Aurion Enhancement conditioning solution

SKU: EMS25830   |   Availability ⓘ

Aurion Enhancement conditioning solution

SKU: EMS25830   |   Availability ⓘ
Pack Size
AU $166.00 Regular price

Taxes and shipping applied at checkout.

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Aurion's Enhancement Conditioning Solution (ECS) is a concentrate that requires a 10x dilution with distilled grade water before use. It helps optimise enhancement by conditioning specimens labeled with (ultra small) gold conjugates before the silver enhancement procedure using Aurion R-Gent SEEM. Aurion ECS is a prerequisite to condition enhanced specimens for a second incubation series in double labeling experiments (Yi et al, 2001, Journal of Histochemistry and Cytochemistry 49(3): 279-283). 

Introduction 

The silver enhancement reaction is a gold particle-catalyzed reduction in which silver ions are reduced to metallic silver with a photographic developing compound as the electron source. In addition in many applications a "protective colloid" is added to the enhancement solution to limit diffusion of reagents to the gold particle surface, thus providing a means for controlling particle growth. 

Product Description

Incubation buffers suited for immuno gold labeling commonly contain ions such as phosphate and chloride which are not compatible with silver ions causing precipitation of insoluble silver salts. These ions therefore need to be thoroughly removed before enhancement. Whereas pure water is suited to fulfil this requirement in the purely chemical sense, it is rarely the best way when working with delicate specimens potentially affecting ultrastructural details vulnerable to drastic changes in ionic concentration. Using water it is also necessary to carefully blot the specimens and remove the water without drying before applying Aurion R-Gent SE-EM. Failing to do so will result in dilution and diminished activity of the silver enhancement reagents, especially when working with small volumes.

Aurion developed Aurion ECS to negotiate optimum conditions for enhancement while at the same time avoiding the risks posed by water. Its ionic strength is similar to immuno incubation buffers and its make up is compatible both with these buffers as well as with Aurion R-Gent SE-EM. 

Aurion ECS is therefore highly recommended for single labeling experiments. It is an absolute requirement for double labeling where ECS not only reconditions specimens for the next immuno steps but also avoids the use of aldehyde fixation after the first immuno gold silver labeling sequence.

Aurion Enhancement conditioning solution
Updated: 17-04-2024
Code Pack Size Availability* Price (ex GST)
EMS25830 100ml 0 in stock (?) AU $166.00

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SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200. 

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

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Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $20
Express shipping $30

Delivery times:

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Regular shipping
Queensland   1-3 business days 
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Melbourne, Adelaide, Hobart or Canberra 

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WA, NT and non-metro areas

 4-10 business days 
Express shipping
Australia wide   1-2 business days

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Dangerous goods:

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Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping. 

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

GUARANTEE

All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods. 

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted) 

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products. 

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