Diamond knife, Cryo Dry, 25 deg.
SKU: UCD25-30 | Availability ⓘ
Diamond knife, Cryo Dry, 25 deg.
SKU: UCD25-30 | Availability ⓘ
DiATOME Cryo diamond knives may be used for all low temperature situations, such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.
There are numerous advantages to our Cryo diamond knives compared with glass knives:
There are numerous advantages to our Cryo diamond knives compared with glass knives:
- They produce thinner sections
- Ultrathin and semithin sections with the same knife
- Easy to produce long ribbons of sections
Less compression and greater uniformity of sections
- Time saving. No knife manufacturing, no knife changing, hence no waiting for temperature equilibration.
- Diamond is the best temperature conductor—better than gold. No waiting for temperature equilibration
Cryo 25°
The Cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.
Cryo 35° and Cryo 45°
The 45° knife is well suited for routine cryo-sectioning.
The 35° knife allows sectioning with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).
The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.
The seal is made of a cold-resistant epoxy resin.
The Cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.
Cryo 35° and Cryo 45°
The 45° knife is well suited for routine cryo-sectioning.
The 35° knife allows sectioning with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).
The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.
The seal is made of a cold-resistant epoxy resin.
** Resharpened DiATOME knives undergo the same stringent optical checking and sectioning tests as new knives. If you are wanting a re-sharpened service, you will post your old knife to us and we will send away to our supplier.
The lead time for re-sharpening knives is ~ 10-12 weeks from the receipt of your knife.