NIGHTSEA Xite fluorescence flashlight system
NIGHTSEA Xite fluorescence flashlight system
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View fluorescence in the lab or the field! The Xite Fluorescence Flashlight System includes single-wavelength fluorescence excitation flashlights available with any of the five wavelengths in the NIGHTSEA line, paired with matching barrier filter glasses for maximum viewing contrast. Genotyping your transgenic organisms, screening cell cultures, inspecting parts with fluorescent penetrants, finding fluorescing critters in the field, the Xite line provides what you need.
The Xite flashlight system is replacing the Dual Fluorescent Protein (DFP) flashlight. This had been our only option for flashlights with excitation wavelengths other than Royal Blue.
The most popular DFP option was the combination of Royal Blue and Green LEDs, for excitation of GFP and red fluorescent proteins, respectively. The replacement would be the Xite-RB plus the Xite-GR. Note that the cost to purchase both of these lights is less than the price of the DFP.
Features
- Tightly focused for a high intensity illumination spot
- Two power levels, plus unique flashing mode and battery status indication
- Add-on diffuser for a broader, smoother beam
- Each light paired with matching barrier filter glasses
- Compact and lightweight
- Rechargeable high capacity lithium ion battery
Diffuser
Add-on 15° diffuser cap provides a wider, softer beam.
Available wavelengths
Wavelength sets are named and colour coded for the colour of the excitation light, not the colour of the emitted fluorescence.
Designation | Excitation | Barrier Filter Glasses |
UV - Ultraviolet | 360 - 380nm | 415nm longpass |
VI - Violet | 400 - 415nm | 450nm longpass |
RB - Royal Blue | 440 - 460nm | 500nm longpass |
RB-GO - Royal Blue, Green Only | 440 - 460nm | 500 - 560nm bandpass |
CY - Cyan | 490 - 515nm | 550nm longpass |
GR - Green | 510 - 540nm | 600nm longpass |
With the Royal Blue (RB) excitation we offer two options for filter glasses - longpass and bandpass.
Flashlight specifications
- Dimensions: 13.5cm long x 3.2cm diameter
- Weight: 255g with battery
Flashing mode for enhanced detection in ambient light
As bright as fluorescence may appear when you use an intense light source to view it in conditions of darkness, in most cases, it actually tends to be a relatively weak effect that is easily masked by other light. If you try to find fluorescence when there is moderate ambient light (room light, sunlight, etc.), any fluorescing subject will respond to the excitation, but the response may be too weak to notice easily.
But there's a trick - by making the excitation light source blink repetitively (a strobe effect), any fluorescence will blink at the same rate, while the illuminated background will not. This flickering increases the apparent contrast and thus increases detectability.
Included with each system
- Fluorescence excitation flashlight (your choice of one wavelength) with lanyard
- Matching barrier filter glasses with microfibre case for storage and cleaning
- Diffuser cap
- High capacity lithium ion battery
- Battery charger and USB charging cord
- Instruction sheet
- Padded foam shipping/carry/storage case
Store at room temperature
Note - the Xite flashlights are not suitable for underwater use