A buffer is a solution that can resist pH change with the addition of an acid or base. It is able to neutralise small amounts of added acid or base, thus maintaining the pH of the solution relatively stable. This is important for processes and or reactions which require specific and stable pH ranges.
Buffers generally consist of a weak acid and its salt or a weak base and its salt. The solutions resist pH change because of a chemical equilibrium between the weak acid HA and its conjugate base A−:
HA ⇌ H+ + A−
Buffers are used with fixatives which require certain properties:
1. A constant pH during the time of the fixation (The pH of the fixative in electron microscopy is adjusted using the buffer system chosen)
2. Prevention of the extraction of cellular components, or the deposition of precipitates of fixative chemicals.
A wide range of buffer solutions and powders are available, suitable for most laboratory applications.
CHEM Buffers
| Code | Title | Option 1 | Option 2 | Option 3 | Availability* | Price | |
|---|---|---|---|---|---|---|---|
| EMS26671-02 | Acetate buffer (DG) | 6.5-8.0 | 500ml | 2 weeks | AU $80.00 | ||
| EMS11480 | Acetate buffer 0.2M (DG) | 3.5 - 5.6 | 225ml | leadtime:4-6 weeks | AU $84.00 | ||
| EMS11482 | Acetate buffer 0.2M (DG) | 3.5 - 5.6 | 500ml | leadtime:4-6 weeks | AU $126.00 | ||
| EMS11482-42 | Acetate buffer 0.2M (DG) | 4.2 | 500ml | leadtime:4-6 weeks | AU $126.00 | ||
| EMS11482-50 | Acetate buffer 0.2M (DG) | 5 | 500ml | leadtime:4-6 weeks | AU $126.00 | ||
| EMS11482-56 | Acetate buffer 0.2M (DG) | 5.6 | 500ml | leadtime:4-6 weeks | AU $126.00 | ||
| EMS11455-78 | Borate buffer | pH 7.8 | 500ml | 2 weeks | AU $97.00 | ||
| EMS11455-82 | Borate buffer | pH 8.2 | 500ml | 2 weeks | AU $103.00 | ||
| EMS11455-90 | Borate buffer | pH 9.0 | 500ml | 2 weeks | AU $103.00 | ||
| EMS26303-03 | Borate buffer 0.1M | 9 | 500ml | 2 weeks | AU $80.00 | ||
| EMS64142-07 | Citrate buffer | 6 | 100ml | 2 weeks | AU $176.00 | ||
| EMS64142-08 | Citrate buffer | 6 | 1L | 2 weeks | AU $295.00 | ||
| EMS11490 | Citrate buffer 0.1M | 4.6-6.2 | 225ml | 2 weeks | AU $57.00 | ||
| EMS11492 | Citrate buffer 0.1M | 4.6-6.2 | 500ml | 2 weeks | AU $75.00 | ||
| EMS11492-04 | Citrate Buffer 0.2M | pH 4.0 | 1L | 2 weeks | AU $130.00 | ||
| EMS11492-06 | Citrate Buffer 0.2M | pH 6.0 | 1L | 2 weeks | AU $134.00 | ||
| EMS11772-05 | D-Phosphate buffer saline formula | Formula I, 0.15M | 500ml | 2 weeks | AU $83.00 | ||
| EMS11772-10 | D-Phosphate buffer saline formula | Formula I, 0.15M | 1L | 2 weeks | AU $108.00 | ||
| EMS11773-05 | D-Phosphate buffer saline formula | Formula II, 0.19M | 500ml | 2 weeks | AU $83.00 | ||
| EMS11773-10 | D-Phosphate buffer saline formula | Formula II, 0.19M | 1L | 2 weeks | AU $111.00 | ||
| EMS11774-05 | D-Phosphate buffer saline formula | Formula III, 0.15M | 500ml | 2 weeks | AU $92.00 | ||
| EMS11774-10 | D-Phosphate buffer saline formula | Formula III, 0.15M | 1L | 2 weeks | AU $118.00 | ||
| EMS64142-09 | EDTA buffer (10X) | 8.5 | 100ml | 2 weeks | AU $176.00 | ||
| EMS64142-10 | EDTA buffer (10X) | 8.5 | 1L | 2 weeks | AU $295.00 | ||
| EMS16530 | Glutaraldehyde 10% in solvent (DG) | Acetone | 10 x 10ml | 2 weeks | AU $200.00 | ||
| EMS16531 | Glutaraldehyde 10% in solvent (DG) | Ethanol | 10 x 10ml | 2 weeks | AU $200.00 | ||
| EMS16532 | Glutaraldehyde 10% in solvent (DG) | Methanol | 10 x 10ml | 2 weeks | AU $200.00 | ||
| AHEP68-1L | Hepes buffer solution | 6.8 | 1L | 3 weeks | AU $163.00 | ||
| EMS11494 | Hepes buffer solution, 0.2M | 7.0-8.0 | 500ml | 2 weeks | AU $73.00 | ||
| C018 | Hepes buffer, powder | 7.0-8.0 | 25g | 2 weeks | AU $47.00 | ||
| C0181 | Hepes buffer, powder | 7.0-8.0 | 100g | 2 weeks | AU $124.00 | ||
| EMS18041-05 | Michel's buffer | 7.4 | 500ml | 2 weeks | AU $73.00 | ||
| EMS18041-10 | Michel's buffer | 7.4 | 1L | 2 weeks | AU $108.00 | ||
| EMS11582-05 | Millonig's phosphate buffer, 0.2M | 7.4 | 500ml | 2 weeks | AU $74.00 | ||
| EMS11582-10 | Millonig's phosphate buffer, 0.2M | 7.4 | 1L | 2 weeks | AU $111.00 | ||
| EMS18900 | Mops biological buffer | 6.5-7.9 | 100g | 2 weeks | AU $167.00 | ||
| EMS11162 | PHEM buffer | 0.1M | 225ml | 2 weeks | AU $119.00 | ||
| EMS11163 | PHEM buffer | 0.1M | 500ml | 2 weeks | AU $200.00 | ||
| EMS11164 | PHEM buffer | 0.2M | 225ml | 2 weeks | AU $119.00 | ||
| EMS11165 | PHEM buffer | 0.2M | 500ml | 2 weeks | AU $200.00 | ||
| EMS19340-60 | Phosphate buffer 0.1M (EMS) | 6 | 1L | 2 weeks | AU $125.00 | ||
| EMS19340-65 | Phosphate buffer 0.1M (EMS) | 6.5 | 1L | 2 weeks | AU $125.00 | ||
| EMS19340-70 | Phosphate buffer 0.1M (EMS) | 7 | 1L | 2 weeks | AU $125.00 | ||
| EMS19340-72 | Phosphate buffer 0.1M (EMS) | 7.2 | 1L | 2 weeks | AU $125.00 | ||
| EMS19342-10 | Phosphate buffered saline, 10X (EMS) | 7.4 | 1L | 2 weeks | AU $108.00 | ||
| EMS19342-40 | Phosphate buffered saline, 10X (EMS) | 7.4 | 3785ml | 2 weeks | AU $325.00 | ||
| EMS19344-10 | Phosphate buffered saline, 1X (EMS) | 7.4 | 1L | 2 weeks | AU $95.00 | ||
| EMS19344-20 | Phosphate buffered saline, 1X (EMS) | 7.4 | 3785ml | 2 weeks | AU $284.00 | ||
| EMS19343-10 | Phosphate buffered saline, 2X (EMS) | 7.4 | 1L | 2 weeks | AU $101.00 | ||
| EMS19343-40 | Phosphate buffered saline, 2X (EMS) | 7.4 | 3785ml | 2 weeks | AU $305.00 | ||
| APBS-1L | Phosphate buffered saline, pH 7.4 | 7.4 | 1L | 3 weeks | AU $114.00 | ||
| EMS11610 | Pipes buffer solution 0.3M | 5.5-6.0 | 500ml | 2 weeks | AU $134.00 | ||
| C022 | Pipes buffer, powder | 6.4-7.2 | 25g | 2 weeks | AU $108.00 | ||
| C0221 | Pipes buffer, powder | 6.4-7.2 | 100g | 2 weeks | AU $337.00 | ||
| EMS62706-12 | R-Buffer (DG) for retriever slide stainer | 4.5 (C) | 250ml | 2 weeks | AU $178.00 | ||
| EMS62706-10 | R-Buffers for retriever slide stainer | 6.0 (A) | 250ml | 2 weeks | AU $178.00 | ||
| EMS62706-11 | R-Buffers for retriever slide stainer | 8.0 (B) | 250ml | 2 weeks | AU $178.00 | ||
| EMS62706-13 | R-Buffers for retriever slide stainer | 6.0 (U) | 250ml | 2 weeks | AU $178.00 | ||
| EMS62707-10 | R-Buffers for retriever slide stainer | 6.0 (Ag) | 250ml | 2 weeks | AU $178.00 | ||
| EMS62707-11 | R-Buffers for retriever slide stainer | 8.0 (Bg) | 250ml | 2 weeks | AU $178.00 | ||
| EMS62707-12 | R-Buffers for retriever slide stainer | 4.5 (Cg) | 250ml | 2 weeks | AU $178.00 | ||
| EMS62707-13 | R-Buffers for retriever slide stainer | 6.0 (Ug) | 250ml | 2 weeks | AU $178.00 | ||
| EMS15953 | Sodium cacodylate buffer | 7.4 | 10 x Vials/10ml | 2 weeks | AU $106.00 | ||
| EMS11650 | Sodium cacodylate buffer 0.2M | 7.4 | 225ml | 2 weeks | AU $84.00 | ||
| EMS11652 | Sodium cacodylate buffer 0.2M | 7.4 | 500ml | 2 weeks | AU $120.00 | ||
| EMS11653 | Sodium cacodylate buffer 0.2M | 7.4 | 1L | 2 weeks | AU $215.00 | ||
| EMS11654 | Sodium cacodylate buffer 0.4M | 7.2 | 500ml | 2 weeks | AU $159.00 | ||
| EMS11655 | Sodium cacodylate buffer 0.4M | 7.2 | 1L | 2 weeks | AU $266.00 | ||
| C216 | Sodium hydrogen phosphate, monobasic | 4.1-4.5 | 500g | 2 weeks | AU $103.00 | ||
| EMS11682-05 | Sorensen's phosphate buffer 0.15M | 7.4 | 500ml | 2 weeks | AU $86.00 | ||
| EMS11682-10 | Sorensen's phosphate buffer 0.15M | 7.4 | 1L | 2 weeks | AU $113.00 | ||
| EMS11600-05 | Sorensen's phosphate buffer 0.2M | 7.2 | 500ml | 2 weeks | AU $78.00 | ||
| EMS11600-10 | Sorensen's phosphate buffer 0.2M | 7.2 | 1L | 2 weeks | AU $111.00 | ||
| EMS11600-40 | Sorensen's phosphate buffer 0.2M | 7.2 | 4L | 2 weeks | AU $351.00 | ||
| EMS11601-10 | Sorensen's phosphate buffer 0.2M | 7.4 | 1L | 2 weeks | AU $113.00 | ||
| EMS11601-40 | Sorensen's phosphate buffer 0.2M | 7.4 | 4L | 2 weeks | AU $351.00 | ||
| ASOR68C-1L | Sorensen's phosphate buffer, 2M | 6.8 | 1L | 3 weeks | AU $68.00 | ||
| ASOR68C-5L | Sorensen's phosphate buffer, 2M | 6.8 | 5L | 3 weeks | AU $283.00 | ||
| EMS11730-05 | Tris buffer 0.2M | 8 | 250ml | 2 weeks | AU $69.00 | ||
| EMS11730-06 | Tris buffer 0.2M | 8 | 500ml | 2 weeks | AU $92.00 | ||
| EMS11730-08 | Tris buffered saline, pH 7.4 | 7.4 | 250ml | 2 weeks | AU $69.00 | ||
| EMS11730-09 | Tris buffered saline, pH 7.4 | 7.4 | 500ml | 2 weeks | AU $92.00 | ||
| EMS11730 | Tris Maleate buffer 0.2M | 6.4-8.0 | 250ml | 2 weeks | AU $69.00 | ||
| EMS11740 | Tris Maleate buffer 0.2M | 6.4-8.0 | 500ml | 2 weeks | AU $92.00 | ||
| EMS11730-20 | Tris-HCL buffer | 7.2-9.0 | 250ml | 2 weeks | AU $69.00 | ||
| EMS11730-25 | Tris-HCL buffer | 7.2-9.0 | 500ml | 2 weeks | AU $92.00 | ||
| EMS11730-12 | Tris-HCL buffer 1M | 6.0-8.0 | 250ml | 2 weeks | AU $69.00 | ||
| EMS11730-15 | Tris-HCL buffer 1M | 6.0-8.0 | 500ml | 2 weeks | AU $92.00 | ||
| EMS25880-01 | Universal Immuno assay buffer 10x | 100ml | 2 weeks | AU $147.00 | |||
| EMS25880-10 | Universal Immuno assay buffer 10x | 1L | 2 weeks | AU $387.00 |