Citifluor CFPVOH

SKU: EMSCFP-25   |   Availability ⓘ

Citifluor CFPVOH

SKU: EMSCFP-25   |   Availability ⓘ
Pack Size
AU $88.00 Regular price

Taxes and shipping applied at checkout.

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Designed to reduce the fading of the fluorescence of dyes used for labelling biological specimens.
In fluorescence microscopy, the fluorescence is stimulated by high intensity UV and/or visible light. Absorption of the light creates an excited state of the dye (usually a singlet state) and this leads to fluorescence. However, the excited state of the dye may undergo chemical reactions which lead to its destruction as evidenced by the fading or bleaching of the fluorescence and consequent loss of the image, or in the case of assays, a change in signal intensity during measurement.

There is no universal anti-fadent; due to the varied nature of biological material the fluorescence fading of fluorochromic conjugates is a complex process.
The general anti-fadents are AF1, AF2 and AF3. AF87 is a specialist immersion oil-based anti-fadent.

Guide to Non-Hardening Mountants

Glycerol Based
  • AF1 A glycerol-PBS solution contains an amine antifadent.
  • AF1 plus DAPI contains both an amine antifadent and the DNA stain, DAPI.
  • AF2 A glycerol solution contains an amine antifadent that allows you to choose your own buffer solution.
  • AF4 A glycerol solution of n-propyl gallate.
Glycerol Free
  • AF3 is a PBS solution containing an amine-based antifadent.
  • CFPVOH is an aqueous solution of poly (vinyl alcohol). Provided care is taken not to allow the water to evaporate, it will remain fluid. Retardation of photobleaching is effected by the addition of the aqueous antifadent solution AF100.
  • AF100 Is a PBS solution of an amine-based antifadent that if used as an additive to CFPVOH (1 part AF100 to 9 parts CFPVOH). Provided care is taken not to allow the water to evaporate, it will remain fluid.
  • AFR3 is a PBS solution containing a NEW non-amine, non phenolic antifadent.


Guide to Hardening Mountants

Hardening mountants are aqueous solutions containing a polymer such a poly (vinyl alcohol) (PVOH). When a few microlitres of these solutions are pipetted onto a microscope slide and a coverslip applied, evaporation of water slowly takes place and the formation of a stable film results which immobilises the coverslip.

A variety of hardening mountants is available which provide films possessing a range of hardness. They are based on water-soluble polymers such as poly(vinyl alcohol(PVOH) and poly (vinyl pyrrolidone)(PVP).

Why are aqueous glycerol solutions of PVOH containing antifadents inherently unstable?

PVOH is produced by hydrolysis of poly(vinyl acetate) and most commercial samples of PVOH contain residual (unhydrolysed) acetate groups. These groups undergo hydrolysis, often accelerated by the added antifadents, during storage and this can cause a change in pH and more usually gellation.

This latter process leads to an unpredictable shelf-life. By making up small volumes of PVOH solution containing the required amount of antifadent solution, you have materials of consistent composition and performance as well as making better use of your purchased materials.

Glycerol Based
  • AF200 is a glycerol solution containing an amine-based antifadent.
  • AF300 is a glycerol solution containing a phenolic type of antifadent.
Film Forming Polymer Solutions
  • Tris-MWL 4-88 is a classical, popular mountant solution based on Mowiol 4-88, glycerol, water and tris-amine buffer. Following evaporation of the water a film of weak to medium strength is formed. To have effective reduction in photobleaching, it should be used with either AF100, AF200 or AF300.
  • CFPVOH is an aqueous solution of poly (vinyl alcohol) and is designed to be used with the glycerol-based antifadent solutions AF200 or AF300. Following evaporation of the water films of medium hardness are produced.
Glycerol Free
  • PVP plus antifadent is an aqueous solution of poly (vinyl pyrrolidone) containing an amine-based antifadent. The solutions are stable over long periods e.g. in excess of five years.
  • CFPVOH plus antifadent is an aqueous solution of a carefully selected PVOH containing an amine-based antifadent and has a shelf life 6 months.
Film Forming Polymer Solutions
  • CFPVOH is an aqueous solution of poly (vinyl alcohol). Retardation of photobleaching is effected by the addition of the aqueous antifadent solution AF100. (1 part AF100 to 9 parts CFPVOH)

SDS - EMSCFP-25

Updated: 29-03-2024
Code Pack Size Availability* Price (ex GST)
EMSCFP-25 25ml 0 in stock (?) AU $88.00

REFUNDS AND RETURNS

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Defective products should be returned to us for replacement or credit. It is best to contact support with as much detail as possible, including pictures, so we can quickly resolve the issue. ProSciTech Pty Ltd complies with the ACCC guidelines, available here: https://www.accc.gov.au/consumers/consumer-rights-guarantees/repair-replace-refund 

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SHIPPING INFORMATION

Shipping and Handling charges are $20 for Australian orders; free for orders over $200. 

In-stock items are generally sent same day. Orders are shipped shipped from Townsville, Queensland. Our couriers pick up from 2:30pm, so please place your order in plenty of time for processing and packing if you would like it sent same day. When items are not in stock, your order may be part-shipped. Items on back-order will be sent as soon as they become available.

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Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $20
Express shipping $30

Delivery times:

Check your email for tracking details provided at time of shipping for updates of your parcel. We cannot guarantee delivery times, as this is outside of our control. The following are the delivery estimates as given by our couriers. 

Regular shipping
Queensland   1-3 business days 
Sydney   2-5 business days 

Melbourne, Adelaide, Hobart or Canberra 

 3-10 business days 

WA, NT and non-metro areas

 4-10 business days 
Express shipping
Australia wide   1-2 business days

Urgent Orders:

Please request "Express" if you would like us to upgrade your shipment to express delivery. Providing your order fits in a satchel and/or is under 1kg in weight, we will ship your order either DHL, TNT or Express Post. Larger or heavy orders need a quotation for additional freight costs.

Dangerous goods:

See the table on the page for DG shipment costs by road. Please specify when ordering if you require air service (this can be prohibitively expensive). Please request a quotation. We will charge at cost.
 

Shipping to New Zealand Destinations

Orders are now shipped 'receipted delivery'.

Charges:

Order Value Shipping and Handling charges
Greater than $200 - (exceptionally heavy or Dangerous Goods excluded). Free
Less than $200 $26 Minimum Charge

Heavy items (e.g. benchroll or large quantities of microscope slides) may require an additional freight charge - please ask for a quote.

DANGEROUS GOODS shipped to New Zealand: Dangerous goods will be air-freighted and the freight charge billed at cost. Typically, this will be about AUD $600AUD to New Zealand (Up to 2kg).

Delivery times: by air with signature on delivery, depending on courier, delivery can take 3-14 working days. Customs delays are unpredictable and can extend the delivery. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

Shipping to Rest of World Destinations

Contact us for a quote for shipping. 

Delivery times: by air with signature on delivery, depending on courier, delivery can be 7-21 working days. Customs delays are unpredictable and can extend the delivery time. Due to COVID19 delivery times have been extended through the couriers and are out of our control.

DANGEROUS GOODS shipped outside Australia: Not available

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All products are guaranteed as advertised. Defective products should be returned to us for replacement or credit. Please contact us prior to returning goods. 

We endeavour to provide accurate descriptions, illustrations, prices and stock availability, but errors and omissions are possible. All quotations are E&OE (Errors and Omissions Excepted) 

LIABILITY

All products are intended for laboratory applications only and the user assumes all responsibility involved in use and handling. We accept no liability for loss or damage resulting from the use or abuse of our products. 

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Trouble Shooting

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