Name: NIGHTSEA stereo microscope fluorescence viewing systems, pulse base option
Brand: NIGHTSEA
SKU: OSFA-CY-PULSE
Price: 2672.0 AUD
Availability: InStock

Price P.O.R.

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The NIGHTSEA Stereo microscope fluorescence adapter adapts just about any stereo microscope (dissecting microscope) for fluorescence with no modification to the microscope itself. The modular design lets you easily switch between several different excitation/emission combinations to work with a variety of fluorescent proteins and other fluorophores. There are now six different excitation/emission combinations available, plus white light.

Full system with one illumination colour consisting of:

PULSE Base - BNC connector on the rear of the base that accepts a user-supplied voltage input to turn the light on and off and international plug set
Light Head - Ultraviolet, violet, royal blue, cyan, or green
Microscope mounting adapter
Barrier filter
Viewing shield
Padded travel case

Modular - Installs in seconds and just clicks into place. Interchangeable excitation/emission combinations that can move from microscope to microscope without modification needed to your microscope.

Economical - Stretch your lab budget with inexpensive enough for classroom use or buy just what you need now (up to 6 different wavelength sets) and add more as your needs expand.

Applications:

Quick screening of your fluorescent genotypes - Drosophila, zebrafish, C. elegans
Genotype sorting
Fluorescence-aided dissection, injection, or micromanipulation
Pre-screening sample preps for confocal or other high-resolution imaging
Freeing up your research-grade fluorescence microscopes for more demanding work
Coating and failure analysis, circuit board work, defect location, food safety, paper analysis, and more

The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light+filter combinations.

The microscope mounting adapter fits up to 67mm to work with the majority of stereo microscopes. An oversize adapter and an adapter for the Leica EZ4 series are also available separately.

Once you are set up for one excitation/emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and colour coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.

Wavelength Sets
For more in-depth information on excitations, see this technical data sheet

Specifications

Designation	Excitation	Emission	Fluorophores
UV - Ultra Violet	360-380nm	415nm LP	DAPI
VI - Violet	400-415nm	450nm LP	CFP
RB - Royal Blue	440-460nm	500nm LP	GFP, eGFP, fluorescein
RB-GO - Green Only	440-460nm	500-560nm BP	GFP, eGFP, fluorescein
CY - Cyan	490-515nm	550nm LP	YFP, Venus, Lucifer Yellow
GR - Green	510-540nm	600nm LP	DsRed, dTomato

Green-Only Barrier Filter

The Green-Only (GO) barrier filter isolates the green part of the spectrum and is for use with the royal blue excitation source. While our other barrier filters are longpass filters this filter is a bandpass, transmitting from approximately 500 to 560nm. The longpass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.

GFP-tagged Drosophila larva. Photo: NIGHTSEA/Charles Mazel

NIGHTSEA adapter

NIGHTSEA microscope adapter

Tech Tip: Will the Stereo Microscope Fluorescence Adapter Fit my Microscope?

Lamp Base Light Control Options

The SFA lamp base is available in three versions: Standard, DIM, and PULSE. Only one control option is available per base. The DIM and PULSE options can be purchased as a part of a new system, or are available as retrofit to an existing base, or in a stand-alone lamp base purchased a la carte.

NIGHTSEA Standard Base - Provides a steady light when turned on, simple OFF/ON operation.

NIGHTSEA DIM Base - BNC connector that accepts a voltage signal for combined switch and dimmer control.

NIGHTSEA PULSE Base - BNC connector on the rear of the base that features an OFF/ON/DIM switch to change the intensity of the light.

Base Specifications:

Voltage input: 2.8-6.0 VDC for ON, <0.6VDC OFF
Maximum operation frequency: 10kHz
BNC cable not included

NIGHTSEA stereo microscope fluorescence viewing systems, pulse base option

Images are illustrative only. Please check product details before ordering.

Updated: 24-11-2024

Code	Colour	Lamp Base	Pack Size	Availability*	Price (ex GST)
OSFA-CY-PULSE	Cyan	Pulse	Each	0 in stock (?)	P.O.R.
OSFA-GR-PULSE	Green	Pulse	Each	0 in stock (?)	P.O.R.
OSFA-RB-PULSE	Royal blue	Pulse	Each	0 in stock (?)	P.O.R.
OSFA-RB-GO-PULSE	Royal blue, green-only	Pulse	Each	0 in stock (?)	P.O.R.
OSFA-UV-PULSE	Ultra violet	Pulse	Each	0 in stock (?)	P.O.R.
OSFA-VI-PULSE	Violet	Pulse	Each	0 in stock (?)	P.O.R.

* Ordering Schedule

This item is available for back-order where 0 stock is listed.
We order from this manufacturer weekly, with an estimated lead time of 4 weeks.

This is the estimated lead time, based on the average time taken previously to fill orders from this particular supplier.
Lead times are from when we receive your order until when we fill your order. For delivery times, please see shipping information below.

REFUNDS AND RETURNS

Please ask prior to returning any goods.

Defective products should be returned to us for replacement or credit. It is best to contact support with as much detail as possible, including pictures, so we can quickly resolve the issue. ProSciTech Pty Ltd complies with the ACCC guidelines, available here: https://www.accc.gov.au/consumers/consumer-rights-guarantees/repair-replace-refund

If you decide the item purchased does not suit your requirements, you may contact us to see if a return is possible. Some items may not be refunded, so please ask before purchase if you think a return may be necessary. The greater of $15 or 15% re-stocking charge will be levied on materials returned due to an error on the part of the customer. We try to keep this charge minimal, however returns do have a cost to us.

SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200.

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

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Order Value	Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded).	Free
Less than $200	$20
Express shipping	$30

Delivery times:

Check your email for tracking details provided at time of shipping for updates of your parcel. We cannot guarantee delivery times, as this is outside of our control. The following are the delivery estimates as given by our couriers.

Regular shipping
Queensland	1-3 business days
Sydney	2-5 business days
Melbourne, Adelaide, Hobart or Canberra	3-10 business days
WA, NT and non-metro areas	4-10 business days
Express shipping
Australia wide	1-2 business days

Urgent Orders:

Please request "Express" if you would like us to upgrade your shipment to express delivery. Providing your order fits in a satchel and/or is under 1kg in weight, we will ship your order either DHL, TNT or Express Post. Larger or heavy orders need a quotation for additional freight costs.

Dangerous goods:

See the table on the page for DG shipment costs by road. Please specify when ordering if you require air service (this can be prohibitively expensive). Please request a quotation. We will charge at cost.

Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value	Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded).	Free
Less than $200	$26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping.

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

GUARANTEE

All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods.

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted)

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products.

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