The Cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.
Cryo 35° and Cryo 45°
The 45° knife is well suited for routine cryo sectioning.
The 35° knife allows sectioning with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).
The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.
The seal is made of a cold-resistant epoxy resin.
** Resharpened DiATOME knives undergo the same stringent optical checking and sectioning tests as new knives. If you are wanting a re-sharpened service, you will post your old knife to us and we will send away to our supplier. Lead time ~ 6-10 weeks from receipt of your knife.