Aurion gold nanoparticles, carboxyl functionalised
Aurion gold nanoparticles, carboxyl functionalised
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Aurion Gold Nanoparticles (Carboxyl Functionalised) are polyethylene glycol-carboxyl stabilised gold nanoparticle solutions based on defined particle sizes of 6, 10, 15 and 25nm. They are prepared using in-house developed production protocols giving the product a narrow size distribution, long term stability and optimal conjugation properties. Biomolecules having primary amines available can be covalently conjugated using EDC/sulfo-NHS chemistry.
Features
- Carboxyl (COOH) functionalised
- Particle sizes 6, 10, 15 and 25nm
- Bind to primary amino amines in target molecule
- Conjugation using EDC/sulfo-NHS chemistry
- Recommended for conjugation of small ligands
- Detailed conjugation protocol
- Ready and easy to use, long term stability
Biomolecules that are too small to be conjugated to Gold Nanoparticles via adsorption can be covalently linked to Carboxyl-functionalised Gold Nanoparticles. Primary amines are present in e.g. the N-terminal side of peptides and in the side group of the amino acid lysine. The conjugation relies on well known and proven EDC/sulfo-NHS chemistry. EDC (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride) is a water soluble carbodiimide which transforms the carboxyl groups on the gold to an active ester in the presence of sulfo-NHS (N-Hydroxy-sulfosucciimide, sodium salt). These sulfo-NHS esters are relatively stable in acidic environment and couple rapidly to the amine(s) in the target molecules. Aurion Gold Nanoparticles (Carboxyl Functionalised) are prepared according to a unique protocol, warranting narrow size distribution, long term stability and optimal conjugation properties. They are available in size ranges 6, 10, 15 and 25nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6 and 25nm particle size sols is less than 12%, whereas the 10 and 15nm size sols show less then 10% variation. Actual lot specifications (size, variation and expiry date) are indicated on the accompanying package insert. Aurion Gold Nanoparticles (Carboxyl Functionalised) are supplied in 10mM MES buffer, pH 5.0. Package size: 4x5 ml of high quality Carboxyl-functionalised Gold Nanoparticles at an OD520nm = 1.0.
Aurion Gold Nanoparticles (Carboxyl Functionalised) have a guaranteed shelf life of 12 months from the date of quality control analysis.
These are ordered fresh.
Store refrigerated 4-8°C
Images are illustrative only. Please check product details before ordering.
