Aurion R-Gent silver enhancement kit

SKU: JA500-033   |   Availability ⓘ

Aurion R-Gent silver enhancement kit

SKU: JA500-033   |   Availability ⓘ
Style
Pack Size
AU $445.00 Regular price

Taxes and shipping applied at checkout.

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AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface.

AURION R-Gent SE-EM is available in a kit containing the following:

  • 30 ml of ready-to-use ENHANCER,
  • 3 ml of concentrated INITIATOR and
  • 30 ml of ACTIVATOR.
  • a 3 ml empty dropping bottle, labelled "DEVELOPER".

For many other applications, the detection in electron microscopy of ultra small gold particles requires a process of particle enhancement. For this purpose, Danscher 's method is very much the standard, and attempts to improve the features of Danscher 's system met with only minimal success over a long period of time.  

The aim of the development of AURION R-Gent SE-EM was to establish a system with greater efficiency and homogeneity, at least comparable to the Danscher method, however with reduced acidity and sensitivity to light. The reagents should also have low viscosity for suitability for pre-embedding immunogold labelling.

Introduction

The silver enhancement response is a gold particle-catalyzed reduction in which silver ions are converted to metallic silver with the electron source being a photographic forming compound. In addition, a "protective colloid" is incorporated to the enhancement solution in many applications to limit the diffusion of reagents to the surface of the gold particles, thus providing a way of controlling particle growth.

Product Description

AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected and is compatible with heavy metal counterstaining. The reagent has extremely delayed auto-nucleation and can be used under standard laboratory light conditions. It also has low viscosity, which is especially advantageous for pre-embedding immunogold applications. The enhancement mixture has a pH of 8.1-8.2.

AURION R-Gent SE-EM has been tested ntensively for the enhancement of AURION Ultra Small Immunogold reagents.

The INITIATOR is a concentrated solution which must be diluted and activated before use, using the ACTIVATOR. This resulting mixture is the DEVELOPER.

The now ready-to-use DEVELOPER has a shelf life of one month. It is suggested to prepare fresh DEVELOPER at least every month.

The undiluted solutions have a shelf life of 10 months when stored at 4°C. INITIATOR can be stored at -20°C for prolonged shelf life.

For enhancement one drop of DEVELOPER is mixed with 20 drops of ENHANCER. The typical enhancement time is between 20 and 40 minutes. Specimens may be contrasted according to standard procedures.

The kit with 30 ml of ENHANCER accomodates up to 1000 EM grid specimens. The kit with 90 ml of ENHANCER is suitable for pre-embedding immunogold labeling; the number of specimens it accomodates depends on the volume of reagent used per specimen.

For each lot, the specific enhancement activity and level of autonucleation are monitored by spectrophotometric techniques.

Storage:

The AURION R-Gent SE-EM components are stored at 4°C and allowed to reach room temperature before use. SE-EM INITIATOR may be stored at -20°C for prolonged shelf life

Aurion R-Gent silver enhancement kit
Updated: 17-04-2024
Code Style Pack Size Availability* Price (ex GST)
JA500-033 for EM 30ml 0 in stock (?) AU $445.00
EMS25521-90 for EM 90ml 0 in stock (?) AU $801.00
JA500-011 for LM 2x30ml 0 in stock (?) AU $311.00
JA500-022 for LM 2x250ml 0 in stock (?) AU $430.00

REFUNDS AND RETURNS

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If you decide the item purchased does not suit your requirements, you may contact us to see if a return is possible. Some items may not be refunded, so please ask before purchase if you think a return may be necessary. The greater of $15 or 15% re-stocking charge  will be levied on materials returned due to an error on the part of the customer. We try to keep this charge minimal, however returns do have a cost to us.

SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200. 

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

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Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $20
Express shipping $30

Delivery times:

Check your email for tracking details provided at time of shipping for updates of your parcel. We cannot guarantee delivery times, as this is outside of our control. The following are the delivery estimates as given by our couriers. 

Regular shipping
Queensland   1-3 business days 
Sydney   2-5 business days 

Melbourne, Adelaide, Hobart or Canberra 

 3-10 business days 

WA, NT and non-metro areas

 4-10 business days 
Express shipping
Australia wide   1-2 business days

Urgent Orders:

Please request "Express" if you would like us to upgrade your shipment to express delivery. Providing your order fits in a satchel and/or is under 1kg in weight, we will ship your order either DHL, TNT or Express Post. Larger or heavy orders need a quotation for additional freight costs.

Dangerous goods:

See the table on the page for DG shipment costs by road. Please specify when ordering if you require air service (this can be prohibitively expensive). Please request a quotation. We will charge at cost.
 

Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping. 

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

GUARANTEE

All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods. 

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted) 

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products. 

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