Aurion BSA-c (10%) (acetylated BSA)

SKU: JA900-099-10   |   Availability ⓘ

Aurion BSA-c (10%) (acetylated BSA)

SKU: JA900-099-10   |   Availability ⓘ
Pack Size
AU $355.00 Regular price

Taxes and shipping applied at checkout.

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Aurion has developed BSA-c™, a unique incubation buffer additive with an unparalleled ability to effectively prevent charge based background. BSA-c™ is prepared by acetylation of bovine serum albumin (BSA). Polycationic sites in the specimen interact readily with negatively charged acetylated BSA molecules. This significantly reduces the risk that such sites might bind negatively-charged immunoreagents and immunogold conjugates and thus reduces the risk of background.

Store refrigerated 4-8 deg C

Product Description 

AURION BSA-c™ concentrated solution contains acetylated bovine serum albumin as the functional constituent. By acetylation of amines on basic amino acids these groups are no longer as easily protonated and the isoelectric point of such molecules is lowered and hydrophobicity is increased. BSA-c™ is a 10% solution of acetylated BSA at slightly alkaline pH with Kathon CG as preservative. The bovine serum albumin that Aurion uses to prepare BSA-c™ is obtained from healthy livestock. The charge dependent background inhibition capacity of the BSA-c™ in each lot is determined using a dot-spot test system with polycationic compounds. Two package volumes are available which yield 1.5 - 3 and 5 -10 litres of incubation buffer respectively

Application

Procedures to eliminate background comprise three main steps: 

• To suppress residual aldehyde activity
• To saturate multipoint hydrophobic moieties and positive charges with high molecular weight compounds such as those present in the AURION Blocking Solutions
• To reduce aspecific binding of immunoreagents caused by hydrophilic interaction with competing molecules in the incubation and washing solution. 

AURION BSA-c™ is a particularly effective reagent for this purpose.

AURION BSA-c™ is a buffer additive that helps prevent immunodetection reagents (i.e. primary antibodies and secondary reagents) from binding nonspecifically to charged moieties within the specimen. Thus, it suppresses background competitively with little or no effect on the specific reaction. Its successful application is not limited to immunogold detections but it is equally efficient in fluorescent and enzyme-based detection systems. AURION BSA-c™ concentrations as low as 0.01-0.1% inhibit binding of gold conjugate to polycationic poly-l-lysine coated grids almost completely (>99%). The surface properties of the specimen can be simplified by division into four compartments: - negatively charged (polyanions, proteins, especially after aldehyde fixation, lipids);
0 neutral;
+ positively charged (histone proteins, polycations) and;
H hydrophobic (lipids, fat droplets, resins). 

After an appropriate blocking step these areas are covered with blocking compounds. In low ionic strength media negatively charged antibodies and gold conjugates are repulsed by negatively charged specimen areas which frequently may contain the antigens to be detected. Background does not likely occur in such areas. The positively charged areas attract antibodies and gold conjugates potentially leading to background. In a moderate ionic strength incubation solution, repulsion and attraction are diminished due to the presence of ions. The negatively charged BSA-c™ competes with the negatively charged antibodies and gold reagents for non-specific binding to the positively charged specimen compounds, thus reducing background to the greatest possible extent without interfering with antigen detection.

Recommended Incubation Solution: 

PBS, (10 mM Phosphate buffer, 150 mM NaCl)
0.1-0.2 % AURION BSA-c™ 15 mM NaN3 pH 7.4
After addition of the BSA-c™ concentrate the pH should be checked and adjusted to 7.4 if necessary.

This Incubation Solution is used throughout the whole set of reactions and washing steps (i.e. from immediately after the blocking step, through the primary antibody incubations, in between washing steps, secondary incubation and the final washing steps).

Storage 
AURION BSA-c™ concentrate has a guaranteed shelf life of 18 months from the date of quality control analysis.
The products should be stored at 4-8°C. Freezing is not recommended.

Aurion BSA-c (10%) (acetylated BSA)
Updated: 24-07-2024
Code Pack Size Availability* Price (ex GST)
JA900-099-10 3x10ml 0 in stock (?) AU $355.00
EMS25558 10 x 10ml 0 in stock (?) AU $536.00

REFUNDS AND RETURNS

Please ask prior to returning any goods.

Defective products should be returned to us for replacement or credit. It is best to contact support with as much detail as possible, including pictures, so we can quickly resolve the issue. ProSciTech Pty Ltd complies with the ACCC guidelines, available here: https://www.accc.gov.au/consumers/consumer-rights-guarantees/repair-replace-refund 

If you decide the item purchased does not suit your requirements, you may contact us to see if a return is possible. Some items may not be refunded, so please ask before purchase if you think a return may be necessary. The greater of $15 or 15% re-stocking charge  will be levied on materials returned due to an error on the part of the customer. We try to keep this charge minimal, however returns do have a cost to us.

SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200. 

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

ProSciTech will choose the courier for your order with consideration for the destination, value of parcel, weight and perishability of contents. It is our concern that our shipments reach you, our customer, rapidly and in good condition. Please contact us if you have any issues with a shipment and we will do our best to resolve it. Please keep in mind however, that our legal responsibility ends when goods are passed to the carrier.

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $20
Express shipping $30

Delivery times:

Check your email for tracking details provided at time of shipping for updates of your parcel. We cannot guarantee delivery times, as this is outside of our control. The following are the delivery estimates as given by our couriers. 

Regular shipping
Queensland   1-3 business days 
Sydney   2-5 business days 

Melbourne, Adelaide, Hobart or Canberra 

 3-10 business days 

WA, NT and non-metro areas

 4-10 business days 
Express shipping
Australia wide   1-2 business days

Urgent Orders:

Please request "Express" if you would like us to upgrade your shipment to express delivery. Providing your order fits in a satchel and/or is under 1kg in weight, we will ship your order either DHL, TNT or Express Post. Larger or heavy orders need a quotation for additional freight costs.

Dangerous goods:

See the table on the page for DG shipment costs by road. Please specify when ordering if you require air service (this can be prohibitively expensive). Please request a quotation. We will charge at cost.
 

Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping. 

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

GUARANTEE

All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods. 

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted) 

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products. 

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Trouble Shooting

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