A buffer is a solution that can resist pH change with the addition of an acid or base. It is able to neutralise small amounts of added acid or base, thus maintaining the pH of the solution relatively stable. This is important for processes and or reactions which require specific and stable pH ranges.

Buffers generally consist of a weak acid and its salt or a weak base and its salt. The solutions resist pH change because of a chemical equilibrium between the weak acid HA and its conjugate base A:

HA ⇌ H+ + A
 

Buffers are used with fixatives which require certain properties: 

1. A constant pH during the time of the fixation (The pH of the fixative in electron microscopy is adjusted using the buffer system chosen)

2. Prevention of the extraction of cellular components, or the deposition of precipitates of fixative chemicals. 

A wide range of buffer solutions and powders are available, suitable for most laboratory applications.

CHEM Buffers

 

Code Title Option 1 Option 2 Option 3 Availability* Price
EMS26671-02 Acetate buffer (DG) 6.5-8.0 500ml 0 in stock (?) AU $77.00
EMS11480 Acetate buffer 0.2M (DG) 3.5 - 5.6 225ml 0 in stock (?) AU $86.00
EMS11482 Acetate buffer 0.2M (DG) 3.5 - 5.6 500ml 0 in stock (?) AU $132.00
EMS11482-42 Acetate buffer 0.2M (DG) 4.2 500ml 0 in stock (?) AU $132.00
EMS11482-50 Acetate buffer 0.2M (DG) 5 500ml 0 in stock (?) AU $132.00
EMS11482-56 Acetate buffer 0.2M (DG) 5.6 500ml 0 in stock (?) AU $132.00
EMS11455-78 Borate buffer pH 7.8 500ml 0 in stock (?) AU $92.00
EMS11455-82 Borate buffer pH 8.2 500ml 0 in stock (?) AU $96.00
EMS11455-90 Borate buffer pH 9.0 500ml 0 in stock (?) AU $96.00
EMS26303-03 Borate buffer 0.1M 9 500ml 0 in stock (?) AU $77.00
EMS64142-07 Citrate buffer 6 100ml 0 in stock (?) AU $169.00
EMS64142-08 Citrate buffer 6 1L 0 in stock (?) AU $281.00
EMS11490 Citrate buffer 0.1M 4.6-6.2 225ml 0 in stock (?) AU $56.00
EMS11492 Citrate buffer 0.1M 4.6-6.2 500ml 0 in stock (?) AU $73.00
EMS11492-04 Citrate Buffer 0.2M pH 4.0 1L 0 in stock (?) AU $122.00
EMS11492-06 Citrate Buffer 0.2M pH 6.0 1L 0 in stock (?) AU $124.00
EMS11772-05 D-Phosphate buffer saline formula Formula I, 0.15M 500ml 0 in stock (?) AU $80.00
EMS11772-10 D-Phosphate buffer saline formula Formula I, 0.15M 1L 0 in stock (?) AU $100.00
EMS11773-05 D-Phosphate buffer saline formula Formula II, 0.19M 500ml 0 in stock (?) AU $80.00
EMS11773-10 D-Phosphate buffer saline formula Formula II, 0.19M 1L 0 in stock (?) AU $102.00
EMS11774-05 D-Phosphate buffer saline formula Formula III, 0.15M 500ml 0 in stock (?) AU $89.00
EMS11774-10 D-Phosphate buffer saline formula Formula III, 0.15M 1L 0 in stock (?) AU $109.00
EMS64142-09 EDTA buffer (10X) 8.5 100ml 0 in stock (?) AU $169.00
EMS64142-10 EDTA buffer (10X) 8.5 1L 0 in stock (?) AU $281.00
EMS16530 Glutaraldehyde 10% in solvent (DG) Acetone 10 x 10ml 0 in stock (?) AU $194.00
EMS16531 Glutaraldehyde 10% in solvent (DG) Ethanol 10 x 10ml 0 in stock (?) AU $194.00
EMS16532 Glutaraldehyde 10% in solvent (DG) Methanol 10 x 10ml 0 in stock (?) AU $194.00
AHEP68-1L Hepes buffer solution 6.8 1L 0 in stock (?) AU $168.00
EMS11494 Hepes buffer solution, 0.2M 7.0-8.0 500ml 0 in stock (?) AU $71.00
C018 Hepes buffer, powder 7.0-8.0 25g 0 in stock (?) AU $46.00
C0181 Hepes buffer, powder 7.0-8.0 100g 0 in stock (?) AU $119.00
EMS18041-05 Michel's buffer 7.4 500ml 0 in stock (?) AU $71.00
EMS18041-10 Michel's buffer 7.4 1L 0 in stock (?) AU $97.00
EMS11582-05 Millonig's phosphate buffer, 0.2M 7.4 500ml 0 in stock (?) AU $73.00
EMS11582-10 Millonig's phosphate buffer, 0.2M 7.4 1L 0 in stock (?) AU $102.00
EMS18900 Mops biological buffer 6.5-7.9 100g 0 in stock (?) AU $161.00
EMS11162 PHEM buffer 0.1M 225ml 0 in stock (?) AU $118.00
EMS11163 PHEM buffer 0.1M 500ml 0 in stock (?) AU $193.00
EMS11164 PHEM buffer 0.2M 225ml 0 in stock (?) AU $118.00
EMS11165 PHEM buffer 0.2M 500ml 0 in stock (?) AU $193.00
EMS19340-60 Phosphate buffer 0.1M (EMS) 6 1L 0 in stock (?) AU $116.00
EMS19340-65 Phosphate buffer 0.1M (EMS) 6.5 1L 0 in stock (?) AU $116.00
EMS19340-70 Phosphate buffer 0.1M (EMS) 7 1L 0 in stock (?) AU $116.00
EMS19340-72 Phosphate buffer 0.1M (EMS) 7.2 1L 0 in stock (?) AU $116.00
EMS19342-10 Phosphate buffered saline, 10X (EMS) 7.4 1L 0 in stock (?) AU $100.00
EMS19342-40 Phosphate buffered saline, 10X (EMS) 7.4 3785ml 0 in stock (?) AU $281.00
EMS19344-10 Phosphate buffered saline, 1X (EMS) 7.4 1L 0 in stock (?) AU $84.00
EMS19344-20 Phosphate buffered saline, 1X (EMS) 7.4 3785ml 0 in stock (?) AU $243.00
EMS19343-10 Phosphate buffered saline, 2X (EMS) 7.4 1L 0 in stock (?) AU $94.00
EMS19343-40 Phosphate buffered saline, 2X (EMS) 7.4 3785ml 0 in stock (?) AU $263.00
APBS-1L Phosphate buffered saline, pH 7.4 7.4 1L 0 in stock (?) AU $121.00
EMS11610 Pipes buffer solution 0.3M 5.5-6.0 500ml 0 in stock (?) AU $127.00
C022 Pipes buffer, powder 6.4-7.2 25g 0 in stock (?) AU $109.00
C0221 Pipes buffer, powder 6.4-7.2 100g 0 in stock (?) AU $325.00
EMS62706-12 R-Buffer (DG) for retriever slide stainer 4.5 (C) 250ml 0 in stock (?) AU $172.00
EMS62706-10 R-Buffers for retriever slide stainer 6.0 (A) 250ml 0 in stock (?) AU $172.00
EMS62706-11 R-Buffers for retriever slide stainer 8.0 (B) 250ml 0 in stock (?) AU $172.00
EMS62706-13 R-Buffers for retriever slide stainer 6.0 (U) 250ml 0 in stock (?) AU $172.00
EMS62707-10 R-Buffers for retriever slide stainer 6.0 (Ag) 250ml 0 in stock (?) AU $172.00
EMS62707-11 R-Buffers for retriever slide stainer 8.0 (Bg) 250ml 0 in stock (?) AU $172.00
EMS62707-12 R-Buffers for retriever slide stainer 4.5 (Cg) 250ml 0 in stock (?) AU $172.00
EMS62707-13 R-Buffers for retriever slide stainer 6.0 (Ug) 250ml 0 in stock (?) AU $172.00
EMS62719-10 R-Universal epitope recovery buffer 125ml 0 in stock (?) AU $374.00
EMS62719-20 R-Universal epitope recovery buffer 500ml 0 in stock (?) AU $578.00
EMS15953 Sodium cacodylate buffer 7.4 10 x Vials/10ml 0 in stock (?) AU $100.00
EMS11650 Sodium cacodylate buffer 0.2M 7.4 225ml 0 in stock (?) AU $80.00
EMS11652 Sodium cacodylate buffer 0.2M 7.4 500ml 0 in stock (?) AU $114.00
EMS11653 Sodium cacodylate buffer 0.2M 7.4 1L 0 in stock (?) AU $203.00
EMS11654 Sodium cacodylate buffer 0.4M 7.2 500ml 0 in stock (?) AU $149.00
EMS11655 Sodium cacodylate buffer 0.4M 7.2 1L 0 in stock (?) AU $254.00
C216 Sodium hydrogen phosphate, monobasic 4.1-4.5 500g 0 in stock (?) AU $98.00
EMS11682-05 Sorensen's phosphate buffer 0.15M 7.4 500ml 0 in stock (?) AU $80.00
EMS11682-10 Sorensen's phosphate buffer 0.15M 7.4 1L 0 in stock (?) AU $103.00
EMS11600-05 Sorensen's phosphate buffer 0.2M 7.2 500ml 0 in stock (?) AU $73.00
EMS11600-10 Sorensen's phosphate buffer 0.2M 7.2 1L 0 in stock (?) AU $102.00
EMS11600-40 Sorensen's phosphate buffer 0.2M 7.2 4L 0 in stock (?) AU $306.00
EMS11601-10 Sorensen's phosphate buffer 0.2M 7.4 1L 0 in stock (?) AU $103.00
EMS11601-40 Sorensen's phosphate buffer 0.2M 7.4 4L 0 in stock (?) AU $307.00
ASOR68C-1L Sorensen's phosphate buffer, 2M 6.8 1L 0 in stock (?) AU $74.00
ASOR68C-5L Sorensen's phosphate buffer, 2M 6.8 5L 0 in stock (?) AU $313.00
EMS11730-05 Tris buffer 0.2M 8 250ml 0 in stock (?) AU $66.00
EMS11730-06 Tris buffer 0.2M 8 500ml 0 in stock (?) AU $89.00
EMS11730-08 Tris buffered saline, pH 7.4 7.4 250ml 0 in stock (?) AU $66.00
EMS11730-09 Tris buffered saline, pH 7.4 7.4 500ml 0 in stock (?) AU $89.00
EMS11730 Tris Maleate buffer 0.2M 6.4-8.0 250ml 0 in stock (?) AU $66.00
EMS11740 Tris Maleate buffer 0.2M 6.4-8.0 500ml 0 in stock (?) AU $89.00
EMS11730-20 Tris-HCL buffer 7.2-9.0 250ml 0 in stock (?) AU $66.00
EMS11730-25 Tris-HCL buffer 7.2-9.0 500ml 0 in stock (?) AU $89.00
EMS11730-12 Tris-HCL buffer 1M 6.0-8.0 250ml 0 in stock (?) AU $66.00
EMS11730-15 Tris-HCL buffer 1M 6.0-8.0 500ml 0 in stock (?) AU $89.00
EMS25880-01 Universal Immuno assay buffer 10x 100ml 0 in stock (?) AU $141.00
EMS25880-10 Universal Immuno assay buffer 10x 1L 0 in stock (?) AU $365.00