Thermanox coverslips, circular
Thermanox coverslips, circular
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Unbreakable coverslips, these have been treated for optimum cell attachment and growth. Useful in most tissue culture applications in place of glass coverslips. Suitable for scintillation counting. Thermanox coverslips have the same thickness as No. 2 (0.2mm) glass coverslips.
Thermanox coverslips are culture treated on one side for enhanced cell attachment and growth. The treated side is packaged facing up toward the label.
Features
- Material: flexible, transparent polymer in the polyolefin family
- Low gas permeability
- Solvent resistance: may be used with amyl acetate, glutaraldehyde or propylene oxide for EM preparations. Also resistant to acetone, alcohol, xylene and other commonly used solvents.
- Chemical resistance: alcohols, aldehydes, hydrocarbons, dilute acids (<10%) and dilute alkalis (<2%).
- Limited resistance : chlorinated hydrocarbons
- No resistance: concentrated acids or concentrated bases
- Easy cut: can be sectioned using a microtome and can be easily cut with scissors.
- Promotes cell attachment : Cell attachment and growth are comparable to polystyrene and glass
- Temperature resistant : -70?C to +150?C
- Auto fluorescent : range 380 to 545 nm
- Supplied sterile - Can be autoclaved at 20 minutes steam, 20 minutes dry at 120?C
Technical Tip
Permanox Plastic; Non Fluorescent: Permanox plastic is nonfluorescent and may be used with most fluorescent labels. Permanox has better chemical resistance to fixation and staining solvents than polystyrene. An aqueous based mounting medium must be used when cover slipping to prevent warping.
Coverslip treatment test: A simple method to determine which side of the coverslip is treated is the "droplet" test. A drop of water or culture medium will spread on the hydrophilic, treated side. - If the droplet forms a bead, then the side of the coverslip is not treated. - If the droplet spreads evenly over the coverslip, the side is treated.
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