Retriever slide stainer and accessories

SKU: EMS62700-20   |   Availability ⓘ

Retriever slide stainer and accessories

SKU: EMS62700-20   |   Availability ⓘ
Part
Pack Size
P.O.R.


The unique retriever solves the problem of staining formalin fixed tissues. This is a low-cost solution to all known significant difficulties with immunohistochemistry on paraffin slices. The combination of ease of use and great consistency of findings will provide you with the highest quality immunostaining.

The Retriever is a bench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining. The model has been designed to ensure identical processing of all the samples during a processing cycle, as well as the identical processing of the samples in individual sessions. The retriever preserves processed tissues.

Now you can:

  • Run antigen unmasking in 6 various buffers at once.
  • Perform gentle antigen retrieval that does not damage the tissue morphology.
  • Get identical results every time.
  • Compare a series of slides treated in independent sessions.

Full set contains the processing unit, slide chamber (allows to fit 18 Slides) and Chamber Rack for 6 Slide Chambers (108 slides in total). 

How does the Retriever work?

Ok, basically it is a pressure cooker. However, a pressure cooker specially designed to unmask the antigen on tissue sections. The core principle is heating of the chambers with the slides at high temperature (>120?C) and high pressure. Sensors control the heating profile for the temperature and
pressure to be reached at certain pace and over certain time. We did a lot of tests to find the optimal settings.

When the required temperature is reached, it will be kept for several minutes. After that the slides will cool over 2 hours. Specially designed thermal walls of the unit control the speed of cooling of the inner chamber and slides.

Who would benefit from using our Retriever?

  • Investigative Pathology, where the high quality of staining (a picture may be published!) is required.
  • Any lab that is short on technical personnel: any student or post-doc can process slides for the staining without using much time
  • Small routine pathology labs, where a limited number of slides should be processed daily
  • Anyone who uses highly valuable samples, such as tissue arrays or unique tissue samples: Simplicity and reliability of the unit ensures the safety of your sample, and a high quality antigen unmasking

How To Use:

Step 1.

Deparaffinized sections on slides are placed into the Processing (Tissue Slide) Chambers (EMS62705-01). Retriever can accommodate simultaneously from 1 to 6 Chambers, which allows you to process a series of slides in up to 6 different antigen unmasking buffers within the same session. Fill the chambers with a buffer of your choice.

Step 2.

Place the Chambers into the Rack. Fill the Retriever with 750 ml of deionized/distilled water. Place the Rack into Retriever. Close the lid with a simple twist.

Step 3.

Push the Start button. The tissues will be processed automatically. In about two hours (depends on the load) you can open the lid and proceed with immunostaining.

Technical Specifications of Retriever:

Height 335 mm
Capacity 9 litres
Max. Instrument length 228 mm
Width 340 mm
Net Weight 4.5 kilos
Internal chamber Dimensions (d/h) 210/230 mm
Max. Load Weight 3.0kg

Other Specifications:

Fuses - Located under the control module, fuses F1 OA, 32 x 6.3mm, ceramic sand filled, Mains plug top fuse (User replaceable), F1 3A to BS1362 UK ONLY.

Rating - Models are rated continuously for intermittent use.

Body- Deep drawn aluminium. Lid - Aluminium.

Heater- Externally surface mounted mechanically fixed electric element.

Temperature Cut Out - Thermal fuse.

Pressure - Calibrated pressure release valve.

Max. Single Fault Temperature - 133.3?C

Environment Conditions - indoor use - temperature 5?C to 40?C - altitude up to 2000m - maximum relative humidity 80% for temperatures up to 31?C decreasing linearly to 50% relative humidity at 40?C. - mains supply voltage fluctuations not to exceed +10% of the nominal voltage

Input Connections - Mains inlet socket 'hot' format conforming to IEC 302.

Safety Shut Down - See "Temperature Cut Out".

Choosing A Buffer:

If you already know the buffer that can be used for Microwave treatments of sections in order to unmask you antigen of interest, the high chances are that the same buffer may be used in Retriever.

To improve the morphology of tissue of the processed sections, use one of our supplied ready-to-use buffers by choosing them according to pH.

We have commercially available the following buffers:

  • R-Buffer A pH 6.0
  • R-Buffer B pH 8.0
  • R-Buffer C pH 4.5
  • R-Buffer U pH 6.0
  • G variants of the same buffers for a more gentle processing of the tissues.

To successfully retrieve the antigen of interest on fixed sections, please remember that two factors define the choice of buffer:

  • The nature of the antigen/epitope and of the antibody used for its detection
  • The fixative used and the degree of fixation.

Correction for Fixative & Degree of fixation

The suggested protocol for processing tissues is optimized for routinely formalin-fixed and paraffin embedded material. If the tissue used for sections was insufficiently fixed, overfixed (was left in formalin for too long), or other fixative was used, the protocol may require some modifications. The easiest correction is to use EMS's own specially formulated buffers for a gentle (G) tissue processing. Use them instead of the basic buffers. For overfixed material try using U buffer or run the additional cycle in the same buffer.

Fixative Used on Tissues Buffer

Formalin, buffered R-Buffer A, B, C or U
Formalin-Zn R-Buffer A, B, C or U
Mirsky fixative Under testing
Boonfix I Under testing
Boonfix II Under testing
PLP R-buffer AG, BG, or CG

Choosing Buffer: Correction for the Nature of the Antigen and Epitope.

The choice of the buffer depends on the nature of the antigen and the location of the epitope of interest. We advise you to first run the test for the most appropriate buffer using the tissues where the expression of the antigen does occur. General guidelines for choosing the buffer:

Most of the nuclear antigens (apoptosis-related, survival-related, proliferation-related) R: Buffer A (or AG).

Cell adhesion molecules, cell membrane antigens (extracellular domain) R: Buffer A (or AG)

Cytoskeleton and cytoskeleton-associated molecules - R:Buffer A (or AG)

Intracellular domain of some adhesion molecules and surface receptors - R:Buffer B (or BG)

Intracellular domain of some adhesion molecules and surface receptors - R:Buffer C (or CG)

Most of antibodies raised against a linear peptide - R: Buffer U (or UG)

Online Operational manual

Updated: 24-04-2024
Code Part Pack Size Availability* Price (ex GST)
EMS62700-20 Slide stainer, full set 220V Each 0 in stock (?) P.O.R.
EMS62705-01 Slide chamber Pack/3 0 in stock (?) AU $382.00
EMS62705-02 Chamber rack Each 0 in stock (?) AU $241.00
EMS62705-03 Lifting device Each 0 in stock (?) AU $74.00
EMS62705-07 Green silicone sealing gasket Each 0 in stock (?) AU $198.00

REFUNDS AND RETURNS

Please ask prior to returning any goods.

Defective products should be returned to us for replacement or credit. It is best to contact support with as much detail as possible, including pictures, so we can quickly resolve the issue. ProSciTech Pty Ltd complies with the ACCC guidelines, available here: https://www.accc.gov.au/consumers/consumer-rights-guarantees/repair-replace-refund 

If you decide the item purchased does not suit your requirements, you may contact us to see if a return is possible. Some items may not be refunded, so please ask before purchase if you think a return may be necessary. The greater of $15 or 15% re-stocking charge  will be levied on materials returned due to an error on the part of the customer. We try to keep this charge minimal, however returns do have a cost to us.

SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200. 

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

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Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $20
Express shipping $30

Delivery times:

Check your email for tracking details provided at time of shipping for updates of your parcel. We cannot guarantee delivery times, as this is outside of our control. The following are the delivery estimates as given by our couriers. 

Regular shipping
Queensland   1-3 business days 
Sydney   2-5 business days 

Melbourne, Adelaide, Hobart or Canberra 

 3-10 business days 

WA, NT and non-metro areas

 4-10 business days 
Express shipping
Australia wide   1-2 business days

Urgent Orders:

Please request "Express" if you would like us to upgrade your shipment to express delivery. Providing your order fits in a satchel and/or is under 1kg in weight, we will ship your order either DHL, TNT or Express Post. Larger or heavy orders need a quotation for additional freight costs.

Dangerous goods:

See the table on the page for DG shipment costs by road. Please specify when ordering if you require air service (this can be prohibitively expensive). Please request a quotation. We will charge at cost.
 

Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping. 

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

GUARANTEE

All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods. 

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted) 

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products. 

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