The most commonly used reagent types are available as intact IgG, F(ab')2 and Single Fab conjugates. To view the complete list of available reagents click here. AURION Ultra Small Immunogold Reagents are prepared using the highest quality antibodies or binding agents available. All antibodies are immuno affinity purified and immuno cross-adsorbed to reduce nonspecific interactions. Storage AURION Immunogold Reagents have a guaranteed shelf life of 18 months from the date of quality control analysis.

The products should be stored at 4-8°C. Freezing is not recommended.


Products with Ultra Small option variants are listed below. 

*Use the toggle in the option 1 column to review ultra small options together. 

 

Code Title Option 1 Option 2 Option 3 Availability* Price
EMS25386 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 15nm 3.5ml 0 in stock (?) AU $929.00
JA800-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade UltraSmall 0.6ml 0 in stock (?) AU $460.00
JA100-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade UltraSmall 1.5ml 0 in stock (?) AU $789.00
JA806-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 6nm 1ml 0 in stock (?) AU $460.00
JA106-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 6nm 2.5ml 0 in stock (?) AU $746.00
JA810-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 10nm 1ml 0 in stock (?) AU $460.00
JA110-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 10nm 2.5ml 0 in stock (?) AU $789.00
JA815-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 15nm 1.4ml 0 in stock (?) AU $460.00
JA825-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 25nm 1.4ml 0 in stock (?) AU $460.00
JA125-188 Aurion F(ab')2 frag of goat anti-mouse IgG+IgM EM-grade 25nm 3.5ml 0 in stock (?) AU $789.00
JB15850 BBI bovine serum albumin, EM-grade 5nm 0.25ml 3 weeks AU $348.00
JB15851 BBI bovine serum albumin, EM-grade 10nm 0.25ml 3 weeks AU $437.00
JB15851-1 BBI bovine serum albumin, EM-grade 10nm 1ml 3 weeks AU $1,039.00
JA800-099 Aurion streptavidin, EM-grade UltraSmall 0.6ml 0 in stock (?) AU $458.00
JA100-099 Aurion streptavidin, EM-grade UltraSmall 1.5ml 0 in stock (?) AU $717.00
JA806-099 Aurion streptavidin, EM-grade 6nm 1ml 0 in stock (?) AU $458.00
JA106-099 Aurion streptavidin, EM-grade 6nm 2.5ml 0 in stock (?) AU $717.00
JA810-099 Aurion streptavidin, EM-grade 10nm 1ml 0 in stock (?) AU $458.00
JA110-099 Aurion streptavidin, EM-grade 10nm 2.5ml 0 in stock (?) AU $717.00
JA815-099 Aurion streptavidin, EM-grade 15nm 1.4ml 0 in stock (?) AU $458.00
JA115-099 Aurion streptavidin, EM-grade 15nm 3.5ml 0 in stock (?) AU $717.00
JA825-099 Aurion streptavidin, EM-grade 25nm 1.4ml 0 in stock (?) AU $458.00
JA125-099 Aurion streptavidin, EM-grade 25nm 3.5ml 0 in stock (?) AU $717.00
JA800-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade UltraSmall 0.6ml 0 in stock (?) AU $460.00
JA100-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade UltraSmall 1.5ml 0 in stock (?) AU $789.00
JA806-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 6nm 1ml 0 in stock (?) AU $460.00
JA106-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 6nm 2.5ml 0 in stock (?) AU $789.00
JA810-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 10nm 1ml 0 in stock (?) AU $460.00
JA110-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 10nm 2.5ml 0 in stock (?) AU $789.00
JA815-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 15nm 1.4ml 0 in stock (?) AU $460.00
JA115-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 15nm 3.5ml 0 in stock (?) AU $789.00
JA825-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 25nm 1.4ml 0 in stock (?) AU $460.00
JA125-155 Aurion rabbit anti-sheep IgG (H&L), EM-grade 25nm 3.5ml 0 in stock (?) AU $789.00
JA800-077 Aurion rabbit anti-goat IgG (H&L), EM-grade UltraSmall 0.6ml 0 in stock (?) AU $458.00
JA100-077 Aurion rabbit anti-goat IgG (H&L), EM-grade UltraSmall 1.5ml 0 in stock (?) AU $717.00
JA806-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 6nm 1ml 0 in stock (?) AU $458.00
JA106-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 6nm 2.5ml 0 in stock (?) AU $717.00
JA810-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 10nm 1ml 0 in stock (?) AU $458.00
JA110-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 10nm 2.5ml 0 in stock (?) AU $717.00
JA815-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 15nm 1.4ml 0 in stock (?) AU $458.00
JA115-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 15nm 3.5ml 0 in stock (?) AU $717.00
JA825-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 25nm 1.4ml 0 in stock (?) AU $458.00
JA125-077 Aurion rabbit anti-goat IgG (H&L), EM-grade 25nm 3.5ml 0 in stock (?) AU $717.00
JA800-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade UltraSmall 0.6ml 0 in stock (?) AU $460.00
JA100-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade UltraSmall 1.5ml 0 in stock (?) AU $789.00
JA806-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade 6nm 1ml 0 in stock (?) AU $460.00
JA106-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade 6nm 2.5ml 0 in stock (?) AU $789.00
JA810-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade 10nm 1ml 0 in stock (?) AU $460.00
JA110-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade 10nm 2.5ml 0 in stock (?) AU $789.00
JA815-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade 15nm 1.4ml 0 in stock (?) AU $460.00
JA115-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade 15nm 3.5ml 0 in stock (?) AU $789.00
EMS25436 Aurion rabbit anti-chicken IgG (H&L) EM-grade 25nm 1.4ml 0 in stock (?) AU $460.00
JA125-222 Aurion rabbit anti-chicken IgG (H&L) EM-grade 25nm 3.5ml 0 in stock (?) AU $789.00
JA800-122 Aurion biotinylated albumin, EM-grade UltraSmall 0.6ml 0 in stock (?) AU $458.00
JA100-122 Aurion biotinylated albumin, EM-grade UltraSmall 1.5ml 0 in stock (?) AU $717.00
JA806-122 Aurion biotinylated albumin, EM-grade 6nm 1ml 0 in stock (?) AU $437.00
JA106-122 Aurion biotinylated albumin, EM-grade 6nm 2.5ml 0 in stock (?) AU $683.00
JA110-122 Aurion biotinylated albumin, EM-grade 10nm 2.5ml 0 in stock (?) AU $717.00
JA810-122 Aurion biotinylated albumin, EM-grade 10nm 1ml 0 in stock (?) AU $458.00
JA815-122 Aurion biotinylated albumin, EM-grade 15nm 1.4ml 0 in stock (?) AU $437.00
JA115-122 Aurion biotinylated albumin, EM-grade 15nm 3.5ml 0 in stock (?) AU $683.00
JA825-122 Aurion biotinylated albumin, EM-grade 25nm 1.4ml 0 in stock (?) AU $437.00
JA125-122 Aurion biotinylated albumin, EM-grade 25nm 3.5ml 0 in stock (?) AU $683.00
JA800-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade UltraSmall 0.6ml 0 in stock (?) AU $460.00
JA100-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade UltraSmall 1.5ml 0 in stock (?) AU $789.00
JA806-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 6nm 1ml 0 in stock (?) AU $460.00
JA106-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 6nm 2.5ml 0 in stock (?) AU $789.00
JA810-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 10nm 1ml 0 in stock (?) AU $460.00
JA110-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 10nm 2.5ml 0 in stock (?) AU $789.00
JA815-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 15nm 1.4ml 0 in stock (?) AU $460.00
JA115-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 15nm 3.5ml 0 in stock (?) AU $789.00
JA825-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 25nm 1.4ml 0 in stock (?) AU $460.00
JA125-177 Aurion F(ab')2 frag of goat anti-mouse IgG (H&L) EM-grade 25nm 3.5ml 0 in stock (?) AU $789.00
JA800-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade UltraSmall 0.6ml 0 in stock (?) AU $460.00
JA100-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade UltraSmall 1.5ml 0 in stock (?) AU $789.00
JA806-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 6nm 1ml 0 in stock (?) AU $460.00
JA106-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 6nm 2.5ml 0 in stock (?) AU $789.00
JA810-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 10nm 1ml 0 in stock (?) AU $460.00
JA110-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 10nm 2.5ml 0 in stock (?) AU $789.00
JA815-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 15nm 1.4ml 0 in stock (?) AU $460.00
JA115-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 15nm 3.5ml 0 in stock (?) AU $789.00
JA825-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 25nm 1.4ml 0 in stock (?) AU $460.00
JA125-166 Aurion F(ab')2 frag of goat anti-rabbit IgG (H&L) EM-grade 25nm 3.5ml 0 in stock (?) AU $789.00
JA800-211 Aurion F(ab) frag of sheep anti-digoxigenin UltraSmall 0.6ml 0 in stock (?) AU $460.00
JA100-211 Aurion F(ab) frag of sheep anti-digoxigenin UltraSmall 1.5ml 0 in stock (?) AU $751.00
JA806-199 Aurion F(ab) frag of sheep anti-digoxigenin 6nm 1ml 0 in stock (?) AU $439.00
JA106-199 Aurion F(ab) frag of sheep anti-digoxigenin 6nm 2.5ml 0 in stock (?) AU $751.00
JA810-199 Aurion F(ab) frag of sheep anti-digoxigenin 10nm 1ml 0 in stock (?) AU $460.00
JA110-199 Aurion F(ab) frag of sheep anti-digoxigenin 10nm 2.5ml 0 in stock (?) AU $789.00
JA815-199 Aurion F(ab) frag of sheep anti-digoxigenin 15nm 1.4ml 0 in stock (?) AU $439.00
JA115-199 Aurion F(ab) frag of sheep anti-digoxigenin 15nm 3.5ml 0 in stock (?) AU $713.00
JA825-199 Aurion F(ab) frag of sheep anti-digoxigenin 25nm 1.4ml 0 in stock (?) AU $439.00
JA125-199 Aurion F(ab) frag of sheep anti-digoxigenin 25nm 3.5ml 0 in stock (?) AU $713.00
JA800-088 Aurion goat anti-biotin, EM-grade UltraSmall 0.6ml 0 in stock (?) AU $460.00
JA100-088 Aurion goat anti-biotin, EM-grade UltraSmall 1.5ml 0 in stock (?) AU $751.00
JA806-088 Aurion goat anti-biotin, EM-grade 6nm 1ml 0 in stock (?) AU $460.00
JA106-088 Aurion goat anti-biotin, EM-grade 6nm 2.5ml 0 in stock (?) AU $751.00
JA810-088 Aurion goat anti-biotin, EM-grade 10nm 1ml 0 in stock (?) AU $460.00
JA110-088 Aurion goat anti-biotin, EM-grade 10nm 2.5ml 0 in stock (?) AU $751.00
JA815-088 Aurion goat anti-biotin, EM-grade 15nm 1.4ml 0 in stock (?) AU $460.00
JA115-088 Aurion goat anti-biotin, EM-grade 15nm 3.5ml 0 in stock (?) AU $751.00
JA825-088 Aurion goat anti-biotin, EM-grade 25nm 1.4ml 0 in stock (?) AU $460.00
JA125-088 Aurion goat anti-biotin, EM-grade 25nm 3.5ml 0 in stock (?) AU $751.00
JA800-244 Aurion goat anti-chicken IgG (H&L), EM-grade UltraSmall 0.6ml 0 in stock (?) AU $496.00
JA100-244 Aurion goat anti-chicken IgG (H&L), EM-grade UltraSmall 1.5ml 0 in stock (?) AU $948.00
JA806-244 Aurion goat anti-chicken IgG (H&L), EM-grade 6nm 1ml 0 in stock (?) AU $496.00
JA106-244 Aurion goat anti-chicken IgG (H&L), EM-grade 6nm 2.5ml 0 in stock (?) AU $800.00
JA810-244 Aurion goat anti-chicken IgG (H&L), EM-grade 10nm 1ml 0 in stock (?) AU $496.00
JA110-244 Aurion goat anti-chicken IgG (H&L), EM-grade 10nm 2.5ml 0 in stock (?) AU $800.00
JA815-244 Aurion goat anti-chicken IgG (H&L), EM-grade 15nm 1.4ml 0 in stock (?) AU $496.00
JA115-244 Aurion goat anti-chicken IgG (H&L), EM-grade 15nm 3.5ml 0 in stock (?) AU $800.00
JA825-244 Aurion goat anti-chicken IgG (H&L), EM-grade 25nm 1.4ml 0 in stock (?) AU $496.00
JA125-244 Aurion goat anti-chicken IgG (H&L), EM-grade 25nm 3.5ml 0 in stock (?) AU $800.00