NIGHTSEA Leica EZ4 microscope fluorescence viewing systems, pulse base option
NIGHTSEA Leica EZ4 microscope fluorescence viewing systems, pulse base option
The Leica EZ4 Mounting Adapter enables you to use the NIGHTSEA Stereo Microscope Fluorescence System with the Leica EZ4 series of stereo microscopes, with or without integrated camera. Easily attach the barrier filter magnetically and hold the filter shield in place with a thumbscrew. The adapter is available for purchase separately to quickly move between Leica and other stereo microscopes.
Full system with Leica EZ4 Adapter & one illumination colour consisting of:
- Lamp Base with Power Supply (standard, dim and pulse available)
- Light Head-Royal Blue, Cyan, Green, Violet or Ultraviolet
- Leica EZ4 Mounting adapter
- Barrier Filter
- Viewing Shield
Modular - Installs in seconds and just clicks into place. Interchangeable excitation/emission combinations that can move from microscope to microscope without modification needed to your microscope.
Economical - Stretch your lab budget with inexpensive enough for classroom use or buy just what you need now (up to 6 different wavelength sets) and add more as your needs expand.
Applications:
- Quick screening of your fluorescent genotypes - Drosophila, zebrafish, C. elegans
- Genotype sorting
- Fluorescence-aided dissection, injection, or micromanipulation
- Pre-screening sample preps for confocal or other high-resolution imaging
- Freeing up your research-grade fluorescence microscopes for more demanding work
- Coating and failure analysis, circuit board work, defect location, food safety, paper analysis, and more
The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light+filter combinations.
An oversize adapter and microscope mounting adapter (up to 67mm) for stereo microscopes are also available separately.
Once you are set up for one excitation/emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and colour coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.
Wavelength Sets
For more in-depth information on excitations, see this technical data sheet
Specifications
| Designation | Excitation | Emission | Fluorophores |
| UV - Ultra Violet | 360-380nm | 415nm LP | DAPI |
| VI - Violet | 400-415nm | 450nm LP | CFP |
| RB - Royal Blue | 440-460nm | 500nm LP | GFP, eGFP, fluorescein |
| RB-GO - Green Only | 440-460nm | 500-560nm BP | GFP, eGFP, fluorescein |
| CY - Cyan | 490-515nm | 550nm LP | YFP, Venus, Lucifer Yellow |
| GR - Green | 510-540nm | 600nm LP | DsRed, dTomato |
Green-Only Barrier Filter
The Green-Only (GO) barrier filter isolates the green part of the spectrum and is for use with the royal blue excitation source. While our other barrier filters are longpass filters this filter is a bandpass, transmitting from approximately 500 to 560nm. The longpass filter has served well for most users who need to visualise green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.
 GFP-tagged Drosophila larva. Photo: NIGHTSEA/Charles Mazel |
 NIGHTSEA adapter |
 NIGHTSEA microscope adapter |
Lamp Base Light Control Options
The SFA lamp base is available in three versions: Standard, DIM, and PULSE. Only one control option is available per base. The DIM and PULSE options can be purchased as a part of a new system, or are available as retrofit to an existing base, or in a stand-alone lamp base purchased a la carte.
NIGHTSEA Standard Base - Provides a steady light when turned on, simple OFF/ON operation.
NIGHTSEA DIM Base - BNC connector that accepts a voltage signal for combined switch and dimmer control.
NIGHTSEA PULSE Base - BNC connector on the rear of the base that features an OFF/ON/DIM switch to change the intensity of the light.
Base Specifications:
- Voltage input: 2.8-6.0 VDC for ON, <0.6VDC OFF
- Maximum operation frequency: 10kHz
- BNC cable not included
Images are illustrative only. Please check product details before ordering.
