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Forms a thin, conductive, moisture barrier between specimens and a vacuum environment
Taking electron microscope images of biological objects in their natural state
What is the NanoSuit?
A novel technology which enables us to observe cells, microorganisms, etc. in a living state using scanning electron microscopy (SEM).
NanoSuit forms a very thin barrier layer on the surface of an object and the barrier layer holds moisture in the object under vacuum condition in electron microscopy. Also, the barrier layer is electrically conductive.
Therefore, NanoSuit makes it possible to observe biological objects with their natural image texture.
NanoSuit is very easy to use. Only dropping the NanoSuit solution onto the objects, then you can observe the objects using SEM. (You don't need any other fixation procedures.)
Inventor Professor Takahiko Hariyama tried to observe various microorganisms using SEM, but almost all organisms died due to losing moisture under the vacuum condition in the electron microscopy.
However, he discovered a kind of fly larva was able to keep alive under the vacuum condition.
Prof. Hariyama investigated that phenomenon and revealed that a very thin barrier layer was formed on the surface of the larva by electron beam irradiation. The barrier layer held moisture and kept the larva alive.
Hariyama and his colleagues had tried to mimic this phenomenon artificially and invented "NanoSuit" technology.
Aqueous solution of the biocompatible polymer. (biologically safe material)
Just drop onto the objects.
Polymerize by electron beam or plasma irradiation, then a thin barrier layer is formed on the surface of the objects.
The barrier layer holds moisture contained in the objects and its conductivity provides clear SEM images.
Example (flower petal)
Under the vacuum condition, the petals are dried and do not retain the original shape.
NanoSuit keeps the petal moist under the vacuum condition and provides natural shape images of the fine structure of the petal surface.
With NanoSuit, you can observe the minute structure of the insect's body surface with a simple operation.
Example (living tissues)
Nanosuit keeps the texture of living cells and enables to distinguish between cancer tissue and normal tissue.
Example (chicken drumsticks)
The surface of the chicken drumsticks (Fresh) is protected by NanoSuit solution and observed by FE-SEM with high resolution shows fibrous microstructures of uniform size.
On the other hand, in (Freeze-thaw) examined by the same method, it was observed that the fine structure of the surface was broken and liquid substances were seeping out to the surface.
These images suggest that such a difference in surface condition may be the cause of the dry feeling of the thawed food.