Fluorescent Particles

Fluorescent particles with single or multiple fluorophores are available in various sizes, emission spectra and combinations. Many are suitable for uses in flow cytometry, fluorescence microscopy, phagocytosis studies and cell labelling. Please contact us for fluorescent particle recommendations.

Fluorescent polystyrene particles typically have a high fluorescence intensity, which means that they emit a bright signal when excited by light of a specific wavelength. These particles are often used in applications where strong fluorescent signals are required, such as in fluorescence microscopy, flow cytometry, and in vitro diagnostic assays.

Low-intensity fluorescent polystyrene particles, on the other hand, have a relatively weaker fluorescence signal compared to their high-intensity counterparts. These particles are often used in applications where a low fluorescent signal is desired, such as in calibration standards, quality control, and instrument calibration. Low-intensity fluorescent particles are also useful in reducing background fluorescence and minimizing signal interference.

The SPHERO™ Fluorescent Microparticles are prepared by either staining the polystyrene core of the particles with a solution of appropriate fluorophore or by polymerizing a fluorophore in styrene in the presence of polystyrene core particles. As a result, a wide variety of fluorescent particles can be prepared ranging in size, type of fluorophore, fluorescence intensity and surface functional groups. Most of the fluorophores chosen for use in the preparation of SPHERO™ Fluorescent Particles are water insoluble and therefore are very stable. These fluorophores, once incorporated into the particles, do not leach and their colour and fluorescence remains stable for long periods of time under proper storage conditions.