PP3006 CoolLok cryo-SEM transfer system

SKU: QUO24429   |   Availability ⓘ

PP3006 CoolLok cryo-SEM transfer system

SKU: QUO24429   |   Availability ⓘ
Type
Pack Size
P.O.R.


The CoolLok offers rapid transfer and cryo temperature observation of specimens for SEM, FIB/ SEM, beam line or other vacuum systems. Applications include thermal protection of beam sensitive specimens and low temperature observation of materials such as plastics, polymers, low-K dielectrics and hard-soft mixtures. The system can also be used for inert gas transfer of ambient temperature specimens from a glove box.

This does not replace the PP3010T, which is a full cryo preparation system (with cold fracturing, sputtering etc.), but can be useful for observing a range of solid, non-water based, materials under cryo conditions.

Walk through the Cryo-SEM Preparation System Laboratory

Features:

  • Rapid specimen exchange
  • Temperature range down to -190°C
  • Off-column cooling with all-day runtime between fills
  • Independent cooling of cold stage and cold trap
  • Vacuum or inert gas (glove box) transfer
  • Rapid specimen freezing option QUO24425
  • Cryo workflow options
  • Extended warranty option
  • Various stubs and shuttles available separately.

System components

Vacuum airlock and cooling gas feedthrough
Mounted onto a suitable vacuum chamber port, the CoolLok consists of a loading chamber body with built-in controls for pumping, venting and transfer. A custom-designed interface flange to the vacuum chamber and connections and fittings to the pumping system are included (see: Pumping below). The interface has cold nitrogen gas feeds to and from the microscope cold stage and cold trap.

Specimen holders and transfer device
The compact vacuum transfer device has a bayonet fitting to a dovetail-profile specimen holder (shuttle). Standard shuttles are included, but optional holders allow a range specimen types to be handled.

Cold stage and cold trap
A highly stable and thermally isolated nitrogen gas-cooled cold stage attaches to the SEM stage. The location and shape of the cold trap is tailored to suit the internal geometry of the microscope. Both cold stage and cold trap are capable of reaching temperatures down to -190°C with a stability of <0.5°C. For easy specimen exchange an interlocked LED chamber light is fitted.
The cold stage connects to the SEM stage using an adaptor and has a dovetail fitting to accept a cryo specimen holder. When not in use the cold stage is uncoupled and stored within the chamber with the gas and electrical fittings connected.

Cooling dewar, trolley and controller
The cold stage and cold trap are cooled by a floor-mounted, vacuum isolated 21 litre dewar and heat exchanger assembly, which at normal operating temperatures can run for up to 24 hours between fills. The gas lines between the dewar and the SEM interface are vacuum isolated for maximum thermal efficiency.
The cooling dewar is located on a floor-mounted trolley which also houses the temperature monitor/controller for the cold stage and temperature monitor for cold trap, plus nitrogen gas flow controllers.

Rapid freezing station (QUO24429)
With the standard CoolLok, specimen freezing is by contact with the SEM cold stage following transfer and therefore freezing rates are relatively slowly. This is suitable for hard, non-hydrated specimens, but for liquid-based material rapid freezing is essential to reduce the detrimental effects of ice crystal growth and to allow through-vacuum transfer onto the cold stage.
For these applications the optional QUO24429 nitrogen slush freezing station is required. However, for many applications (especially life sciences) cold fracturing and sputter coating are essential process steps and require the advanced capabilities of the QUOPP3010T - a full cryo-SEM preparation system.

Using the CoolLok
The specimen is mounted on a suitable holder (shuttle) and the transfer device fitted onto the airlock and the dead space evacuated to a set vacuum level. The gate valve is opened and the specimen guided onto the SEM stage.
For transfers from other vacuum systems, or a glove box, additional interface flanges are available on request. Vacuum transfers can be made from the optional QUO24429 trolley-mounted nitrogen slush freezing station, if fitted.

Pumping
The CoolLok requires either a rotary pump or an oil-free turbo pumping station (see Ordering Information).

Technical Data

Specifications:

Model
PP3004 PP3005 PP3006
Temperature Ambient RT to -190°C
Cooling runtime N/A Up to 24hrs
Dewar capacity N/A 30L
Cool down time to -190°C * N/A <15 minutes
*Under standard laboratory conditions
Rapid freezing (slushy LN2) N/A Optional (QUO24429)
Dewar trolley footprint N/A 50 x 50cm
Airlock weight 2.5kg
Pumping requirements Turbo Pump Rotary Pump Turbo Pump
Nitrogen gas Venting and valve operation Venting and cooling
Power requirements (excluding pump) 300W
Maximum specimen size Flat specimens up to 23 x 26mm. For taller specimens the maximum height will reduce from a mid-point of 9mm.

PP3004-6 product brochure


Ordering Information

QUOPP3006 Includes: Airlock assembly. Pump and vent control buttons, gate valve and fittings to the pumping system (see: Pumping below). Custom-designed interface flange to the microscope vacuum chamber. Cooling system. Nitrogen gas cooled stage with heater and sensor and cold trap with temperature sensor. Precise temperature control with a range down to 190°C, 21L liquid nitrogen dewar with trolley, heat exchanger and LED chamber light. QUO12340 specimen transfer device.
Specimen holders:
3 x QUOAL200077B specimen shuttle (to hold 10mm cryo stubs)
QUO12434 blank specimen shuttle
QUO20720 specimen shuttle with holding clips
QUOE7402 blank 10mm stubs (pack/10)
5 x QUOE7449-7 multi-purpose specimen stubs.
Note: other optional holders are available. Specimen mounting compounds (colloidal graphite and Tissue-Tek), interlock cable and pump fittings.
Pumping - The PP3006 CoolLok requires either a rotary pump or an oil-free, high vacuum turbomolecular pumping station (recommended).
QUO13034 Pfeiffer DUO 6 two-stage rotary vacuum pump, 240V
Options and accessories:
QUO24429 Rapid cooling station consisting of a floor-mounted trolley, liquid nitrogen freezing chamber mounted into the work surface which interfaces to the cryo transfer device, connections to vacuum pump (order separately).
QUOPP7450/60L Pressurised (60L) liquid nitrogen dewar. Boil-off nitrogen gas is used for cooling stage and cold trap.
QUO13296 Sircal in-line gas dryer. Helps to reduce the water content of the nitrogen gas supply, if an external gas source is used.


Updated: 07-10-2024
Code Type Pack Size Availability* Price (ex GST)
QUO24429 Rapid cooling station Each 10 weeks P.O.R.
QUOPP3006 PP3006 CoolLok Each 10 weeks P.O.R.
QUOPP7450/60L Pressurised LN2 dewar Each 10 weeks P.O.R.

* Availability Explanation

X weeks – the estimated lead time, based on the average time taken previously to fill orders from this particular supplier. Lead times are from when we receive your order until when we fill your order. For delivery times, please see shipping information below.

In Stock – Plenty of stock on the shelf, ready to ship.

No ETA – our supplier has advised they cannot provide an accurate ETA. Generally there is some issue with supply, and it may be more than 6 months.

REFUNDS AND RETURNS

Please ask prior to returning any goods.

Defective products should be returned to us for replacement or credit. It is best to contact support with as much detail as possible, including pictures, so we can quickly resolve the issue. ProSciTech Pty Ltd complies with the ACCC guidelines, available here: https://www.accc.gov.au/consumers/consumer-rights-guarantees/repair-replace-refund 

If you decide the item purchased does not suit your requirements, you may contact us to see if a return is possible. Some items may not be refunded, so please ask before purchase if you think a return may be necessary. The greater of $15 or 15% re-stocking charge  will be levied on materials returned due to an error on the part of the customer. We try to keep this charge minimal, however returns do have a cost to us.

SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200. 

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

ProSciTech will choose the courier for your order with consideration for the destination, value of parcel, weight and perishability of contents. It is our concern that our shipments reach you, our customer, rapidly and in good condition. Please contact us if you have any issues with a shipment and we will do our best to resolve it. Please keep in mind however, that our legal responsibility ends when goods are passed to the carrier.

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $20
Express shipping $30

Delivery times:

Check your email for tracking details provided at time of shipping for updates of your parcel. We cannot guarantee delivery times, as this is outside of our control. The following are the delivery estimates as given by our couriers. 

Regular shipping
Queensland   1-3 business days 
Sydney   2-5 business days 

Melbourne, Adelaide, Hobart or Canberra 

 3-10 business days 

WA, NT and non-metro areas

 4-10 business days 
Express shipping
Australia wide   1-2 business days

Urgent Orders:

Please request "Express" if you would like us to upgrade your shipment to express delivery. Providing your order fits in a satchel and/or is under 1kg in weight, we will ship your order either DHL, TNT or Express Post. Larger or heavy orders need a quotation for additional freight costs.

Dangerous goods:

See the table on the page for DG shipment costs by road. Please specify when ordering if you require air service (this can be prohibitively expensive). Please request a quotation. We will charge at cost.
 

Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping. 

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

GUARANTEE

All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods. 

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted) 

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products. 

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